De novo transcriptome sequencing reveals candidate genes involved in orange shell coloration of bay scallop <i>Argopecten irradians</i> -- Journal of Oceanology and Limnology,2018,36(4):1408-1416

	Cite this paper:
	TENG Wen, CONG Rihao, QUE Huayong, ZHANG Guofan. De novo transcriptome sequencing reveals candidate genes involved in orange shell coloration of bay scallop Argopecten irradians[J]. Journal of Oceanology and Limnology, 2018, 36(4): 1408-1416

De novo transcriptome sequencing reveals candidate genes involved in orange shell coloration of bay scallop Argopecten irradians

TENG Wen^1,2, CONG Rihao^1,3,4, QUE Huayong^1,3,4, ZHANG Guofan^1,3,4

1 Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;
2 University of Chinese Academy of Sciences, Beijing 100049, China;
3 Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China;
4 National & Local Joint Engineering Laboratory of Ecological Mariculture, Qingdao 266000, China

Abstract:

Molluscan shell color has received persistent attention for its distinctive diversity and complexity. In the present study, six transcriptome libraries obtained from two developmental stages, pre-pigmentation and post-pigmentation, were used for paired-end sequencing in the bay scallop Argopecten irradians. In total, 289 839 646 paired-end reads were assembled into 70 929 transcripts. Using BLASTX and BLASTN, 30 896 unigenes were successfully annotated against the SWISS-PROT, NR, and KOG database. Gene ontology annotation and Kyoto Encyclopedia of Genes and Genomes classification identified numbers of unigenes involved in biomineralization and pigmentation. Digital gene expression analysis revealed that melanin, trace metal elements and porphyrins are potentially involved in shell coloration of A. irradians.

Key words: molluscs|shell coloration|differentially expressed genes|gene ontology

Received: 2017-03-03 Revised:

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