Cite this paper:
SHAO Ting, QIN Chuanjie, DUAN Huiguo, YUAN Dengyue, WEN Zhengyong, WANG Jun, GE Fanglan. Effects of feeding time on complement component C7 expression in Pelteobagrus vachellii subject to bacterial challenge[J]. HaiyangYuHuZhao, 2018, 36(6): 2358-2367

Effects of feeding time on complement component C7 expression in Pelteobagrus vachellii subject to bacterial challenge

SHAO Ting1,2, QIN Chuanjie2, DUAN Huiguo2, YUAN Dengyue2, WEN Zhengyong2, WANG Jun2, GE Fanglan1
1 College of Life Science, Sichuan Normal University, Chengdu 610101, China;
2 College of Life Science, Neijiang Normal University, Key Laboratory of Sichuan Province for Fishes Conservation and Utilization in the Upper Reaches of the Yangtze River, Neijiang 641000, China
Abstract:
Shifting the feeding time for fish from daytime to nighttime could alter their digestive behavior, disturb their metabolism, and may affect immune-related genes. This study aimed to clone complement component C7 and analyze the different expression of C7 mRNA in fish fed during either the day or at night and then challenged with Aeromonas hydrophila infection. The Pv-C7 cDNA of Pelteobagrus vachellii contained 2 647 bp with an open reading frame encoding a protein of 818 amino acids. Multiple sequences analysis indicated that Pv-C7 included eight domains, which was similar to results for other species. Quantitative PCR analysis showed that Pv-C7 was mainly expressed in the liver, spleen, intestine and head kidney tissues of healthy P. vachellii. Quantitative PCR analysis showed that C7 mRNA transcript in the liver, spleen and head kidney also increased significantly when the fish were fed at nighttime (20:00). In addition, the expression of Pv-C7 mRNA significantly increased with A. hydrophila challenge in the liver (48-96 h), spleen and head kidney (12-96 h) tissues of P. vachellii. Pv-C7 mRNA expression of the fish fed at nighttime showed significant higher than that in the fish fed at day time at 12-48 h in head kidney and 12-24 h in spleen. This study indicates that altering the feeding time from daytime to nighttime could increase Pv-C7 mRNA expression, and feeding time may affect the immune response involving C7.
Key words:    Pelteobagrus vachellii|complement component C7|shifting feeding time|clock   
Received: 2017-10-11   Revised:
Tools
PDF (560 KB) Free
Print this page
Add to favorites
Email this article to others
Authors
Articles by SHAO Ting
Articles by QIN Chuanjie
Articles by DUAN Huiguo
Articles by YUAN Dengyue
Articles by WEN Zhengyong
Articles by WANG Jun
Articles by GE Fanglan
References:
Arjona A, Sarkar D K. 2006. Evidence supporting a circadian control of natural killer cell function. Brain, Behavior, and Immunity, 20(5):469-476.
Bossi F, Rizzi L, Bulla R, Debeus A, Tripodo C, Picotti P, Betto E, Macor P, Pucillo C, Würzner R, Tedesco F. 2009. C7 is expressed on endothelial cells as a trap for the assembling terminal complement complex and may exert antiinflammatory function. Blood, 113(15):3 640-3 648.
Bozek K, Relógio A, Kielbasa S M, Heine M, Dame C, Kramer A, Herzel H. 2009. Regulation of clock-controlled genes in mammals. PLoS One, 4(3):e4882.
Castanon-Cervantes O, Wu M W, Ehlen J C, Paul K, Gamble K L, Johnson R L, Besing R C, Menaker M, Gewirtz A T, Davidson A J. 2010. Dysregulation of inflammatory responses by chronic circadian disruption. The Journal of Immunology, 185(10):5 796-5 805.
del Pozo A, Montoya A, Vera L M, Sánchez-Vázquez F J. 2012. Daily rhythms of clock gene expression, glycaemia and digestive physiology in diurnal/nocturnal European seabass. Physiology & Behavior, 106(4):446-450.
