Cite this paper:
SUN Hushan, WANGYiyan, LIU Xiaoli, LIU Dongwu. Immunohistochemical identification of dynorphin A and Kappa opioid receptor-1 in the digestive system of scallop Chlamys farreri[J]. HaiyangYuHuZhao, 2018, 36(6): 2288-2296

Immunohistochemical identification of dynorphin A and Kappa opioid receptor-1 in the digestive system of scallop Chlamys farreri

SUN Hushan1, WANGYiyan1, LIU Xiaoli1, LIU Dongwu2
1 School of Life Science, Ludong University, Yantai 264025, China;
2 Analysis and Testing Center, Shandong University of Technology, Zibo 255049, China
Abstract:
Little is known about the roles of dynorphin and Kappa opioid receptor (KOR) in mollusks. In this study, we aim to determine the distribution of dynorphin A and KOR-1 in the digestive system of the scallop Chlamys farreri. Using immunohistochemical staining, we confirmed the expression of dynorphin A and KOR-1 in the digestive system of C. farreri. Dynorphin A immunopositive cells were identified in intestine and hepatopancreas. In intestine, small and spherical dynorphin A immunopositive cells (4-9 μm in diameter) were scattered among the long columnar epithelial cells (ECs). In hepatopancreas, cells containing masses (5-14 μm in diameter) of dynorphin A immunopositive products were observed in epithelium of acinis. These immunopositive cells may be synthetic and/or secretory cells of dynorphin A. Dynorphin A immunoreactive products were commonly observed in epithelium and connective tissue (CT) of labial palps, mouth labia and stomach, which presented in forms of grains, fibers or flakes. KOR-1 immunoreactive material was observed in ECs and CTs of labial palps, mouth labia and stomach, intestine and hepatopancreas. The distribution of both dynorphin A and KOR-1 in the digestive organs suggests an involvement of dynorphin via KOR-1 in the functional regulation of the digestive system of C. farreri.
Key words:    Chlamys farreri|dynorphin|digestive system|kappa opioid receptors|immunohistochemistry   
Received: 2017-11-11   Revised:
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Articles by WANGYiyan
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