Cite this paper:
LÜ Fang, DIND Gang, LIU Wei, ZHAN Dongmei, WU Haiyi, GUO Wen. Comparative study of responses in the brown algae Sargassum thunbergii to zinc and cadmium stress[J]. Journal of Oceanology and Limnology, 2018, 36(3): 933-941

Comparative study of responses in the brown algae Sargassum thunbergii to zinc and cadmium stress

LÜ Fang1,2, DIND Gang1,2, LIU Wei1,2, ZHAN Dongmei1,2, WU Haiyi1,2, GUO Wen1
1 Marine Biology Institute of Shandong Province, Qingdao 266104, China;
2 Qingdao Macroalgae Engineering Technology Research Center, Qingdao 266104, China
Abstract:
Heavy metal pollution in aquatic system is becoming a serious problem worldwide. In this study, responses of Sargassum thunbergii to different concentrations (0, 0.1, 0.5, 1.0 and 5.0 mg/L) of zinc (Zn) and cadmium (Cd) exposure separately were studied for 15 days in laboratory-controlled conditions. The results show that the specific growth rates increased slightly under the lower Zn concentration treatment (0.1 mg/L) at the first 5 d and then decreased gradually, which were significantly reduced with the exposure time in higher Zn concentrations and all Cd treatments compared to respective control, especially for 1.0 and 5.0 mg/L Cd. Chlorophyll a contents showed significant increase in 0.1 mg/L Zn treatment, whereas the gradually reduction were observed in the other three Zn treatments and all Cd treatments. The oxygen evolution rate and respiration rate presented distinct behavior in the Zn-treated samples, but both declined steadily with the exposure time in Cd treatments. The P/R value analyses showed similar variation patterns as chlorophyll a contents. Real-time PCR showed that lower Zn concentration (0.1 mg/L) increased mRNA expression of rbcL gene, whereas higher Zn concentrations and Cd reduced the rbcL expression. Taken together, these findings strongly indicate that Zn and Cd had different effects on S. thunbergii both at the physiological and gene transcription levels, the transcript level of photosynthesis-related gene rbcL can be used as an useful molecular marker of algal growth and environment impacts.
Key words:    Sargassum thunbergii|heavy metal stress|chlorophyll|photosynthesis rate|respiration rate|rbcL gene   
Received: 2017-02-24   Revised:
Tools
PDF (412 KB) Free
Print this page
Add to favorites
Email this article to others
Authors
Articles by LÜ Fang
Articles by DIND Gang
Articles by LIU Wei
Articles by ZHAN Dongmei
Articles by WU Haiyi
Articles by GUO Wen
References:
Alberte R S, Thornber J P, Fiscus E L. 1977. Water stress effects on the content and organization of chlorophyll in mesophyll and bundle sheath chloroplasts of maize. Plant Physiology, 59(3):351-353.
Amado Filho G M, Karez C S, Andrade L R, YoneshigueValentin Y, Pfeiffer W C. 1997. Effects on growth and accumulation of zinc in six seaweed species. Ecotoxicology and Environmental Safety, 37(3):223-228.
Asaolu S S, Olaofe O. 2005. Biomagnification of some heavy and essential metals in sediments, fishes and crayfish from Ondo State coastal region, Nigeria. Pakistan Journal of Scientific and Industrial Research, 48(2):96-102.
Costa G B, de Felix M R L, Simioni C, Ramlov F, Oliveira E R, Pereira D T, Maraschin M, Chow F, Horta P A, Lalau C M, da Costa C H, Matias W G, Bouzon Z L, Schmidt É C. 2016a. Effects of copper and lead exposure on the ecophysiology of the brown seaweed Sargassum cymosum. Protoplasma, 253(1):111-125.
Costa G B, Simioni C, Pereira D T, Ramlov F, Maraschin M, Chow F, Horta P A, Bouzon Z L, Schmidt É C. 2016b. The brown seaweed Sargassum cymosum:changes in metabolism and cellular organization after long-term exposure to cadmium. Protoplasma, 254(2):817-837.
Costa G B, Simioni C, Ramlov F, Maraschin M, Chow F, Bouzon Z L, Schmidt É C. 2017. Effects of manganese on the physiology and ultrastructure of Sargassum cymosum.Environmental and Experimental Botany, 133:24-34.
dos Santos R W, Schmidt É C, Martins R D P, Latini A, Maraschin M, Horta P A, Bouzon Z L. 2012. Effects of cadmium on growth, photosynthetic pigments, photosynthetic performance, biochemical parameters and structure of chloroplasts in the agarophyte Gracilaria domingensis (Rhodophyta, Gracilariales). American Journal of Plant Sciences, 3(8):1 077-1 084.
Ferreira I D, do Rosário V E, Cravo P V L. 2006. Real-time quantitative PCR with SYBR Green I detection for estimating copy numbers of nine drug resistance candidate genes in Plasmodium falciparum. Malaria Journal, 5(1):1.
