Cite this paper:
HE Jiaying, WANG Kai, XIONG Jinbo, GUO Annan, ZHANG Demin, FEI Yuejun, YE Xiansen. Drivers of coastal bacterioplankton community diversity and structure along a nutrient gradient in the East China Sea[J]. HaiyangYuHuZhao, 2018, 36(2): 329-340

Drivers of coastal bacterioplankton community diversity and structure along a nutrient gradient in the East China Sea

HE Jiaying1,2, WANG Kai1,2, XIONG Jinbo1,2, GUO Annan1, ZHANG Demin1,2, FEI Yuejun3, YE Xiansen3
1 School of Marine Sciences, Ningbo University, Ningbo 315211, China;
2 Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, Ningbo 315200, China;
3 Marine Environmental Monitoring Center of Ningbo, State Oceanic Administration(SOA), Ningbo 315211, China
Anthropogenic nutrient discharge poses widespread threats to coastal ecosystems and has increased environmental gradients from coast to sea. Bacterioplankton play crucial roles in coastal biogeochemical cycling, and a variety of factors affect bacterial community diversity and structure. We used 16S rRNA gene pyrosequencing to investigate the spatial variation in bacterial community composition (BCC) across five sites on a coast-offshore gradient in the East China Sea. Overall, bacterial alpha-diversity did not differ across sites, except that richness and phylogenetic diversity were lower in the offshore sites, and the highest alpha-diversity was found in the most landward site, with Chl-a being the main factor. BCCs generally clustered into coastal and offshore groups. Chl-a explained 12.3% of the variation in BCCs, more than that explained by either the physicochemical (5.7%) or spatial (8.5%) variables. Nutrients (particularly nitrate and phosphate), along with phytoplankton abundance, were more important than other physicochemical factors, co-explaining 20.0% of the variation in BCCs. Additionally, a series of discriminant families (primarily affiliated with Gammaproteobacteria and Alphaproteobacteria), whose relative abundances correlated with Chl-a, DIN, and phosphate concentrations, were identified, implying their potential to indicate phytoplankton blooms and nutrient enrichment in this marine ecosystem. This study provides insight into bacterioplankton response patterns along a coast-offshore gradient, with phytoplankton abundance increasing in the offshore sites. Time-series sampling across multiple transects should be performed to determine the seasonal and spatial patterns in bacterial diversity and community structure along this gradient.
Key words:    bacterioplankton|pyrosequencing|nutrient gradient|phytoplankton abundance|dominant factor|bioindicator   
Received: 2016-04-17   Revised:
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