Assessment of negative phototaxis in long-term fasted <i>Glyptocidaris crenularis</i>: a new insight into measuring stress responses of sea urchins in aquaculture -- Journal of Oceanology and Limnology,2015,33(1):37-44

	Cite this paper:
	TIAN Xiaofei, WEI Jing, ZHAO Chong, FENG Wenping, SUN Ping, CHANG Yaqing. Assessment of negative phototaxis in long-term fasted Glyptocidaris crenularis: a new insight into measuring stress responses of sea urchins in aquaculture[J]. Journal of Oceanology and Limnology, 2015, 33(1): 37-44

Assessment of negative phototaxis in long-term fasted Glyptocidaris crenularis: a new insight into measuring stress responses of sea urchins in aquaculture

TIAN Xiaofei, WEI Jing, ZHAO Chong, FENG Wenping, SUN Ping, CHANG Yaqing

Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture, Dalian Ocean University, Dalian 116023, China

Abstract:

A cost-effective method was designed to measure the behavioral response of negative phototaxis to high-intensity illumination in the sea urchin Glyptocidaris crenularis. Ninety sea urchins were randomly and equally divided into two aquaculture environment groups: a fasted group, which was starved during the experiment, and a fed group. After 10 months, the total mortality of each group was recorded. Then, 15 sea urchins were randomly selected from each group and behavioral responses to high-intensity illumination were investigated for each sea urchin. After the behavioral experiment, body measurements of the trial sea urchins were taken. The results reveal that food deprivation significantly affected test diameter (P <0.01), body weight (P <0.01), gonad weight (P <0.01), and gut weight (P <0.01). Furthermore, food deprivation also affected negative phototaxis behaviors of time to rapid spine movement (P <0.01), time to the 1 cm position (P <0.05), and walking distance in 300 s (P <0.01), but not time to body reaction (P >0.05). The mortality rates of fasted and fed urchins were 6.7% and 0%, respectively. The present study provides evidence that food deprivation has a significant effect on phenotypic traits and behavioral responses to high-intensity illumination in the sea urchin G. crenularis. With this method, environmental stressors can be easily detected by measuring proper optional indicators. This study provides a new insight into measuring stress responses of sea urchins in aquaculture. However, further studies should be carried out to understand more environmental factors and to compare this potential behavioral method with immune, physiological, and epidemiological approaches.

