Denitrification potential evaluation of a newly indigenous aerobic denitrifier isolated from largemouth bass <i>Micropterus salmoides</i> culture pond -- Journal of Oceanology and Limnology,2018,36(3):913-925

	Cite this paper:
	WANG Cuicui, ZHANG Kai, XIE Jun, LIU Qigen, YU Deguang, WANG Guangjun, YU Ermeng, GONG Wangbao, LI Zhifei. Denitrification potential evaluation of a newly indigenous aerobic denitrifier isolated from largemouth bass Micropterus salmoides culture pond[J]. Journal of Oceanology and Limnology, 2018, 36(3): 913-925

Denitrification potential evaluation of a newly indigenous aerobic denitrifier isolated from largemouth bass Micropterus salmoides culture pond

WANG Cuicui^1,2, ZHANG Kai¹, XIE Jun¹, LIU Qigen², YU Deguang¹, WANG Guangjun¹, YU Ermeng¹, GONG Wangbao¹, LI Zhifei¹

1 Key Laboratory of Tropical and Subtropical Fishery Resource Application and Cultivation, Ministry of Agriculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China;
2 College of Fisheries and Life Sciences, Shanghai Ocean University, Shanghai 201306, China

Abstract:

This work evaluates the application potential of a new indigenous aerobic denitrifier, strain Pseudomonas CW-2, isolated from a largemouth bass culture pond. The rate of ammonium-N removal by strain CW-2 was approximately 97% at a DO concentration of 5.2 mg/L. Furthermore, when nitrate and ammonia coexisted, the strain gave priority to assimilating ammonia, and thereafter to denitrification. Under optimal cultivation conditions, citrate and acetate were the carbon resources, C/N was 8, dissolved oxygen was 5.2 mg/L, and pH was 7; the removal rate of ammonium reached nearly 90%. The changing patterns of different bacteria in strain CW-2-treated and the control pond water were also compared. Lower levels of ammonia, nitrite, and phosphates were observed in the treated water as compared with the controls. Meanwhile, phylum-level distributions of the bacterial OTUs revealed that Proteobacteria, Bacteroidetes, Planctomycetes, and Nitrospirae continuously changed their relative abundances in relation to carbon and the addition of strain CW-2; this finding implies that the conventional denitrification process was weakened under the effects of carbon or the presence of strain CW-2. We propose that strain CW-2 is a promising organism for the removal of ammonium in intensive fish culture systems, according to our evaluations of its denitrification performance.

Key words: aerobic denitrification|ammonium removal|Micropterus salmoides|Pseudomonas CW-2

Received: 2017-03-30 Revised:

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Articles by WANG Cuicui

Articles by ZHANG Kai

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Articles by LIU Qigen

Articles by YU Deguang

Articles by WANG Guangjun

Articles by YU Ermeng

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Articles by LI Zhifei

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