A potential germ cell-specific marker in Japanese flounder, <i>Paralichthys olivaceus</i>: identification and characterization of lymphocyte antigen 75 (Ly75/CD205) -- Journal of Oceanology and Limnology,2018,36(4):1342-1348

	Cite this paper:
	YANG Yang, LIU Qinghua, MA Daoyuan, SONG Zongcheng, LI Jun. A potential germ cell-specific marker in Japanese flounder, Paralichthys olivaceus: identification and characterization of lymphocyte antigen 75 (Ly75/CD205)[J]. Journal of Oceanology and Limnology, 2018, 36(4): 1342-1348

A potential germ cell-specific marker in Japanese flounder, Paralichthys olivaceus: identification and characterization of lymphocyte antigen 75 (Ly75/CD205)

YANG Yang^1,2,3, LIU Qinghua^1,2, MA Daoyuan^1,2, SONG Zongcheng⁴, LI Jun^1,2

1 Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;
2 Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China;
3 University of Chinese Academy of Sciences, Beijing 100049, China;
4 Weihai Shenghang Aquatic Product Science and Technology Co. Ltd., Weihai 264200, China

Abstract:

Some germ cell marker genes, such as vasa, nanos, and dead end (dnd), have been identified in fish. Recently, lymphocyte antigen 75 (Ly75/CD205) has been identified as a mitotic germ cell-specific cell-surface marker in several fish species. In this study, the Japanese flounder Paralichthys olivaceus ly75 homolog (ly75) was cloned and its expression pattern in gonads was analyzed. The full-length cDNA of ly75 was 7 346 bp, with an open reading frame (ORF) of 5 229 bp. The ORF encoded a protein containing 1 742 amino acids with a predicted molecular mass of 196.89 kDa. In adult tissues, ly75 transcripts were detected in all analyzed tissues but abundantly in the testis. In in-situ hybridization analyses, ly75 mRNA was predominantly localized in oocytes in the ovary and spermatogonia in the testis, but ly75 mRNA was not detected in oogonia, spermatocytes, spermatids, or spermatozoa. These results indicated that ly75 could be a potential germ cell-specific marker in P. olivaceus, as in other fishes.

Key words: Ly75/CD205|Japanese flounder|Paralichthys olivaceus|gonads|germ cell-specific marker

Received: 2017-12-19 Revised:

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Articles by YANG Yang

Articles by LIU Qinghua

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