Key words: sea urchin|negative phototaxis|aquaculture|food deprivation|stress

Received: 2013-12-10 Revised: 2014-04-23

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Articles by TIAN Xiaofei

Articles by WEI Jing

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Articles by SUN Ping

Articles by CHANG Yaqing

References:
Bayne B L. 1985. Responses to environmental stress: tolerance, resistance and adaptation. In: Bayne B L ed. Marine Biology of Polar Regions and Effects of Stress on Marine Organisms. Wiley, London.
Bayne C J, Gerwick L. 2001. The acute phase response and innate immunity of fish. Dev. Comp. Immuno., 25 (8): 725-743.
Boyd C E, Schmittou H R. 1999. Achievement of sustainable aquaculture through environmental management. Aquac. Econ. Manag., 3 (1): 59-69.
Branco P C, Borges J C S, Santos M F et al. 2013. The impact of rising sea temperature on innate immune parameters in the tropical subtidal sea urchin Lytechinus variegatus and the intertidal sea urchin Echinometra lucunter. Mar. E nviron. R es., 92: 95-101.
Branco P C, Pressinotti L N, Borges J C S et al. 2012. Cellular biomarkers to elucidate global warming effects on Antarctic sea urchin Sterechinus neumayeri. Polar B iol., 35 (2): 221-229.
Cenni F, Parisi G, Scapini F, Gherardi F. 2010. Sheltering behavior of the abalone, Haliotis tuberculata L. in artificial and natural seawater: the role of calcium. Aquaculture, 299: 67-72.
Chang Y, Ding J, Song J, Yang W. 2004. Biology and Aquaculture of Sea Cucumbers and Sea Urchins. Ocean Press, Beijing, China. p.27-217. (in Chinese)
Constable A J. 1993. The role of sutures in shrinking of the test in Heliocidaris erythrogramma (Echinoidea: Echinometridae). Mar. Biol., 117: 423-430.
Downs C A, Fauth J E, Woodley C M. 2001. Assessing the health of grass shrimp (Palaeomonetes pugio) exposed to natural and anthropogenic stressors: a molecular biomarker system. Mar. Biotechnol., 3 (4): 380-397.
Dubois P, Chen C P. 1989. Calcification in echinoderms. Echinoderm. Stud., 3: 109-178.
Ebert T A. 1967. Negative growth and longevity in the purple sea urchin Stongylocentrotus purpuratus (Stimpson). Science, 157: 557-558.
Ebert T A. 1968. Growth rates of the sea urchin Strongylocentrotus purpuratus related to food availability and spine abrasion. Ecology, 49: 1 075-1 091.
Filosto S, Roccheri M C, Bonaventura R et al. 2008. Environmentally relevant cadmium concentrations affect development and induce apoptosis of Paracentrotus lividus larvae cultured in vitro. Cell B iol. T oxicol., 24 (6): 603-610.
Hoegh-Guldberg O, Bruno J F. 2010. The impact of climate change on the world's marine ecosystems. Science, 328 (5985): 1 523-1528.
Kienle C, Köhler H R, Filser J, Gerhardt A. 2008. Effects of nickel chloride and oxygen depletion on behaviour and vitality of zebrafish (Danio rerio, Hamilton, 1822) (Pisces, Cypriniformes) embryos and larvae. Environ. Pollut., 152: 612-620.
Lares M T, Pomory C M. 1998. Use of body components during starvation in Lytechinus variegatus (Lamarck) (Echinodermata: Echinoidea). J. Exp. Marine Biol. Ecol., 225 (1): 99-106.
Lehtonen H. 1996. Potential effects of global warming on northern European freshwater fish and fisheries. Fish. Manag. Ecol., 3 (1): 59-71.
Lesser M P, Carleton K L, Böttger S A, Barry T M, Walker C W. 2011. Sea urchin tube feet are photosensory organs that express a rhabdomeric-like opsin and PAX6. Proc. R. Soc. B., 278: 3 371-3 379.
Levitan D R. 1988. Density-dependent size regulation and negative growth in the sea urchin Diadema antillarum Philippi. Oecologia, 76: 627-629.
Levitan D R. 1991. Skeletal changes in the test and jaws of the sea urchin Diadema antillarum in response to food limitation. Mar. Biol., 111: 431-435.
Luo S, Tian X, Zhao C, Zhou H, Zhang W, Feng W, Chang Y. 2014. Phenotypic correlations of somatic and gonad traits of sea urchins Glyptocidaris crenularis in two sampled periods: first insight into its breeding and aquaculture. C hin. J. O ceanol. L imn., 32 (2): 1-5.
McCarron E, Burnell G, Mouzakitis G. 2009. Growth assessment on three size classes of the purple sea urchin Paracentrotus lividus using continuous and intermittent feeding regimes. Aquaculture, 288: 83-91.
Noga E J, Ullal A J, Corrales J, Femandes J M O. 2011. Application of antimicrobial polypeptide host defenses to aquaculture: exploitation of downregulation and upregulation responses. C omp. B iochem. P hys. D., 6 (1): 44-54.
Pearse J S. 2006. Ecological role of purple sea urchins. Science, 314: 940-941.
Pinsino A, Della Torre C, Sammarini V et al. 2008. Sea urchin coelomocytes as a novel cellular biosensor of environmental stress: a field study in the Tremiti Island Marine Protected Area, Southern Adriatic Sea, Italy. Cell B iol. T oxicol., 24 (6): 541-552.
Robinson N, Smith B, Cooke I, Strugnell J. 2013. A snail's pace: a preliminary analysis of the effects of stress and genetics on movement of Haliotis. Aquaculture, 25 (35): 376-379.
Robohm R A, Draxler A F J, Wieczorek D, Kapareiko D, Pitchford S. 2005. Effects of environmental stressors on disease susceptibility in American lobsters: a controlled laboratory study. J. Shellfish. Res., 24 (3): 773-779.
Ryan R M, Frederick C. 1997. On energy, personality, and health: subjective vitality as a dynamic reflection of wellbeing. J. Per. Soci. Psycho., 530-561.
Schreck C B, Lorz H W. 1978. Stress response of coho salmon (Oncorhynchus kisutch) elicited by cadmium and copper and potential use of cortisol as an indicator of stress. J. Fish. Board. Can., 35 (8): 1 124-1 129.
Tian D, Li N, Huang H, Fu Z, Zhang X. 2009. A decision support system for evaluating quality safety risk contaminated by water pollution in aquaculture pond. Computer and Computing Technologies in Aquaculture II, 293: 643-652.
Tseng C K, Wu C Y, Ren K Z. 1962. The influence of temperature on the growth and development of Haidai (Laminaria japonica) gametophytes. Oceanol. et Limnol. Sinica., 4 (1-2): 22-28.
Viarengo A, Canesi L. 1991. Mussels as biological indicators of pollution. Aquaculture, 94 (2): 225-243.
Yoshida M. 1957. Positive phototaxis in Psammechinus microtuberculatus (Blainville). Publ. Staz. Zool. Napoli., 30: 260-262.
Yoshida M. 1962. The effect of light on the shadow reaction of the sea urchin, Diadema setosum (Leske). J. Exp. Biol., 39 (4): 589-602.
Yoshimura K, Motokawa T. 2010. Bilaterality in the regular sea urchin Anthocidaris crassispina is related to efficient defense not to efficient locomotion. Mar. Biol. Res., 11 (157): 2 475-2 488.
Zhang W, Zhao C, Luo S, Chang Y, Song J. 2011. Design of a cost-effective facility for family breeding programs of marine organisms. Aquacult. Engine., 45: 146-147.
Zhao C, Li X, Luo S, Chang Y. 2011. Assessments of lysosomal membrane responses to stresses with neutral red retention assay and its potential application in the improvement of bivalve aquaculture. Afr. J. Biotechnol., 10 (64): 13 968- 13 973.
Zhao C, Zhang W, Chang Y, Zhou H, Song J, Luo S. 2013. Effects of continuous and diel intermittent feeding regimes on food consumption, growth and gonad production of the sea urchin Strongylocentrotus intermedius of different size classes. Aquacult. Int., 21: 699-708.