Cite this paper:
LIU Jun, XU Fei, JI Peng, LI Li, ZHANG Guofan. Involvement of clustered oyster Wnt genes in gut formation[J]. HaiyangYuHuZhao, 2018, 36(5): 1746-1752

Involvement of clustered oyster Wnt genes in gut formation

LIU Jun1,2,4, XU Fei1,3,4, JI Peng1,2,4, LI Li1,4,5, ZHANG Guofan1,3,4
1 Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;
2 University of Chinese Academy of Sciences, Beijing 100049, China;
3 Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266000, China;
4 National & Local Joint Engineering Laboratory of Ecological Mariculture, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;
5 Laboratory for Marine Fisheries and Aquaculture, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266000, China
Abstract:
Genes encoding Wnt ligands play important roles in organ development. The Wnt10-Wnt6-Wnt1-Wnt9 cluster widely presents in many metazoan genomes, indicating the importance of gene arrangement. Hypothesis has been proposed that they may be coordinately regulated. However, few expression correlations were identified in model animals. We analyzed the tissue expression pattern of clustered oyster Wnt10, Wnt6, Wnt1, and Wnt9a genes in this study. The results indicated the highest expression level in adult gut system of these clustered Wnt genes, except for Wnt6, which had highest expression in mantle. Further whole-mount immunofluorescence assay indicated that Wnt6 protein was restricted to gut region in oyster larvae. These results suggest the possible important role of the Wnt10-Wnt6-Wnt1-Wnt9 cluster in oyster gut formation.
Key words:    gene cluster|tissue distribution|digestive gland|immunofluorescence|Crassostrea gigas   
Received: 2017-04-28   Revised:
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References:
Adamska M, Degnan S M, Green K M, Adamski M, Craigie A, Larroux C, Degnan B M, Fraser J. 2007. Wnt and TGF-β expression in the sponge Amphimedon queenslandica and the origin of metazoan embryonic patterning. PLoS One, 2(10):e1031, https://doi.org/10.1371/journal.pone.0001031.
Adamska M, Larroux C, Adamski M, Green K, Lovas E, Koop D, Richards G S, Zwafink C, Degnan B M. 2010. Structure and expression of conserved Wnt pathway components in the demosponge Amphimedon queenslandica. Evolution & Development, 12(5):494-518, https://doi.org/10.1111/j.1525-142X.2010.00435.x.
Bao Y B, Xu F, Shimeld S M. 2017. Phylogenetics of lophotrochozoan bHLH genes and the evolution of lineage-specific gene duplicates. Genome Biology and Evolution, 9(4):869-886, https://doi.org/10.1093/gbe/evx047.
Bodine P V N. 2008. Wnt signaling control of bone cell apoptosis. Cell Research, 18(2):248-253, https://doi.org/10.1038/cr.2008.13.
Bolognesi R, Beermann A, Farzana L, Wittkopp N, Lutz R, Balavoine G, Brown S J, Schröder R. 2008. Tribolium Wnts:evidence for a larger repertoire in insects with overlapping expression patterns that suggest multiple redundant functions in embryogenesis. Development Genes and Evolution, 218(3-4):193-202, https://doi.org/10.1007/s00427-007-0170-3.
Brooke N M, Garcia-Fernàndez J, Holland P W H. 1998. The ParaHox gene cluster is an evolutionary sister of the Hox gene cluster. Nature, 392(6679):920-922, https://doi.org/10.1038/31933.
Cho S J, Vallès Y, Giani V C, Seaver E C, Weisblat D A. 2010. Evolutionary dynamics of the wnt gene family:a lophotrochozoan perspective. Molecular Biology and Evolution, 27(7):1 645-1 658, https://doi.org/10.1093/molbev/msq052.
Cohen E D, Mariol M C, Wallace R M H, Weyers J, Kamberov Y G, Pradel J, Wilder E L. 2002. DWnt4 regulates cell movement and focal adhesion kinase during Drosophila ovarian morphogenesis. Developmental Cell, 2(4):437-448, https://doi.org/10.1016/S1534-5807(02)00142-9.
Cordero J B, Ridgway R A, Valeri N, Nixon C, Frame M C, Muller W J, Vidal M, Sansom O J. 2014. c-Src drives intestinal regeneration and transformation. EMBO Journal, 33(13):1 474-1 491, https://doi.org/10.1002/embj.201387454.
Croce J, Range R, Wu S Y, Miranda E, Lhomond G, Peng J C F, Lepage T, McClay D R. 2011. Wnt6 activates endoderm in the sea urchin gene regulatory network. Development, 138(15):3 297-3 306, https://doi.or