Gouveia C, Kreusch M, Schmidt É C, de Felix M R, Osorio L K P, Pereira D T, dos Santos R, Ouriques L C, de Paula Martins R, Latini A, Ramlov F, Carvalho T J G, Chow F, Maraschin M, Bouzon Z L. 2013. The effects of lead and copper on the cellular architecture and metabolism of the red alga Gracilaria domingensis. Microscopy and Microanalysis, 19(3):513-524.
Hall J L. 2002. Cellular mechanisms for heavy metal detoxification and tolerance. Journal of Experimental Botany, 53(366):1-11.
Humphrey G F. 1975. The photosynthesis:respiration ratio of some unicellular marine algae. Journal of Experimental Marine Biology and Ecology, 18(2):111-119.
Jeffrey S W, Humphrey G F. 1975. New spectrophotometric equations for determining chlorophylls a, b, c1 and c2 in higher plants, algae and natural phytoplankton. Biochemie und Physiologie der Pflanzen, 167(2):191-194.
Jothinayagi N, Anbazhagan C. 2009. Heavy metal monitoring of Rameswaram coast by some Sargassum species.American-Eurasian Journal of Scientific Research, 4(2):73-80.
Lignell Å, Pedersén M. 1989. Agar composition as a function of morphology and growth rate studies on some morphological strains of Gracilaria secundata and Gracilaria verrucosa (Rhodophyta). Botanica Marina, 32(3):219-227.
Mamboya F A, Pratap H B, Mtolera M, Björk M. 1999. The effect of copper on the daily growth rate and photosynthetic efficiency of the brown macroalga Padina boergesenii. In:Richmond M D, Francis J eds. Proceedings of the Conference on Advances on Marine Sciences in Tanzania.p.185-192.
McAllister C D, Shah N, Strickland J D H. 1964. Marine phytoplankton photosynthesis as a function of light intensity:a comparison of methods. Journal of the Fisheries Research Board of Canada, 21(1):159-181.
Melis A, Melnicki M R. 2006. Integrated biological hydrogen production. International Journal of Hydrogen Energy, 31(11):1 563-1 573.
Olowu R A, Ayejuyo O O, Adewuyi G O, Adejoro I A, Denloye A A B, Babatunde A O, Ogundajo A L. 2010. Determination of heavy metals in fish tissues, water and sediment from Epe and Badagry Lagoons, Lagos, Nigeria. E-Journal of Chemistry, 7(1):215-221.
Patrón-Prado M, Casas-Valdez M, Serviere-Zaragoza E, Zenteno-Savín T, Lluch-Cota D B, Méndez-Rodríguez L. 2011. Biosorption capacity for cadmium of brown seaweed Sargassum sinicola and Sargassum lapazeanum in the Gulf of California. Water, Air, & Soil Pollution, 221:137-144.
Pichard S L, Frischer M E, Paul J H. 1993. Ribulose bisphosphate carboxylase gene expression in subtropical marine phytoplankton populations. Marine Ecology Progress Series, 101:55-65.
Ryther J H. 1955. The ratio of photosynthesis to respiration in marine plankton algae and its effect upon the measurement of productivity. Deep Sea Research, 2(2):134-139.
Saleh B. 2015. Physiological response of the green algae Ulva lactuca (chlorophyta) to heavy metals stress. Journal of Stress Physiology & Biochemistry, 11(3):38-51.
Santos R W, Schmidt É C, Vieira I C, Costa G B, Rover T, Simioni C, Barufi J B, Soares C H L, Bouzon Z L. 2015. The effect of different concentrations of copper and lead on the morphology and physiology of Hypnea musciformis cultivated in vitro:a comparative analysis. Protoplasma, 252(5):1 203-1 215.
Shao Z R, Liu F L, Li Q Y, Yao J T, Duan D L. 2014.Characterization of ribulose-1,5-bisphosphate carboxylase/oxygenase and transcriptional analysis of its related genes in Saccharina japonica (Laminariales, Phaeophyta). Chinese Journal of Oceanology and Limnology, 32(2):377-389.
Stobart A K, Griffiths W T, Ameen-Bukhari I, Sherwood R P. 1985. The effect of Cd2+ on the biosynthesis of chlorophyll in leaves of barley. Physiologia Plantarum, 63(3):293-298.
Volesky B, Holan Z R. 1995. Biosorption of heavy metals.Biotechnology Progress, 11(3):235-250.
Wu H Y, Zhan D M, Liu H J, Ding G, Liu W, Li M Z. 2010.Study on accumulation and degradation of heavy metals by the Brown alga Sargassum thunbergii. Marine Sciences, 34(1):69-74. (in Chinese with English abstract)
Xia J R, Li Y J, Lu J, Chen B. 2004. Effects of copper and cadmium on growth, photosynthesis, and pigment content in Gracilaria lemaneiformis. Bulletin of Environmental Contamination and Toxicology, 73(6):979-986.
Xu J F, Fan X, Zhang X W, Xu D, Mou S L, Cao S N, Zheng Z, Miao J L, Ye N H. 2012. Evidence of coexistence of C3 and C4 photosynthetic pathways in a green-tide-forming alga, Ulva prolifera. PLoS One, 7(5):e37438.
Copyright © Haiyang Xuebao