Fisheries and Fisheries Administration Bureau of the Ministry of Agriculture. 2017. China Fishery Statistical Yearbook.China Agriculture Press, Beijing. p.25.
Fujito N T, Sugimoto S, Nonaka M. 2010. Evolution of thioester-containing proteins revealed by cloning and characterization of their genes from a cnidarian sea anemone, Haliplanella lineate. Developmental & Comparative Immunology, 34(7):775-784.
Gao B, Radaeva S, Park O. 2009. Liver natural killer and natural killer T cells:immunobiology and emerging roles in liver diseases. Journal of Leukocyte Biology, 86(3):513-528.
González S, Martínez-Borra J, López-Larrea C. 2003. Cloning and characterization of human complement component C7 promoter. Genes and Immunity, 4(1):54-59.
Guo B Y, Wu C W, Lv Z M, Liu C L. 2016. Characterisation and expression analysis of two terminal complement components:C7 and C9 from large yellow croaker, Larimichthys crocea. Fish & Shellfish Immunology, 51:211-219.
Kalsbeek A, Scheer F A, Perreau-Lenz S, La Fleur S E, Yi C X, Fliers E, Buijs R M. 2011. Circadian disruption and SCN control of energy metabolism. FEBS Letters, 585(10):1 412-1 426.
Katagiri T, Hirono I, Aoki T. 1999. Molecular analysis of complement component C8β and C9 cDNAs of Japanese flounder, Paralichthys olivaceus. Immunogenetics, 50(1-2):43-48.
Knutsson A. 2003. Health disorders of shift workers.Occupational Medicine, 53(2):103-108.
Lazado C C, Skov P V, Pedersen P B. 2016. Innate immune defenses exhibit circadian rhythmicity and differential temporal sensitivity to a bacterial endotoxin in Nile tilapia(Oreochromis niloticus). Fish & Shellfish Immunology, 55:613-622.
Li L, Chang M X, Nie P. 2007. Molecular cloning, promoter analysis and induced expression of the complement component C9 gene in the grass carp Ctenopharyngodon idella. Veterinary Immunology and Immunopathology, 118(3-4):270-282.
Li M F. 2010. Progress on study on biology of Pelteeobaagrus fulvidraco (Richardson). Modern Fisheries Information, 25(9):16-22. (in Chinese with English abstract)
Liu J G, Malkani G, Shi X Y, Meyer M, Cunningham-Runddles S, Ma X J, Sun Z S. 2006. The circadian clock Period 2 gene regulates gamma interferon production of NK cells in host response to lipopolysaccharide-induced endotoxic shock. Infection and Immunity, 74(8):4 750-4 756.
Liu W, Jiang L H, Dong X L, Liu X X, Kang L S, Wu C W. 2016. Molecular characterization and expression analysis of the large yellow croaker (Larimichthys crocea)complement component C6 after bacteria challenge.Aquaculture, 458:107-112.
Livak K J, Schmittgen T D. 2001. Analysis of relative gene expression data using Q real-time quantitative PCR and the 2-△△CT method. Methods, 25(4):402-408.
Logan R W, Sarkar D K. 2012. Circadian nature of immune function. Molecular and Cellular Endocrinology, 349(1):82-90.
Montoya A, López-Olmeda J F, Garayzar A B S, SánchezVázquez F J. 2010a. Synchronization of daily rhythms of locomotor activity and plasma glucose, cortisol and thyroid hormones to feeding in Gilthead seabream (Sparus aurata) under a light-dark cycle. Physiology & Behavior, 101(1):101-107.
Montoya A, López-Olmeda J F, Yúfera M, Sánchez-Muros M J, Sánchez-Vázquez F J. 2010b. Feeding time synchronises daily rhythms of behaviour and digestive physiology in gilthead seabream (Sparus aurata). Aquaculture, 306(1-4):315-321.
