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ROZINA Tinkara, ELERŠEK Tina, Maja ZUPANČIČ JUSTIN, MEGLIČ Andrej, LEŠTAN Domen, SEDMAK Bojan. The effects of electrochemical oxidation on in-vivo fluorescence and toxin content in Microcystis aeruginosa culture[J]. Journal of Oceanology and Limnology, 2018, 36(4): 1091-1102 |
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The effects of electrochemical oxidation on in-vivo fluorescence and toxin content in Microcystis aeruginosa culture |
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| ROZINA Tinkara1, ELERŠEK Tina2, Maja ZUPANČIČ JUSTIN3, MEGLIČ Andrej4, LEŠTAN Domen5, SEDMAK Bojan2 |
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1 Envit d. o. o., Vojkova cesta 63, 1000 Ljubljana, Slovenia;
2 Department of Genetic Toxicology and Cancer Biology, National Institute of Biology, Večna pot 111, 1000 Ljubljana, Slovenia;
3 Arhel d. o. o., Pustovrhova ulica 15, 1000 Ljubljana, Slovenia;
4 Biotechnical Faculty, University of Ljubljana, Večna pot 111, 1000 Ljubljana;
5 Centre for Soil and Environmental Sciences, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, Slovenia |
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| Abstract: |
| The increasing occurrence of cyanobacterial blooms in water bodies is a serious threat to the environment. Efficient in-lake treatment methods for the control of cyanobacteria proliferation are needed, their in-vivo detection to obtain a real-time response to their presence, as well as the information about their physiological state after the applied treatment. In-vivo fluorescence measurements of photosynthetic pigments have proved to be effective for quantitative and qualitative detection of phytoplankton in a water environment. In the experiment, chlorophyll and phycocyanin fluorescence sensors were used concurrently to detect stress caused by electrochemical oxidation applying an electrolytic cell equipped with borondoped diamond electrodes on a laboratory culture of cyanobacteria Microcystis aeruginosa PCC 7806. The inflicted injuries were reflected in a clear transient increase in the phycocyanin fluorescence signal (for 104%±43%) 24 h after the treatment, which was not the case for the chlorophyll fluorescence signal. In the next 72 h of observation, the fluorescence signals decreased (on 40% of the starting signal) indicating a reduction of cell number, which was confirmed by cell count (24% reduction of the starting concentration) and analysis of extracted chlorophyll and phycocyanin pigment. These results demonstrate the viability of the combined application of two sensors as a useful tool for in-vivo detection of induced stress, providing real-time information needed for the evaluation of the efficiency of the in-lake treatment and decision upon the necessity of its repetition. The electrochemical treatment also resulted in a lower free microcystins concentration compared to control. |
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| Key words:
Microcystis aeruginosa|electrochemical oxidation|cyanotoxins|fluorescence|chlorophyll a|phycocyanin|boron-doped diamond electrode
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| Received: 2017-06-15 Revised: |
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