Polyphasic examination on <i>Merismopedia tenuissima</i> CHAB 7021 from Ganjiang River, China revealed the polyphyly of the genus <i>Merismopedia</i> (Cyanobacteria) -- Journal of Oceanology and Limnology,2018,36(4):1157-1165

	Cite this paper:
	SHEN Liqin, MA Suchao, CAI Fangfang, YU Gongliang, LI Shouchun, LI Renhui. Polyphasic examination on Merismopedia tenuissima CHAB 7021 from Ganjiang River, China revealed the polyphyly of the genus Merismopedia (Cyanobacteria)[J]. Journal of Oceanology and Limnology, 2018, 36(4): 1157-1165

Polyphasic examination on Merismopedia tenuissima CHAB 7021 from Ganjiang River, China revealed the polyphyly of the genus Merismopedia (Cyanobacteria)

SHEN Liqin¹, MA Suchao¹, CAI Fangfang^2,3, YU Gongliang², LI Shouchun¹, LI Renhui²

1 College of Life Science, Jiangxi Normal University, Nanchang 330022, China;
2 Key Lab of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China;
3 University of Chinese Academy of Sciences, Beijing 100049, China

Abstract:

Species in the cyanobacterial genus Merismopedia are present in freshwaters at different trophic levels, with some species even as the components of cyanobacterial blooms. However, species diversity in this genus was not fully verified by molecular investigation and polyphasic taxonomic studies. In this study, Merismopedia-like strain tenuissima CHAB 7021 was isolated from Ganjiang River in Jiangxi Province, China, and polyphasic characterization of this strain was performed by morphological observation, ultrastructural examination, chemical detection of pigments and phylogenetic analysis based on 16S rRNA gene sequences. Morphological identification of the strain was supported by the ultrastructural features, as the tiny species Merismopedia tenuissima Lemmermann. The phylogeny based on 16S rRNA gene sequences revealed at least three clades formed by the strains of Merismopedia. The three M. tenuissima strains including M. tenuissima CHAB 7021 was gathered in clade Ⅲ with distant relationship to the clade I formed by the six Merismopedia strains including the type species M. punctata, and such a genetic distance may propose Merismopedia tenuissima to separate from Merismopedia genus. However intermixture relationship in between strains of M. punctate and M. glauca in the phylogenetic tree still complicated the taxonomic status in the genus Merismopedia. The process for taxonomic revision in the Merismopedia genus still await for examination and further information on more strains of type species M. punctata.

Key words: cyanobacteria|Merismopedia|polyphasic|phylogeny|taxonomic revision

Received: 2017-11-27 Revised:

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References:
Castenholz R W, Wilmotte A, Herdman M, Rippka R, Waterbury J B, Iteman I, Hoffmann L. 2001. Phylum BX.cyanobacteria. In:Boone D R ed. Bergey's Manual^® of Systematic Bacteriology. Springer, New York. p.473-599.
Castiglioni B, Rizzi E, Frosini A, Sivonen K, Rajaniemi P, Rantala A, Mugnai M A, Ventura S, Wilmotte A, Boutte C, Grubisic S, Balthasart P, Consolandi C, Bordoni R, Mezzelani A, Battaglia C, De Bellis G. 2004. Development of a universal microarray based on the ligation detection reaction and 16S rRNA gene polymorphism to target diversity of cyanobacteria. Applied and Environmental Microbiology, 70(12):7 161-7 172.
Dantas Ê W, Moura A N, Do Carmo Bittencourt-Oliveira M. 2011. Cyanobacterial blooms in stratified and destratified eutrophic reservoirs in semi-arid region of Brazil. Anais da Academia Brasileira de Ciências, 83(4):1 327-1 338.
Desikachary T V. 1959. Cyanophyta:ICAR Monographs on Algae. Indian Council of Agricultural Research, New Delhi, Indian. 686p.
Furtado A L F F, Do Carmo Calijuri C, Lorenzi A S, Honda R Y, Genuário D B, Fiore M F. 2009. Morphological and molecular characterization of cyanobacteria from a Brazilian facultative wastewater stabilization pond and evaluation of microcystin production. Hydrobiologia, 627(1):195-209.
Geitler L. 1932. Cyanophyceae. In:Rabenhorstïs L ed.Kryptogamen Flora Von Deutschland, Österreich und der Schweiz. Akademische Verlagsgesellschaft, Leipzig, Germany. 1 196p.
Gkelis S, Rajaniemi P, Vardaka E, Moustaka-Gouni M, Lanaras T, Sivonen K. 2005. Limnothrix redekei (Van Goor)Meffert (Cyanobacteria) strains from Lake Kastoria, Greece form a separate phylogenetic group. Microbial Ecology, 49(1):176-182.
Guindon S, Dufayard J F, Lefort V, Anisimova M, Hordijk W, Gascuel O. 2010. New Algorithms and Methods to Estimate Maximum-Likelihood Phylogenies:assessing the Performance of PhyML 3.0. Systematic Biology, 59(3):307-321.
Guiry M D, Guiry G M. 2018. http://www.algaebase.org/search/genus/detail/?genus_id=V5c19ad04746d814c.Accessed on 2018-02-01.
Hall T A. 1999. BioEdit:a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41:95-98.
Hörnström E. 1999. Long-term phytoplankton changes in acid and limed lakes in SW Sweden. Hydrobiologia, 394:93-102.
Ichimura T. 1979. Media for freshwater cyanobacteria. In:Nishizawa K, Chihara M eds. Methods in Phycology.Kyouritsu Shuppan, Tokyo, Japan. p.295-296.
Joosten A M T. 2006. Flora of the Blue-Green Algae of the Netherlands. I. The Non-Filamentous Species of Inland Waters. 2^nd edn. KNNV Publishing, Utrecht, the Netherlands. 239p.
Kaas H, Koch C, Larsen J. 1985. Algal studies of the Danish Wadden Sea. Ⅲ. Blue-green algae in tidal flat sediments(sand flats and lower salt-marsh) at Rejsby:taxonomy and ecology. Opera Botanica, 79:38-61.
Komárek J, Anagnostidis K. 1986. Modern approach to the classification system of cyanophytes 2-chroococcales.Algological Studies, 43:157-226.
Komárek J, Anagnostidis K. 1999. Cyanoprokaryota 1. Teil/part I:chroococcales. In:Ettl H, Gärtner G, Heynig H, Mollenhauer D eds. Süßwasserflora von Mitteleuropa, 19/1. Spektrum Akademischer Verlag, Heidelberg, Germany. 548p.
Komárek J, Kaštovský J, Mareš J, Johansen J R. 2014.Taxonomic classification of cyanoprokaryotes(cyanobacterial genera) 2014, using a polyphasic approach. Preslia, 86(4):295-335.
Kumar S, Stecher G, Tamura K. 2016. Mega7:molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 33(7):1 870-1 874.
Lefort V, Longueville J E, Gascuel O. 2017. SMS:smart model selection in PhyML. Molecular Biology and Evolution, 34(9):2 422-2 424.
Meyen F J F. 1839. Neues system der pflanzen-physiologie.Dritter band. Haude und Spenersche Buchandlung (S.J.Joseephy), Berlin, Germany. p.1-627.
Mitchell B G. 1990. Algorithms for determining the absorption coefficient for aquatic particulates using the quantitative filter technique. In:Proceedings of SPIE 1302, Ocean Optics X. SPIE, Orlando, FL, United States. p.137-148.
Neilan B A, Jacobs D, Del Dot Therese, Blackall L L, Hawkins P R, Cox P T, Goodman A E. 1997. rRNA sequences and evolutionary relationships among toxic and nontoxic cyanobacteria of the genus microcystis. International Journal of Systematic and Evolutionary Microbiology, 47(3):693-697.
Neilan B A, Jacobs D, Goodman A E. 1995. Genetic diversity and phylogeny of toxic cyanobacteria determined by DNA polymorphisms within the phycocyanin locus.Applied and Environmental Microbiology, 61(11):3 875-3 883.
Palinska K A, Liesack W, Rhiel E, Krumbein W E. 1996.Phenotype variability of identical genotypes:the need for a combined approach in cyanobacterial taxonomy demonstrated on Merismopedia-like isolates. Archives of Microbiology, 166(4):224-233.
Posada D, Crandall K A. 1998. MODELTEST:testing the model of DNA substitution. Bioinformatics, 14(9):817-818.
Rajaniemi-Wacklin P, Rantala A, Mugnai M A, Turicchia S, Ventura S, Komárková J, Lepistö L, Sivonen K. 2006.Correspondence between phylogeny and morphology of Snowellaspp. and Woronichinianaegeliana, cyanobacteria commonly occurring in lakes. Journal of Phycology, 42(1):226-232.
Rantala A, Fewer D P, Hisbergues M, Rouhiainen L, Vaitomaa J, Börner T, Sivonen K. 2004. Phylogenetic evidence for the early evolution of microcystin synthesis. Proceedings of the National Academy of Sciences of the United States of America, 101(2):568-573.
Rippka R. 1988. Isolation and purification of cyanobacteria.Methods in Enzymology, 167:3-27.
Shao J H, Xu Y, Wang Z J, Jiang Y G, Yu G L, Peng X, Li R H. 2011. Elucidating the toxicity targets of β-ionone on photosynthetic system of Microcystis aeruginosa NIES-843 (Cyanobacteria). Aquatic Toxicology, 104(1-2):48-55.
Tamura K, Dudley J, Nei M, Kumar S. 2007. MEGA4:molecular evolutionary genetics analysis (MEGA)software version 4.0. Molecular Biology and Evolution, 24(8):1 596-1 599.
Taton A, Grubisic S, Brambilla E, De Wit R, Wilmotte A. 2003. Cyanobacterial diversity in natural and artificial microbial mats of Lake Fryxell (McMurdo Dry Valleys, Antarctica):a morphological and molecular approach.Applied and Environmental Microbiology, 69(9):5 157-5 169.
Thomazeau S, Houdan-Fourmont A, Couté A, Duval C, Couloux A, Rousseau F, Bernard C. 2010. The contribution of sub-saharan African strains to the phylogeny of Cyanobacteria:focusing on the Nostocaceae (Nostocales, Cyanobacteria). Journal of Phycology, 46(3):564-579.
Zhu H R. 1991. Flora Algarum Sinicarum Aquae Dulcis Tomus Ⅱ. Chroococcophyceae. Science Press, Beijing, China. 161p. (in Chinese)