Molecular cloning and transcriptional analysis of a NPY receptor-like in common Chinese cuttlefish <i>Sepiella japonica</i> -- Journal of Oceanology and Limnology,2018,36(3):892-904

	Cite this paper:
	YANG Jingwen, XU Yuchao, XU Ke, PING Hongling, SHI Huilai, LÜ Zhenming, WU Changwen, WANG Tianming. Molecular cloning and transcriptional analysis of a NPY receptor-like in common Chinese cuttlefish Sepiella japonica[J]. Journal of Oceanology and Limnology, 2018, 36(3): 892-904

Molecular cloning and transcriptional analysis of a NPY receptor-like in common Chinese cuttlefish Sepiella japonica

YANG Jingwen¹, XU Yuchao¹, XU Ke¹, PING Hongling², SHI Huilai², LÜ Zhenming¹, WU Changwen¹, WANG Tianming¹

1 National Engineering Research Center for Facilitated Marine Aquaculture, Marine Science College, Zhejiang Ocean University, Zhoushan 316022, China;
2 Marine Fisheries Research Institute of Zhejiang Province, Zhoushan 316022, China

Abstract:

Neuropeptide Y (NPY) has a pivotal role in the regulation of many physiological processes. In this study, the gene encoding a NPY receptor-like from the common Chinese cuttlefish Sepiella japonica (SjNPYR-like) was identified and characterized. The full-length SjNPYR-like cDNA was cloned containing a 492-bp of 5' untranslated region (UTR), 1 182 bp open reading frame (ORF) encoding a protein of 393 amino acid residues, and 228 bp of 3' UTR. The putative protein was predicted to have a molecular weight of 45.54 kDa and an isoelectric point (pI) of 8.13. By informatic analyses, SjNPYR-like was identified as belonging to the class A G protein coupled receptor (GPCR) family (the rhodopsin-type). The amino acid sequence contained 12 potential phosphorylation sites and five predicted N-linked glycosylation sites. Multiple sequence alignment and 3D structure modeling were conducted to clarify SjNPYR bioinformatics characteristics. Phylogenetic analysis identifies it as an NPYR with identity of 33% to Lymnaea stagnalis NPFR. Transmembrane properties of SjNPYR-like were demonstrated in vitro using HEK293 cells and the pEGFP-N1 plasmid. Relative quantification of SjNPYR-like mRNA level confirmed a high level expression and broad distribution of SjNPYR-like in various tissues of female S. japonica. In addition, the transcriptional profile of SjNPYR-like in the brain, liver, and ovary during gonadal development was analyzed. The results provide basic understanding on the molecular characteristics of SjNPYR-like and its potentially physical functions.

Key words: Sepiella japonica|NPY receptor-like|growth|reproduction|gene expression

Received: 2016-10-14 Revised:

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Articles by YANG Jingwen

Articles by XU Yuchao

Articles by XU Ke

Articles by PING Hongling

Articles by SHI Huilai

Articles by LÜ Zhenming

Articles by WU Changwen

Articles by WANG Tianming

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