Cite this paper:
YANG Xiaoqian, WEN Xin, ZHOU Chengxu, ZHU Xiaojuan, MENG Ran, LUO Qijun, YAN Xiaojun. Comparative study of brine shrimp bioassay-based toxic activities of three harmful microalgal species that frequently blooming in aquaculture ponds[J]. HaiyangYuHuZhao, 2018, 36(5): 1697-1706

Comparative study of brine shrimp bioassay-based toxic activities of three harmful microalgal species that frequently blooming in aquaculture ponds

YANG Xiaoqian1, WEN Xin1,4, ZHOU Chengxu1,2, ZHU Xiaojuan1, MENG Ran3, LUO Qijun1,2, YAN Xiaojun1,2,3
1 School of Marine Science, Ningbo University, Ningbo 315211, China;
2 Key Laboratory of Marine Biotechnology of Zhejiang Province, Ningbo University, Ningbo 315211, China;
3 Li Dak Sum Yip Yio Chin Kenneth Li Marine Biopharmaceutical Research Center, Ningbo University, Ningbo 315211, China;
4 Senior High School of Yudu, Ganzhou 343200, China
Abstract:
Typical harmful micro-algal species constantly occurred in high density in marine aquaculture ponds in Xiangshan and Sanmen Bay, Zhejiang Province. Fates of the microalgal cells influenced by activity of the cultured animals largely determined the effects of the harmful microalgae. However, it is difficult to detect the in situ process. In this paper, toxic activities of three harmful microalga, namely Prymnesium parvum, Pleurochrysis elongata, Karlodinium veneficum, which were isolated from the local ponds, were comparatively studied based on brine shrimp toxic bioassays. Different lethal activities of live cells, cell debris, cellular extracts, and cell free mediums prepared by different process were analyzed. The results showed that, (1) all of the three microalgal species had density and time dependent lethal effects on Artermia nauplii, while P. parvum was the most toxic one and had acute lethal effects in 5 h. No such acute lethal effects were observed in P. elongata or K. veneficum; (2) live cells, cell debris and cellular extracts of P. parvum had the same lethal pattern. Prymnesins, toxin from P. parvum, is probably not exotoxic active; For P. elongata, toxic activity mainly came from live cells and cell debris; For K. veneficum, toxic activity was relatively lower compared with the other two species. However, Karlotoxin, toxin from K. veneficum, is exotoxic active. Physical disturbance triggered K. veneficum cells actively releasing toxins, which made it an active predator.
Key words:    Prymnesium parvum|Pleurochrysis elongata|Karlodinium veneficum|Artemia nauplii|toxic activity   
Received: 2017-05-10   Revised:
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Articles by YANG Xiaoqian
Articles by WEN Xin
Articles by ZHOU Chengxu
Articles by ZHU Xiaojuan
Articles by MENG Ran
Articles by LUO Qijun
Articles by YAN Xiaojun
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