A re-investigation of the bloom-forming unarmored dinoflagellate <i>Karenia longicanalis</i> (syn. <i>Karenia umbella</i>) from Chinese coastal waters -- Journal of Oceanology and Limnology,2018,36(6):2202-2215

	Cite this paper:
	WANG Jianyan, CEN Jingyi, LI Si, LÜ Songhui, MOESTRUP Øjvind, CHAN Kin-Ka, JIANG Tao, LEI Xiangdong. A re-investigation of the bloom-forming unarmored dinoflagellate Karenia longicanalis (syn. Karenia umbella) from Chinese coastal waters[J]. Journal of Oceanology and Limnology, 2018, 36(6): 2202-2215

A re-investigation of the bloom-forming unarmored dinoflagellate Karenia longicanalis (syn. Karenia umbella) from Chinese coastal waters

WANG Jianyan¹, CEN Jingyi^2,3, LI Si^2,3, LÜ Songhui^2,3, MOESTRUP Øjvind⁴, CHAN Kin-Ka⁵, JIANG Tao⁶, LEI Xiangdong⁷

1 Department of Science Research, Beijing Museum of Natural History, Beijing 100050, China;
2 College of Life Science and Technology, Jinan University, Guangzhou 510632, China;
3 Key Laboratory of Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes, Jinan University, Guangzhou 510632, China;
4 Marine Biological Section, Department of Biology, University of Copenhagen, Universitetsparken 4, DK-2100 Copenhagen Ø, Denmark;
5 School of Science & Technology, Open University of Hong Kong, 999077 Kowloon, Hong Kong, China;
6 Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;
7 Wenzhou Marine Environmental Monitoring Center Station, State Oceanic Administration, Wenzhou 325013, China

Abstract:

The dinoflagellate genus Karenia is known for recurrent harmful blooms worldwide. However, species diversity of the genus is generally overlooked owing to the difficulty of identifying small unarmored dinoflagellates. We have established four clonal cultures of Karenia longicanalis isolated from the type locality, Hong Kong harbor (strain HK01) and other three locations along the Chinese coasts (strains YB01, DT01, and NJ01). The morphology of the strain was studied by light and scanning electron microscopy (LM and SEM) and the pigment composition analyzed by high-performance liquid chromatography. We provide the first molecular data of K. longicanalis based on the large subunit (LSU) rRNA gene sequence and internal transcribed spacer (ITS). The four strains showed identical LSU rDNA sequences with a similarity of 99.4% to the holotype of Karenia umbella (strain KUTN05) from Australia. In the ITS phylogeny, the sequence of K. umbella branched between the Chinese strains of K. longicanalis. A careful comparison of the morphology of K. longicanalis and K. umbella reveals the similarity in the diagnostic characters. Differences may appear due to the sample treatment for SEM. We conclude that K. umbella is a junior synonym of K. longicanalis.

Received: 2017-06-28 Revised:

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Articles by WANG Jianyan

Articles by CEN Jingyi

Articles by LI Si

Articles by LÜ Songhui

Articles by MOESTRUP Øjvind

Articles by CHAN Kin-Ka

Articles by JIANG Tao

Articles by LEI Xiangdong

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