Cite this paper:
Oscar Omondi DONDE, TIAN Cuicui, XIAO Bangding. Integrated site-specific quantification of faecal bacteria and detection of DNA markers in faecal contamination source tracking as a microbial risk tracking tool in urban Lake ecosystems[J]. HaiyangYuHuZhao, 2018, 36(5): 1629-1642

Integrated site-specific quantification of faecal bacteria and detection of DNA markers in faecal contamination source tracking as a microbial risk tracking tool in urban Lake ecosystems

Oscar Omondi DONDE1,2,3, TIAN Cuicui1, XIAO Bangding1
1 Key Laboratory of Algal Biology of Chinese Academy of Sciences-Lake Restoration Research Group, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China;
2 International College, University of Chinese Academy of Sciences, Beijing 100049, China;
3 Egerton University, Department of Environmental Science, P. O. Box 536-20115, Egerton-Kenya
Abstract:
The presence of feacal-derived pathogens in water is responsible for several infectious diseases and deaths worldwide. As a solution, sources of fecal pollution in waters must be accurately assessed, properly determined and strictly controlled. However, the exercise has remained challenging due to the existing overlapping characteristics by different members of faecal coliform bacteria and the inadequacy of information pertaining to the contribution of seasonality and weather condition on tracking the possible sources of pollution. There are continued efforts to improve the Faecal Contamination Source Tracking (FCST) techniques such as Microbial Source Tracking (MST). This study aimed to make contribution to MST by evaluating the efficacy of combining site specific quantification of faecal contamination indicator bacteria and detection of DNA markers while accounting for seasonality and weather conditions' effects in tracking the major sources of faecal contamination in a freshwater system (Donghu Lake, China). The results showed that the use of cyd gene in addition to lacZ and uidA genes differentiates E. coli from other closely related faecal bacteria. The use of selective media increases the pollution source tracking accuracy. BSA addition boosts PCR detection and increases FCST efficiency. Seasonality and weather variability also influence the detection limit for DNA markers.
Key words:    assay|contamination|faecal bacteria indicator|source tracking|water quality   
Received: 2017-04-07   Revised:
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Articles by Oscar Omondi DONDE
Articles by TIAN Cuicui
Articles by XIAO Bangding
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