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XIA Mengjie, PEI Feng, MU Changkao, YE Yangfang, WANG Chunlin. Disruption of bacterial balance in the gut of Portunus trituberculatus induced by Vibrio alginolyticus infection[J]. HaiyangYuHuZhao, 2018, 36(5): 1891-1898

Disruption of bacterial balance in the gut of Portunus trituberculatus induced by Vibrio alginolyticus infection

XIA Mengjie1,2, PEI Feng1, MU Changkao1, YE Yangfang1, WANG Chunlin1
1 Key Laboratory of Applied Marine Biotechnology(Ningbo University), Ministry of Education, Ningbo 315211, China;
2 Collaborative Innovation Center for Zhejiang Marine High-Efficiency and Healthy Aquaculture, Ningbo 315211, China
Gut microbiota impacts the health of crustaceans. Vibrio alginolyticus is a main causative pathogen that induces the vibriosis in farmed swimming crabs, Portunus trituberculatus. However, it remains unknown whether gut bacteria perform functions during the progression of vibriosis. In this study, 16S rRNA gene amplicon sequencing was used to investigate temporal alteration of gut bacterial community in swimming crabs in response to 72-h V. alginolyticus challenge. Our results show that V. alginolyticus infection resulted in dynamic changes of bacterial community composition in swimming crabs. Such changes were highlighted by the overwhelming overabundance of Vibrio and a significant fluctuation in the gut bacteria including the bacteria with high relative abundance and especially those with low relative abundance. These findings reveal that crab vibriosis gradually develops with the infection time of V. alginolyticus and tightly relates to the dysbiosis of gut bacterial community structure. This work contributes to our appreciation of the importance of the balance of gut bacterial community structure in maintaining the health of crustaceans.
Key words:    Portunus trituberculatus|Vibrio alginolyticus|gut bacterial community composition|16S rRNA gene amplicon sequencing   
Received: 2017-06-02   Revised:
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Bi K R, Zhang X J, Yan B L, Gao H, Gao X J, Sun J J. 2016.Isolation and molecular identification of Vibrio natriegens from diseased Portunus trituberculatus in China. Journal of the World Aquaculture Society, 47(6):854-861.
Brestoff J R, Artis D. 2013. Commensal bacteria at the interface of host metabolism and the immune system. Nature Immunology, 14(7):676-684.
Caporaso J G, Bittinger K, Bushman F D, DeSantis T Z, Andersen G L, Knight R. 2010b. PyNAST:a flexible tool for aligning sequences to a template alignment.Bioinformatics, 26(2):266-267.
Caporaso J G, Kuczynski J, Stombaugh J, Bittinger K, Bushman F D, Costello E K, Fierer N, Peña A G, Goodrich J K, Gordon J I, Huttley G A, Kelley S T, Knights D, Koenig J E, Ley R E, Lozupone C A, McDonald D, Muegge B D, Pirrung M, Reeder J, Sevinsky J R, Turnbaugh P J, Walters W A, Widmann J, Yatsunenko T, Zaneveld J, Knight R. 2010a. QⅡME allows analysis of high-throughput community sequencing data. Nature Methods, 7(5):335-336.
Caporaso J G, Lauber C L, Walters W A, Berg-Lyons D, Huntley J, Fierer N, Owens S M, Betley J, Fraser L, Bauer M, Gormley N, Gilbert J A, Smith G, Knight R. 2012.Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms. The ISME Journal, 6(8):1 621-1 624.
Chen X B, Di P P, Wang H M, Li B L, Pan Y J, Yan S L, Wang Y J. 2015. Bacterial community associated with the intestinal tract of Chinese mitten crab (Eriocheir sinensis)farmed in Lake Tai, China. PLoS One, 10(4):e0123990.
Cheung M K, Yip H Y, Nong W, Law P T W, Chu K H, Kwan H S, Hui J H L. 2015. Rapid change of microbiota diversity in the gut but not the hepatopancreas during gonadal development of the new shrimp model Neocaridina denticulata. Marine Biotechnology, 17(6):811-819.
Cui Z X, Liu Y, Luan W S, Li Q Q, Wu D H, Wang S Y. 2010.Molecular cloning and characterization of a heat shock protein 70 gene in swimming crab (Portunus trituberculatus). Fish & Shellfish Immunology, 28(1):56-64.
De Schryver P, Vadstein O. 2014. Ecological th