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MA Yuexin, TAO Wei, LIU Jiao, LIU Changfa, LI Jin, LIU Jichen. Response of ammonia-oxidizing betaproteobacteria to shortterm fertilization in a salt marsh in China[J]. HaiyangYuHuZhao, 2018, 36(2): 351-361

Response of ammonia-oxidizing betaproteobacteria to shortterm fertilization in a salt marsh in China

MA Yuexin1, TAO Wei2, LIU Jiao1, LIU Changfa2, LI Jin3, LIU Jichen1
1 Key Laboratory of Mariculture and Stock Enhancement in North China's Sea of Ministry of Agriculture, Dalian Ocean University, Dalian 116023, China;
2 Key Laboratory of Marine Environmental Research of Liaoning Higher Education, Dalian 116023, China;
3 Institute of Ocean and Fisheries of Panjin, Panjin 124001, China
This study examines the impacts of short-term (6 months) fertilization on the community structure and abundance of ammonia-oxidizing betaproteobacteria (β-AOB) and the potential nitrification rate in sediment colonized by Suaeda heteroptera in a saltmarsh located in Shuangtai estuary, China. The sediment samples were collected from plots treated with different amounts of an N fertilizer (urea supplied at 0.1, 0.2, 0.4, and 0.8 g/kg (nitrogen content in dry sediment)), and with different forms of N fertilizers (urea, (NH4)2SO4, and NH4NO3, each supplied at 0.2 g/kg). The fertilizers were applied 1-4 times during the plant-growing season in May, July, August and September of 2013. Untreated plots were included as a control. As revealed in denaturing gradient gel electrophoresis of the 16S rRNA gene, the β-AOB community responded to both the amount and form of N. Real-time quantitative PCR indicated that both abundance and potential nitrification rate of β-AOB increased after N addition, regardless of concentration and form (except NH4NO3). These results provide evidence that short-term N application influences the sediment β-AOB community, β-AOB abundance and potential nitrification rate in a saltmarsh ecosystem.
Key words:    ammonia-oxidizing betaproteobacteria (β-AOB)|denaturing gradient gel electrophoresis (DGGE)|nitrification|fertilization|saltmarsh   
Received: 2016-05-26   Revised:
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