Transcriptome profiling analysis of <i>Mactra veneriformis</i> by deep sequencing after exposure to 2,2',4,4'-tetrabromodiphenyl ether -- Journal of Oceanology and Limnology,2018,36(2):490-507

	Cite this paper:
	SHI Pengju, DONG Shihang, ZHANG Huanjun, WANG Peiliang, NIU Zhuang, FANG Yan. Transcriptome profiling analysis of Mactra veneriformis by deep sequencing after exposure to 2,2',4,4'-tetrabromodiphenyl ether[J]. Journal of Oceanology and Limnology, 2018, 36(2): 490-507

Transcriptome profiling analysis of Mactra veneriformis by deep sequencing after exposure to 2,2',4,4'-tetrabromodiphenyl ether

SHI Pengju¹, DONG Shihang¹, ZHANG Huanjun², WANG Peiliang³, NIU Zhuang¹, FANG Yan¹

1 School of Agriculture, Ludong University, Yantai 264025, China;
2 Shandong Marine Resource and Environment Research Institute, Shandong Provincial Key Laboratory of Restoration for Marine Ecology, Yantai 264006, China;
3 Weihai Hongrun Marine Technology Co. Ltd., Weihai 264200, China

Abstract:

Polybrominated diphenyl ethers (PBDEs) are ubiquitous global pollutants, which are known to have immune, development, reproduction, and endocrine toxicity in aquatic organisms, including bivalves. 2,2',4,4'-Tetrabromodiphenyl ether (BDE-47) is the predominant PBDE congener detected in environmental samples and the tissues of organisms. However, the mechanism of its toxicity remains unclear. In this study, high-throughput sequencing was performed using the clam Mactra veneriformis, a good model for toxicological research, to clarify the transcriptomic response to BDE-47 and the mechanism responsible for the toxicity of BDE-47. The clams were exposed to 5 μg/L BDE-47 for 3 days and the digestive glands were sampled for high-throughput sequencing analysis. We obtained 127 648, 154 225, and 124 985 unigenes by de novo assembly of the control group reads (CG), BDE-47 group reads (BDEG), and control and BDE-47 reads (CG & BDEG), respectively. We annotated 32 176 unigenes from the CG & BDEG reads using the NR database. We categorized 24 401 unigenes into 25 functional COG clusters and 21 749 unigenes were assigned to 259 KEGG pathways. Moreover, 17 625 differentially expressed genes (DEGs) were detected, with 10 028 upregulated DEGs and 7 597 downregulated DEGs. Functional enrichment analysis showed that the DEGs were involved with detoxification, antioxidant defense, immune response, apoptosis, and other functions. The mRNA expression levels of 26 DEGs were verified by quantitative real-time PCR, which demonstrated the high agreement between the two methods. These results provide a good basis for future research using the M. veneriformis model into the mechanism of PBDEs toxicity and molecular biomarkers for BDE-47 pollution. The regulation and interaction of the DEGs would be studied in the future for clarifying the mechanism of PBDEs toxicity.

Key words: transcriptomic response|Mactra veneriformis|2,2',4,4'-tetrabromodiphenyl ether

Received: 2016-12-27 Revised:

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