Characterization, tissue distribution, and expression of neuropeptide Y in olive flounder <i>Paralichthys olivaceus</i> -- Journal of Oceanology and Limnology,2015,33(3):553-558

	Cite this paper:
	WANG Qian, TAN Xungang, DU Shaojun, SUN Wei, YOU Feng, ZHANG Peijun. Characterization, tissue distribution, and expression of neuropeptide Y in olive flounder Paralichthys olivaceus[J]. Journal of Oceanology and Limnology, 2015, 33(3): 553-558

Characterization, tissue distribution, and expression of neuropeptide Y in olive flounder Paralichthys olivaceus

WANG Qian^1,2, TAN Xungang¹, DU Shaojun³, SUN Wei¹, YOU Feng¹, ZHANG Peijun¹

1 Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;
2 University of Chinese Academy of Sciences, Beijing 100049, China;
3 Department of Biochemistry and Molecular Biology, School of Medicine, University of Maryland 701 E. Pratt St, Baltimore MD, 21202, USA

Abstract:

Neuropeptide Y (NPY) is a 36-amino acid peptide of the neuropeptide Y family that plays key roles in the regulation of food intake. In this study, we focused on NPY mRNA expression changes around feeding time and during food deprivation in olive flounder. The olive flounder NPY mRNA levels were analyzed in different tissues and a high level of expression was detected in the brain. We also demonstrated a correlation between NPY expression levels in the brain and feeding schedule. NPY expression levels in olive flounder maintained on a daily scheduled feeding regimen increased shortly before feeding and decreased after the scheduled feeding time. Compared with the -1 h group before feeding, NPY expression in the 3 h group after feeding decreased significantly (P <0.05). Food deprivation led to an 81.7% decrease in NPY mRNA levels in the 24 h fasted group (P <0.05) and a 91.7% decrease in the 48 h fasted group (P <0.05). Therefore, our study demonstrates that NPY expression is associated with food intake in olive flounder. This result reveals the function of NPY in regulating food intake and its potential importance in olive flounder aquaculture.

