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Cite this paper: |
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MENG Wei, YANG Tianyan, HAI Sa, MA Yanwu, CAI Lingang, MA Xufa, GAO Tianxiang, GUO Yan. Extensive genetic divergence among Diptychus maculatus populations in northwest China[J]. Journal of Oceanology and Limnology, 2015, 33(3): 577-584 |
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Extensive genetic divergence among Diptychus maculatus populations in northwest China |
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| MENG Wei1, YANG Tianyan1,2, HAI Sa1, MA Yanwu1, CAI Lingang1, MA Xufa3, GAO Tianxiang2, GUO Yan1 |
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1 Xinjiang Fishery Research Institute, Urumqi 830000, China;
2 Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao 266003, China;
3 Fisheries College of Huazhong Agricultural University, Wuhan 430070, China |
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| Abstract: |
| D. maculates is a kind of specialized Schizothoracinae fish has been locally listed as a protected animal in Xinjiang Province, China. Ili River located in north of Tianshan Mountain and Tarim River located in north of Qinghai-Tibetan Plateau were two main distribution areas of this fish. To investigate the genetic diversity and genetic structure of D. maculates, four populations from Tarim River system and two populations from Ili River system were collected in this study. A 570-bp sequence of the control region was obtained for 105 specimens. Twenty-four haplotypes were detected from six populations, only Kunes River population and Kashi River population shared haplotypes with each other. For all the populations examined, the haplotype diversity (h) was 0.904 8±0.012 6, nucleotide diversity (π) was 0.027 9±0.013 9, and the average number of pairwise nucleotide differences (k) was 15.878 3±7.139 1. The analysis of molecular variance (AMOVA) showed that 86.31% of the total genetic variation was apportioned among populations, and the variation within sampled populations was 13.69%. Genetic differences among sampled populations were highly significant. Fst statistical test indicated that all populations were significantly divergent from each other (P <0.01). The largest Fst value was between Yurungkash River population and Muzat River population, while the smallest Fst value was between Kunes River population and Kashi River population. NJ phylogenetic tree of D-loop haplotypes revealed two main clades. The neutrality test and mismatch distribution analysis suggested that the fish had went through a recent population expansion. The uplift of Tianshan Mountain and movement of Qinghai-Tibetan Plateau might contribute to the wide genetic divergence of D. maculates in northwest China. |
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| Key words:
D. maculates|Tarim River system|Ili River system|genetic divergence|genetic structure
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| Received: 2014-06-06 Revised: 2014-10-23 |
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