Cite this paper:
SUN Hushan, WANGYiyan, LIU Xiaoli, LIU Dongwu. Immunohistochemical identification of dynorphin A and Kappa opioid receptor-1 in the digestive system of scallop Chlamys farreri[J]. HaiyangYuHuZhao, 2018, 36(6): 2288-2296

Immunohistochemical identification of dynorphin A and Kappa opioid receptor-1 in the digestive system of scallop Chlamys farreri

SUN Hushan1, WANGYiyan1, LIU Xiaoli1, LIU Dongwu2
1 School of Life Science, Ludong University, Yantai 264025, China;
2 Analysis and Testing Center, Shandong University of Technology, Zibo 255049, China
Little is known about the roles of dynorphin and Kappa opioid receptor (KOR) in mollusks. In this study, we aim to determine the distribution of dynorphin A and KOR-1 in the digestive system of the scallop Chlamys farreri. Using immunohistochemical staining, we confirmed the expression of dynorphin A and KOR-1 in the digestive system of C. farreri. Dynorphin A immunopositive cells were identified in intestine and hepatopancreas. In intestine, small and spherical dynorphin A immunopositive cells (4-9 μm in diameter) were scattered among the long columnar epithelial cells (ECs). In hepatopancreas, cells containing masses (5-14 μm in diameter) of dynorphin A immunopositive products were observed in epithelium of acinis. These immunopositive cells may be synthetic and/or secretory cells of dynorphin A. Dynorphin A immunoreactive products were commonly observed in epithelium and connective tissue (CT) of labial palps, mouth labia and stomach, which presented in forms of grains, fibers or flakes. KOR-1 immunoreactive material was observed in ECs and CTs of labial palps, mouth labia and stomach, intestine and hepatopancreas. The distribution of both dynorphin A and KOR-1 in the digestive organs suggests an involvement of dynorphin via KOR-1 in the functional regulation of the digestive system of C. farreri.
Key words:    Chlamys farreri|dynorphin|digestive system|kappa opioid receptors|immunohistochemistry   
Received: 2017-11-11   Revised:
PDF (2077 KB) Free
Print this page
Add to favorites
Email this article to others
Articles by SUN Hushan
Articles by WANGYiyan
Articles by LIU Xiaoli
Articles by LIU Dongwu
Bohn L M, Belcheva M M, Coscia C J. 2000. Mitogenic signaling via endogenous κ-opioid receptors in C6 glioma cells:evidence for the involvement of protein kinase C and the mitogen-activated protein kinase signaling cascade. Journal of Neurochemistry, 74(2):564-573.
Cahill C M, Taylor A M W, Cook C, Ong E, Morón J A, Evans C J. 2014. Does the kappa opioid receptor system contribute to pain aversion? Frontiers in Pharmacology, 5:253.
Capasso R, Borrelli F, Cascio M G, Aviello G, Huben K, Zjawiony J K, Marini P, Romano B, Di Marzo V, Capasso F, Izzo A A. 2008. Inhibitory effect of salvinorin A, from Salvia divinorum, on ileitis-induced hypermotility:crosstalk between κ-opioid and cannabinoid CB1 receptors.British Journal of Pharmacology, 155(5):681-689.
Cetin Y. 1985. Immunohistochemistry of β-neoendorphin and dynorphin in the endocrine pancreas of rat and man.Histochemistry, 83(4):369-373.
Czyzyk T A, Nogueiras R, Lockwood J F, McKinzie J H, Coskun T, Pintar J E, Hammond C, Tschöp M H, Statnick M A. 2010. kκ-Opioid receptors control the metabolic response to a high-energy diet in mice. FASEB Journal, 24(4):1 151-1 159.
Espinosa E P, Perrigault M, Ward J E, Shumway S E, Allam B. 2009. Lectins associated with the feeding organs of the oyster Crassostrea virginica can mediate particle selection. The Biological Bulletin, 217(2):130-141.
Green I C, Perrin D, Penman E, Yaseen A, Ray K, Howell S L. 1983. Effect of dynorphin on insulin and somatostatin secretion, calcium uptake, and c-AMP levels in isolated rat islets of Langerhans. Diabetes, 32(8):685-690.
Ji B Y, Liu H Q, Zhang R M, Jiang Y L, Wang C M, Li S, Chen J, Bai B. 2017. Novel signaling of dynorphin at κ-opioid receptor/bradykinin B2 receptor heterodimers. Cellular Signalling, 31:66-78.
Kavaliers M, Rangeley R W, Hirst M, Teskey G C. 1986. Muand kappa-opiate agonists modulate ingestive behaviors in the slug, Limax maximus. Pharmacology Biochemistry and Behavior, 24(3):561-566.
Khawaja X Z, Green I C, Thorpe J R, Bailey C J. 1990.
Increased sensitivity to insulin-releasing and glucoregulatory effects of dynorphin A1-13 and U 50488h in ob/ob versus lean mice. Diabetes, 39(10):1 289-1 297.
Le Merrer J, Becker J A, Befort K, Kieffer B L. 2009. Reward processing by the opioid system in the brain. Physiological Reviews, 89(4):1 379-1 412.
