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ZHOU Liqing, WANG Xuemei, WU Biao, SUN Xiujun, ZHAO Qing, ZHANG Gaowei, LIU Zhihong, YANG Aiguo. Karyological analysis of the sea cicada Blepharipoda liberate Shen from the Rizhao intertidal zone, China[J]. HaiyangYuHuZhao, 2019, 37(1): 169-175

Karyological analysis of the sea cicada Blepharipoda liberate Shen from the Rizhao intertidal zone, China

ZHOU Liqing1,3, WANG Xuemei2, WU Biao1,3, SUN Xiujun1,3, ZHAO Qing1, ZHANG Gaowei1, LIU Zhihong1,3, YANG Aiguo1,3
1 Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;
2 Rizhao Ocean & Fisheries Research Institute, Rizhao 276826, China;
3 Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China
Blepharipoda liberate Shen is a commercially valuable seafood species that has important ecological significance in Shandong Province, China. Although B. liberate is crustacean, its external characteristics are not entirely those of shrimps or crabs. The question of whether B. liberate is a shrimp or a crab has been debated in recent years. We studied the karyotype of B. liberate by light microscopy using air-drying and spreading methods. We obtained mitotic chromosomal plates from B. liberate larvae, and from adult B. liberate females subsequent to egg-laying. The results revealed that B. liberate has 53 pairs of chromosomes (i.e., n=53 and 2n=106), a characteristic shared with four species of crab. The karyogram of B. liberate consists of 25 metacentric, 14 submetacentric, 11 subtelocentric and 3 telocentric pairs. We did not find any heteromorphosis sex chromosomes. Tissue from larvae, gills and ovaries can be used for chromosomal investigations, and we found similar lampbrush chromosomes in ovary cells. Comparatively speaking, larvae tissue is more practical, and ovary tissue is more suitable for the preparation of lampbrush chromosomes. B. liberate is more closely related to crabs than to shrimps, based on the numbers of chromosomes. The B. liberate karyotype reported here provides a basis for further comparative cytogenetic studies of species populations.
Key words:    Blepharipoda liberate|chromosome|karyotype|lampbrush chromosome   
Received: 2017-11-27   Revised: 2018-02-05
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