Cite this paper:
YONG Wai-Kuan, LIM Phaik-Eem, VELLO Vejeysri, SIM Kae-Shin, ABDUL MAJID Nazia, MUSTAFA Emienour Muzalina, NIK SULAIMAN Nik Meriam, LIEW Kan-Ern, CHEN Brenna Jia-Tian, PHANG Siew-Moi. Metabolic and physiological regulation of Chlorella sp. (Trebouxiophyceae, Chlorophyta) under nitrogen deprivation[J]. HaiyangYuHuZhao, 2019, 37(1): 186-198

Metabolic and physiological regulation of Chlorella sp. (Trebouxiophyceae, Chlorophyta) under nitrogen deprivation

YONG Wai-Kuan1,2, LIM Phaik-Eem1, VELLO Vejeysri1,3, SIM Kae-Shin3, ABDUL MAJID Nazia3, MUSTAFA Emienour Muzalina4, NIK SULAIMAN Nik Meriam5, LIEW Kan-Ern6, CHEN Brenna Jia-Tian7, PHANG Siew-Moi1,3
1 Institute of Ocean and Earth Sciences, University of Malaya, 50603 Kuala Lumpur, Malaysia;
2 Institute of Graduate Studies, University of Malaya, 50603 Kuala Lumpur, Malaysia;
3 Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia;
4 School of Fisheries and Aquaculture Sciences, University of Malaysia, Terengganu, T145, 21300 Kuala Terengganu, Terengganu, Malaysia;
5 Department of Chemical Engineering, Faculty of Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia;
6 Airbus Group Malaysia, Menara HLA, 3, Jalan Kia Peng, 50450 Kuala Lumpur, Malaysia;
7 Aerospace Malaysia Innovation Centre, Level 1, MIGHT Building, 3517, Jalan Teknokrat 5, 63000 Cyberjaya, Selangor, Malaysia
Abstract:
A freshwater green microalgae Chlorella sp., UMACC344 was shown to produce high lipid content and has the potential to be used as feedstock for biofuel production. In this study, photosynthetic efficiency, biochemical profiles and non-targeted metabolic profiling were studied to compare between the nitrogen-replete and deplete conditions. Slowed growth, change in photosynthetic pigments and lowered photosynthetic efficiency were observed in response to nitrogen deprivation. Biochemical profiles of the cultures showed an increased level of carbohydrate, lipids and total fatty acids, while the total soluble protein content was lowered. A trend of fatty acid saturation was observed in the nitrogen-deplete culture with an increase in the level of saturated fatty acids especially C16:0 and C18:0, accompanied by a decrease in proportions of monounsaturated and polyunsaturated fatty acids. Fifty-nine metabolites, including amino acids, lipids, phytochemical compounds, vitamins and cofactors were significantly dysregulated and annotated in this study. Pathway mapping analysis revealed a rewiring of metabolic pathways in the cells, particularly purine, carotenoid, nicotinate and nicotinamide, and amino acid metabolisms. Within the treatment period of nitrogen deprivation, the key processes involved were reshuffling of nitrogen from proteins and photosynthetic machinery, together with carbon repartitioning in carbohydrates and lipids.
Key words:    metabolic profiling|Chlorella sp.|nitrogen stress|lipid|fatty acid   
Received: 2017-09-11   Revised: 2017-11-20
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Articles by YONG Wai-Kuan
Articles by LIM Phaik-Eem
Articles by VELLO Vejeysri
Articles by SIM Kae-Shin
Articles by ABDUL MAJID Nazia
Articles by MUSTAFA Emienour Muzalina
Articles by NIK SULAIMAN Nik Meriam
Articles by LIEW Kan-Ern
Articles by CHEN Brenna Jia-Tian
Articles by PHANG Siew-Moi
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