Comparative study of the hemolytic and cytotoxic activities of nematocyst venoms from the jellyfish <i>Cyanea nozakii</i> Kishinouye and <i>Nemopilema nomurai</i> Kishinouye -- Journal of Oceanology and Limnology,2018,36(4):1255-1265

	Cite this paper:
	PANG Min, XU Jintao, LIU Yunlong, ZHANG Xuelei. Comparative study of the hemolytic and cytotoxic activities of nematocyst venoms from the jellyfish Cyanea nozakii Kishinouye and Nemopilema nomurai Kishinouye[J]. Journal of Oceanology and Limnology, 2018, 36(4): 1255-1265

Comparative study of the hemolytic and cytotoxic activities of nematocyst venoms from the jellyfish Cyanea nozakii Kishinouye and Nemopilema nomurai Kishinouye

PANG Min^1,2, XU Jintao³, LIU Yunlong⁴, ZHANG Xuelei^1,2

1. Key Laboratary of Science and Engineering for Marine Ecology and Environment, First Institute of Oceanography, State Oceanic Administration, Qingdao 266003, China;
2 Laboratory of Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266200, China;
3 Qinhuangdao Marine Environmental Monitoring Central Station of SOA, Qinhuangdao 066000, China;
4 Chinese Research Academy of Environmental Sciences, Beijing 100012, China

Abstract:

Two species of jellyfish, Cyanea nozakii Kishinouye and Nemopilema nomurai Kishinouye, have occurred off coastal areas of the northeastern China Sea, Yellow Sea, and Bohai Sea in recent years. They influence marine ecosystem safety and fishery production, and also pose a risk to human health. The current study examined the hemolytic and cytotoxic activities of crude venoms extracted from the nematocysts of C. nozakii and N. nomurai. The results showed that there were more nematocysts on tentacles from C. nozakii than on tentacles of the same length from N. nomurai. The protein concentration per nematocyst extracted from N. nomurai was higher than that from C. nozakii. Both nematocyst venoms showed dose- and timedependent hemolytic activity on erythrocytes from chicken, pigeon, and sheep, with sheep erythrocytes being the most sensitive, with EC₅₀ values of 69.69 and 63.62 μg/mL over a 30-min exposure with N. nomurai and C. nozakii nematocyst venoms, respectively. A cytotoxic assay of both jellyfish venoms on A431 human epidermal carcinoma cells resulted in IC₅₀ values of 68.6 and 40.9 μg/mL after 24-h incubation, respectively, with venom from C. nozakii showing stronger cytotoxic activity than that from N. nomurai. The results of current study indicate that nematocyst venom from C. nozakii had stronger hemolytic and cytotoxic activities than that from N. nomurai and, thus, C. nozakii might be more harmful to the health of humans and other species than are N. nomurai when they appear in coastal waters.

Received: 2017-03-30 Revised:

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