Liu D W, Sun H S. 2008. Immunohistological detection of leucine-enkephalin in the digestive system of the scallop Chlamys farreri. International Journal of Peptide Research and Therapeutics, 14(2):137-141.
Liu D W, Sun H S. 2010. Immunohistological detection of mu, delta and kappa opioid-like receptors in the gill, gonad, and hemocytes of the scallop Chlamys farreri. Connective Tissue Research, 51(1):67-70.
Lokensgard J R, Gekker G, Peterson P K. 2002. Kappa-opioid receptor agonist inhibition of HIV-1 envelope glycoprotein-mediated membrane fusion and CXCR4 expression on CD4+ lymphocytes. Biochemical Pharmacology, 63(6):1 037-1 041.
Miller-Perez C, Sanchez-Islas E, Pellicer F, Rodriguez-Manzo G, Cruz S L, Leon-Olea M. 2008. Role of nociceptin/orphanin FQ and the pseudopeptide[Phe1Ψ(CH2NH)Gly2]-nociceptin(1-13)-NH2 and their interaction with classic opioids in the modulation of thermonociception in the land snail Helix aspersa. European Journal of Pharmacology, 581(1-2):77-85.
Morgan M, Heffernan A, Benhabib F, Wagner S, Hewavitharana A K, Shaw P N, Cabot P J. 2017. The efficacy of Dynorphin fragments at the κ, μ and δ opioid receptor in transfected HEK cells and in an animal model of unilateral peripheral inflammation. Peptides, 89:9-16.
Rubin D C, Shaker A, Levin M S. 2012. Chronic intestinal inflammation:inflammatory bowel disease and colitisassociated colon cancer. Frontiers in Immunology, 3:107.
Ruegg H, Yu W Z, Bodnar R J. 1997. Opioid-receptor subtype agonist-induced enhancements of sucrose intake are dependent upon sucrose concentration. Physiology & Behavior, 62(1):121-128.
Sengupta J N. 2009. Visceral pain:the neurophysiological mechanism. Handbook of Experimental Pharmacology, (194):31-74.
Sha A L, Sun H S, Wang Y Y. 2012a. Immunohistochemical study of leucine-enkephalin and delta opioid receptor in mantles and feet of the octopus Octopus ocellatus Gray.International Journal of Peptide Research and Therapeutics, 18(1):71-76.
Sha A L, Sun H S, Wang Y Y. 2012b. Leucine-enkephalin immunoreactivity is also present in the nervous and digestive systems of the octopus, Octopus ocellatus.Marine and Freshwater Behaviour and Physiology, 45(2):155-160.
Sha A L, Sun H S, Wang Y Y. 2013. Immunohistochemical observations of methionine-enkephalin and delta opioid receptor in the digestive system of Octopus ocellatus.Tissue and Cell, 45(1):83-87.
Sobczak M, Sałaga M, Storr M A, Fichna J. 2014. Physiology, signaling, and pharmacology of opioid receptors and their ligands in the gastrointestinal tract:current concepts and future perspectives. Journal of Gastroenterology, 49(1):24-45.
Stefano G B, Salzet-Raveillon B, Salzet M. 1998. Mytilus edulis hemolymph contain prodynorphin. Immunology Letters, 63(1):33-39.
Sun Y, Zhu L, Huang X, Zhou C, Zhang X. 2014.Immunohistochemical localization of nerve fibers in the pseudocapsule of fibroids. European Journal of Histochemistry, 58(2):2 249.
Varaksin A A, Usheva L N, Zverkov I V. 1990. Location of immunoreactive leucine enkephalin in the digestive gland of the Mollusca Mizuhopecten yessoensis (Jay). Arkhiv Anatomii, Gistologii i Embriologii, 98(1):71-74.
Wallace E F, Weber E, Barchas J D, Evans C J. 1984. A putative processing enzyme from Aplysia that cleaves dynorphin A at the single arginine residue. Biochemical and Biophysical Research Communications, 119(1):415-422.
Wang Y Y, Sun S H. 2010. Immunohistochemical identification of met-enkephalin in digestive system and its effect on digestive enzyme activities of the scallop Chlamys farreri.International Journal of Peptide Research and Therapeutics, 16(1):31-37.
Winters B L, Gregoriou G C, Kissiwaa S A, Wells O A, Medagoda D I, Hermes S M, Burford N T, Alt A, Aicher S A, Bagley E E. 2017. Endogenous opioids regulate moment-to-moment neuronal communication and excitability. Nature Communications, 8:14 611.
Wood J D, Galligan J J. 2004. Function of opioids in the enteric nervous system. Neurogastroenterology and Motility, 16(Suppl 2):17-28.
Xiao J, Zeng S, Wang X R, Babazada H, Li Z C, Liu R Y, Yu W F. 2016. Neurokinin 1 and opioid receptors:relationships and interactions in nervous system.Translational Perioperative and Pain Medicine, 1(3):11-21.
Yurdaydin C, Li Y, Ha J H, Jones E A, Rothman R, Basile A S. 1995. Brain and plasma levels of opioid peptides are altered in rats with thioacetamide-induced fulminant hepatic failure:implications for the treatment of hepatic encephalopathy with opioid antagonists. The Journal of Pharmacology and Experimental Therapeutics, 273(1):185-192.