Cite this paper:
QIN Chuanjie, SHAO Ting. The Clock gene clone and its circadian rhythms in Pelteobagrus vachelli[J]. Journal of Oceanology and Limnology, 2015, 33(3): 597-603

The Clock gene clone and its circadian rhythms in Pelteobagrus vachelli

QIN Chuanjie, SHAO Ting
College of Life Sciences, Neijiang Normal University, Neijiang 641000, China
Abstract:
The Clock gene, a key molecule in circadian systems, is widely distributed in the animal kingdom. We isolated a 936-bp partial cDNA sequence of the Clock gene (Pva-clock) from the darkbarbel catfish Pelteobagrus vachelli that exhibited high identity with Clock genes of other species of fish and animals (65%-88%). The putative domains included a basic helix-loop-helix (bHLH) domain and two period-ARNT-single-minded (PAS) domains, which were also similar to those in other species of fish and animals. Pva-Clock was primarily expressed in the brain, and was detected in all of the peripheral tissues sampled. Additionally, the pattern of Pva-Clock expression over a 24-h period exhibited a circadian rhythm in the brain, liver and intestine, with the acrophase at zeitgeber time 21:35, 23:00, and 23:23, respectively. Our results provide insight into the function of the molecular Clock of P. vachelli.
Key words:    Clock gene|Pelteobagrus vachelli|circadian rhythms|circadian gene   
Received: 2014-07-20   Revised: 2014-10-28
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Articles by QIN Chuanjie
Articles by SHAO Ting
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