Morgan B P, Marchbank K J, Longhi M P, Harris C L, Gallimore A M. 2005. Complement:central to innate immunity and bridging to adaptive responses. Immunology Letters, 97(2):171-179.
Mukherji A, Kobiitaa A, Chambona P. 2015. Shifting the feeding of mice to the rest phase creates metabolic alterations, which, on their own, shift the peripheral circadian clocks by 12 hours. Proceedings of the National Academy of Sciences of the Unite States of America, 112(48):E6 683-E6 690.
Muller-Eberhard H J. 1986. The membrane attack complex of complement. Annual Review of Immunology, 4(1):503-528.
Niroshana Wickramaarachchi W D, Whang I, Kim E, Lim B S, Jeong H-B, De Zoysa M, Oh M-J, Jung S-J, Yeo S-Y, Yeon K S, Park H-C, Lee J. 2013. Genomic characterization and transcriptional evidence for the involvement of complement component 7 in immune response of rock bream (Oplegnathus fasciatus). Developmental & Comparative Immunology, 41(1):44-49.
Oishi K, Ohkura N, Kadota K, Kasamatsu M, Shibusawa K, Matsuda J, Machida K, Horie S, Ishida N. 2006. Clock mutation affects circadian regulation of circulating blood cells. Journal of Circadian Rhythms, 4:13.
Phillips D J, Savenkova M I, Karatsoreos I N. 2015.Environmental disruption of the circadian clock leads to altered sleep and immune responses in mouse. Brain, Behavior, and Immunity, 47:14-23.
Qin C J, Gong Q, Wen Z Y, Yuan D Y, Shao T, Wang J, Li H T. 2017. Transcriptome analysis of the spleen of the darkbarbel catfish Pelteobagrus vachellii in response to Aeromonas hydrophila infection. Fish & Shellfish Immunology, 70:498-506.
Qin C J, Shao T. 2015. The Clock gene clone and its circadian rhythms in Pelteobagrus vachelli. Chinese Journal of Oceanology and Limnology, 33(3):597-603.
Scheidereit C. 2006. IκB kinase complexes:gateways to NF-κB activation and transcription. Oncogene, 25(51):6 685-6 705.
Shen Y B, Zhang J B, Xu X Y, Fu J J, Li J L. 2012. Expression of complement component C7 and involvement in innate immune responses to bacteria in grass carp. Fish & Shellfish Immunology, 33(2):448-454.
Sun Y Y, Wang R X, Xu T J. 2013. Conserved structural complement component C3 in miiuy croaker Miichthys miiuy and their involvement in pathogenic bacteria induced immunity. Fish & Shellfish Immunology, 35(1):184-187.
Wang S C, Gao Y H, Shu C, Xu T J. 2015. Characterization and evolutionary analysis of duplicated C7 in miiuy croaker.Fish & Shellfish Immunology, 45(2):672-679.
Wang S C, Wang R X, Xu T J. 2013. The evolutionary analysis on complement genes reveals that fishes C3 and C9 experience different evolutionary patterns. Fish & Shellfish Immunology, 35(6):2 040-2 045.
Witzel-Schlomp K, Rittner C, Schneider P M. 2001. The human complement C9 gene:structural analysis of the 5'gene region and genetic polymorphism studies. European Journal of Immunogenetics, 28(5):515-522.
Yang R B, Xie C X, Wei K J, Zheng W Y, Lei C S, Feng K. 2006. The daily feeding rhythms of juvenile yellow catfish, Pelteobagrus fulvidraco at different feeding frequencies. Journal of Huazhong Agricultural University, 25(3):274-276. (in Chinese with English abstract)
Zheng K K, Zhu X M, Han D, Yang Y X, Lei W, Xie S Q. 2010.Effects of dietary lipid levels on growth, survival and lipid metabolism during early ontogeny of Pelteobagrus vachelli larvae. Aquaculture, 299(1-4):121-127.