Received: 2014-04-04 Revised: 2014-11-04

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Articles by WANG Qian

Articles by TAN Xungang

Articles by DU Shaojun

Articles by SUN Wei

Articles by YOU Feng

Articles by ZHANG Peijun

References:
Aldegunde M, Mancebo M. 2006. Effects of neuropeptide Y on food intake and brain biogenic amines in the rainbow trout (Oncorhynchus mykiss). Peptides, 27 (4): 719-727.
Campos V F, Collares T, Deschamps J C, Seixas F K,Dellagostin O A, Lanes C F C, Sandrini J, Marins L F,Okamoto M, Sampaio L A, Robaldo R B. 2010.Identification, tissue distribution and evaluation of brain neuropeptide Y gene expression in the Brazilian flounder Paralichthys orbignyanus. J. Biosci., 35 (3): 405-413.
Carpio Y, Acosta J, Morales A, Herrera F, Gonzalez L J,Estrada M P. 2006. Cloning, expression and growth promoting action of Red tilapia (Oreochromis sp.) neuropeptide Y. Peptides, 27 (4): 710-718.
Cerda-Reverter J M, Martinez-Rodriguez G, Zanuy S, Carrillo M, Larhammar D. 2000. Molecular evolution of the neuropeptide Y (NPY) family of peptides: cloning of three NPY-related peptides from the sea bass (Dicentrarchus labrax). Regul. Pept., 95 (1-3): 25-34.
Cerda-Reverter J M, Sorbera L A, Carrillo M, Zanuy S. 1999.Energetic dependence of NPY-induced LH secretion in a teleost fish (Dicentrarchus labrax). Am. J. Physiol. Regul.Integr. Comp. Physiol., 277 (6): R1627-R1634.
Drew R E, Rodnick K J, Settles M, Wacyk J, Churchill E,Powell M S, Hardy R W, Murdoch G K, Hill R A, Robison B D. 2008. Effect of starvation on transcriptomes of brain and liver in adult female zebrafish (Danio rerio). Physiol.Genomics, 35 (3): 283-295.
Dumont Y, Martel J C, Fournier A, Stpierre S, Quirion R. 1992.Neuropeptide-Y and neuropeptide-Y receptor subtypes in brain and peripheral-tissues. Prog. Neurobiol., 38 (2): 125-167.
Jones D T, Taylor W R, Thornton J M. 1992. The rapid generation of mutation data matrices from protein sequences. Comput. Appl. Biosci., 8 : 275-282.
Kehoe A S, Volkoff H. 2007. Cloning and characterization of neuropeptide Y (NPY) and cocaine and amphetamine regulated transcript (CART) in Atlantic cod (Gadus morhua). Comp. Biochem. Physiol. A-Mol. Integr.Physiol., 146 (3): 451-461.
Kiris G A, Kumlu M, Dikel S. 2007. Stimulatory effects of neuropeptide Y on food intake and growth of Oreochromis niloticus. Aquaculture, 264 (1-4): 383-389.
Kurokawa T, Suzuki T. 2002. Development of neuropeptide Y-related peptides in the digestive organs during the larval stage of Japanese flounder, Paralichthys olivaceus. Gen.Comp. Endocrinol., 126 (1): 30-38.
Larhammar D. 1996. Evolution of neuropeptide Y, peptide YY and pancreatic polypeptide. Regul. Pept., 62 (1): 1-11.
Livak K J, Schmittgen T D. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2^-ΔΔCt method. Method s, 25 (4): 402-408.
Lopez-Patino M A, Guijarro A I, Isorna E, Delgado M J,Alonso-Bedate M, de Pedro N. 1999. Neuropeptide Y has a stimulatory action on feeding behavior in goldfish (Carassius auratus). Eur. J. Pharmacol., 377 (2-3): 147-153.
MacDonald E, Volkoff H. 2009. Neuropeptide Y (NPY), cocaine-and amphetamine-regulated transcript (CART) and cholecystokinin (CCK) in winter skate (Raja ocellata): cDNA cloning, tissue distribution and mRNA expression responses to fasting. Gen. Comp. Endocrinol., 161 (2): 252-261.
Murashita K, Kurokawa T, Ebbesson L O E, Stefansson S O,Ronnestad I. 2009. Characterization, tissue distribution, and regulation of agouti-related protein (AgRP), cocaineand amphetamine-regulated transcript (CART) and neuropeptide Y (NPY) in Atlantic salmon (Salmo salar).Gen. Comp. Endocrinol., 162 (2): 160-171.
Narnaware Y K, Peyon P P, Lin X W, Peter R E. 2000.Regulation of food intake by neuropeptide Y in goldfish.Am. J. Physiol.-Regul. Integr. Comp. Physiol., 279 (3):R1025-R1034.
Peng C, Chang J P, Yu K L, Wong A O L, Vangoor F, Peter R E, Rivier J E. 1993. Neuropeptide-Y stimulates growthhormone and gonadotropin-ii secretion in the goldfish pituitary: involvement of both presynaptic and pituitary cell actions. Endocrinology, 132 (4): 1 820-1 829.
Silverstein J T, Plisetskaya E M. 2000. The effects of NPY and insulin on food intake regulation in fish. Am. Zool., 40 (2): 296-308.
Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. 2011. MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol. Biol.Evol., 28 (10): 2 731-2 739.
Tang Z G, Sun C Y, Yan A F, Wu S G, Qin C B, Zhang Y H, Li W S. 2013. Genes involved in fatty acid metabolism: molecular characterization and hypothalamic mRNA response to energy status and neuropeptide Y treatment in the orange-spotted grouper Epinephelus coioides. Mol Cell Endocrinol., 376 (1-2): 114-124.
Tatemoto K. 1982. Neuropeptide-Y: complete amino-acidsequence of the brain peptide. Proc. Natl. Acad. Sci. USA, 79 (18): 5 485-5 489.
Yokobori E, Azuma M, Nishiguchi R, Kang K S, Kamijo M,Uchiyama M, Matsuda K. 2012. Neuropeptide Y stimulates food intake in the zebrafish, Danio rerio. J.Neuroendocrinol., 24 (5): 766-773.
Zhang L, Nguyen A D, Lee I-C J, Yulyaningsih E, Riepler S J,Stehrer B, Enriquez R F, Lin S, Shi Y-C, Baldock P A,Sainsbury A, Herzog H. 2012. NPY modulates PYY function in the regulation of energy balance and glucose homeostasis. Diabetes Obes Metab., 14 (8): 727-736.