Cite this paper:
HU Shunxin, WANG You, WANG Ying, ZHAO Yan, ZHANG Xinxin, ZHANG Yongsheng, JIANG Ming, TANG Xuexi. Effects of elevated pCO2 on physiological performance of marine microalgae Dunaliella salina (Chlorophyta, Chlorophyceae)[J]. HaiyangYuHuZhao, 2018, 36(2): 317-328

Effects of elevated pCO2 on physiological performance of marine microalgae Dunaliella salina (Chlorophyta, Chlorophyceae)

HU Shunxin1, WANG You1, WANG Ying1, ZHAO Yan1, ZHANG Xinxin1, ZHANG Yongsheng1,2, JIANG Ming1, TANG Xuexi1
1 College of Marine Life Science, Ocean University of China, Qingdao 266003, China;
2 Rongcheng Ocean and Fisheries Bureau, Weihai 264300, China
Abstract:
The present study was conducted to determine the effects of elevated pCO2 on growth, photosynthesis, dark respiration and inorganic carbon acquisition in the marine microalga Dunaliella salina. To accomplish this, D. salina was incubated in semi-continuous cultures under present-day CO2 levels (390 μatm, pHNBS:8.10), predicted year 2100 CO2 levels (1 000 μatm, pHNBS:7.78) and predicted year 2300 CO2 levels (2 000 μatm, pHNBS:7.49). Elevated pCO2 significantly enhanced photosynthesis (in terms of gross photosynthetic O2 evolution, effective quantum yield (△F/F'm), photosynthetic efficiency (α), maximum relative electron transport rate (rETRmax) and ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) activity) and dark respiration of D. salina, but had insignificant effects on growth. The photosynthetic O2 evolution of D. salina was significantly inhibited by the inhibitors acetazolamide (AZ), ethoxyzolamide (EZ) and 4,4'-diisothiocyanostilbene-2,2'-disulfonate (DIDS), indicating that D. salina is capable of acquiring HCO3- via extracellular carbonic anhydrase and anion-exchange pr 3 oteins. Furthermore, the lower inhibition of the photosynthetic O2 evolution at high pCO2 levels by AZ, EZ and DIDS and the decreased carbonic anhydrase showed that carbon concentrating mechanisms were down-regulated at high pCO2. In conclusion, our results show that photosynthesis, dark respiration and CCMs will be affected by the increased pCO2/low pH conditions predicted for the future, but that the responses of D. salina to high pCO2/low pH might be modulated by other environmental factors such as light, nutrients and temperature. Therefore, further studies are needed to determine the interactive effects of pCO2, temperature, light and nutrients on marine microalgae.
Key words:    ocean acidification|growth|photosynthesis|CO2|CCMs|Dunaliella salina   
Received: 2016-10-08   Revised:
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Articles by HU Shunxin
Articles by WANG You
Articles by WANG Ying
Articles by ZHAO Yan
Articles by ZHANG Xinxin
Articles by ZHANG Yongsheng
Articles by JIANG Ming
Articles by TANG Xuexi
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