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LIU Jun, XU Fei, JI Peng, LI Li, ZHANG Guofan. Involvement of clustered oyster Wnt genes in gut formation[J]. HaiyangYuHuZhao, 2018, 36(5): 1746-1752

Involvement of clustered oyster Wnt genes in gut formation

LIU Jun1,2,4, XU Fei1,3,4, JI Peng1,2,4, LI Li1,4,5, ZHANG Guofan1,3,4
1 Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;
2 University of Chinese Academy of Sciences, Beijing 100049, China;
3 Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266000, China;
4 National & Local Joint Engineering Laboratory of Ecological Mariculture, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;
5 Laboratory for Marine Fisheries and Aquaculture, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266000, China
Genes encoding Wnt ligands play important roles in organ development. The Wnt10-Wnt6-Wnt1-Wnt9 cluster widely presents in many metazoan genomes, indicating the importance of gene arrangement. Hypothesis has been proposed that they may be coordinately regulated. However, few expression correlations were identified in model animals. We analyzed the tissue expression pattern of clustered oyster Wnt10, Wnt6, Wnt1, and Wnt9a genes in this study. The results indicated the highest expression level in adult gut system of these clustered Wnt genes, except for Wnt6, which had highest expression in mantle. Further whole-mount immunofluorescence assay indicated that Wnt6 protein was restricted to gut region in oyster larvae. These results suggest the possible important role of the Wnt10-Wnt6-Wnt1-Wnt9 cluster in oyster gut formation.
Key words:    gene cluster|tissue distribution|digestive gland|immunofluorescence|Crassostrea gigas   
Received: 2017-04-28   Revised:
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