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SUN Yuhao, CHEN Xiaolin, LIU Song, YU Huahua, LI Rongfeng, WANG Xueqin, QIN Yukun, LI Pengcheng. Preparation of low molecular weight Sargassum fusiforme polysaccharide and its anticoagulant activity[J]. Journal of Oceanology and Limnology, 2018, 36(3): 882-891 |
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Preparation of low molecular weight Sargassum fusiforme polysaccharide and its anticoagulant activity |
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| SUN Yuhao1,2, CHEN Xiaolin1, LIU Song1, YU Huahua1, LI Rongfeng1, WANG Xueqin1, QIN Yukun1, LI Pengcheng1 |
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1 Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;
2 University of Chinese Academy of Sciences, Beijing 100049, China |
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| Abstract: |
| Heparin has been used as an anticoagulant drug for many years, but it has significant side effects. In the search for good substitutes, low molecular weight (MW) polysaccharides from Sargassum fusiforme have been examined and confirmed to possess biological activities. Here, S. fusiforme polysaccharides (SFP) were extracted and subjected to a hydrogen peroxide (H2O2) oxidation method for the preparation of low-MW SFP (LSFP). The effects of temperature, pH, and H2O2 concentration on the degradation process were also examined. Several LSFP of 36, 9, 5.7, and 2.7 kDa were obtained under different conditions, and their anticoagulant activities studied in vitro. The results showed that SFP and LSFP prolonged activated partial thromboplastin (APTT), prothrombin (PT) and thrombin times (TT) significantly, indicating that these low MW polysaccharides possessed anticoagulant activity in the intrinsic, extrinsic, and common coagulation pathways. As these effects were related to the MW of the polysaccharides in APTT and TT but not in PT, the contents of the monosaccharide fucose and sulfate and the polysaccharide MW could have exerted combined effects. The details of this mechanism require further verification. |
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| Key words:
anticoagulant activity|Sargassum fusiforme|low molecular weight|degradation
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| Received: 2017-03-20 Revised: |
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