Cite this paper:
SHI Ce, ZENG Tinglan, LI Ronghua, WANG Chunlin, YE Yangfang, MU Changkao. Dynamic metabolite alterations of Portunus trituberculatus during larval development[J]. HaiyangYuHuZhao, 2019, 37(1): 361-373

Dynamic metabolite alterations of Portunus trituberculatus during larval development

SHI Ce1, ZENG Tinglan1, LI Ronghua1, WANG Chunlin1, YE Yangfang1,2, MU Changkao1
1 Key Laboratory of Applied Marine Biotechnology, Ningbo University, Chinese Ministry of Education, Ningbo 315211, China;
2 Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, Ningbo 315211, China
Abstract:
A mass mortality often occurs from molting to the megalopa stage during the larval development of the swimming crab Portunus trituberculatus. Larvae with insufficient nutrient accumulation during the zoeal stages are probably unable to develop into juvenile swimming crabs. However, the nutritional information such as the primary metabolites is scarce for P. trituberculatus larvae. The aim of this work is to obtain an insight into the metabolite traits of P. trituberculatus at early developmental stages. 1H nuclear magnetic resonance spectroscopy coupled with multivariate data analysis was used to determine how the metabolite profiles shift during larval development in P. trituberculatus. Our results show that the trend of total metabolites exhibited a rise from zoea 1 to zoea 3, followed by a drop from zoea 4 to megalopa and recovery during the first juvenile stage. A large-scale depletion of total metabolites in the zoea 4 and megalopa stages suggests a deep depression of metabolic activity, which may be linked to the mass mortality from molting to the megalopa stage. These findings provided essential metabolic information about the larval development of P. trituberculatus and important clues for understanding the nutritional requirements of P. trituberculatus in early developmental stages.
Key words:    Portunus trituberculatus|larval development|metabolite phenotype|nuclear magnetic resonance (NMR)   
Received: 2017-10-08   Revised: 2017-12-13
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References:
Andrés M, Estévez A, Hontoria F, Rotllant G. 2010. Differential utilization of biochemical components during larval development of the spider crab Maja brachydactyla(Decapoda:Majidae). Marine Biology, 157(10):2 329-2 340.
Anger K. 1998. Patterns of growth and chemical composition in decapod crustacean larvae. Invertebrate Reproduction & Development, 33(2-3):159-176.
Anger K. 2001. The Biology of Decapod Crustacean Larvae.A.A. Balkem, Lisse.
Aue W P, Bartholdi E, Ernst R R. 1976a. Two-dimensional spectroscopy. Application to nuclear magnetic resonance.The Journal of Chemical Physics, 64(5):2 229-2 246.
Aue W P, Karhan J, Ernst R R. 1976b. Homonuclear broad band decoupling and two-dimensional J-resolved NMRspectroscopy. The Journal of Chemical Physics, 64(10):4 226-4 227.
Augusto A, Greene L J, Laure H J, McNamara J C. 2007.Adaptive shifts in osmoregulatory strategy and the invasion of freshwater by brachyuran crabs:evidence from Dilocarcinus pagei (trichodactylidae). Journal of Experimental Zoology, 307A(12):688-698.
Avella M, Ducoudret O, Pisani D F, Poujeol P. 2009. Swellingactivated transport of taurine in cultured gill cells of sea bass:physiological adaptation and pavement cell plasticity. American Journal of Physiology Regulatory, Integrative and Comparative Physiology, 296(4):R1 149-R1 160.
Boisen S. 2003. Ideal dietary amino acid profiles for pigs. In:D'Mello J P F ed. Amino Acids in Animal Nutrition.CABI, Edinburgh, United Kingdom. p.103-123.
Braunschweiler L, Ernst R R. 1983. Coherence transfer by isotropic mixing:application to proton correlation spectroscopy. Journal of Magnetic Resonance (1969), 53(3):521-528.
Brucet S, Boix D, López-Flores R, Badosa A, Quintana X D. 2005. Ontogenic changes of amino acid composition in planktonic crustacean species. Marine Biology, 148(1):131-139.
Chen S L, Wu X G, Cheng Y X, Wang C L, Zhu D F, Zhou B, Wang J F, Gong L J. 2007. Changes of proximate biochemical composition and energy source during embryonic development of swimming crab, Portunus trituberculatus. Journal of Fishery Sciences of China, 14(2):229-235. (in Chinese with English abstract)
Cloarec O, Dumas M E, Trygg J, Craig A, Barton R H, Lindon J C, Nicholson J K, Holmes E. 2005. Evaluation of the orthogonal projection on latent structure model limitations caused by chemical shift variability and improved visualization of biomarker changes in 1H NMR spectroscopic metabonomic studies. Analytical Chemistry, 77(2):517-526.
Committee on the Nutrient Requirements of Fish and Shrimp, Board on Agriculture and Natural Resources, National Research Council. 2011. Nutrient Requirements of Fish and Shrimp. National Academies Press, Washington, DC.p.57-92.
Conlan J A, Jones P L, Turchini G M, Hall M R, Francis D S. 2014. Changes in the nutritional composition of captive early-mid stage Panulirus ornatus phyllosoma over ecdysis and larval development. Aquaculture, 434:159-170.
Dan S, Kaneko T, Takeshima S, Ashidate M, Hamasaki K. 2013. Variations in larval morphology and their relationships to survival during mass seed production by the swimming crab, Portunus trituberculatus (Brachyura, Portunidae). Aquaculture, 414-415:109-118.
Eriksson L, Trygg J, Wold S. 2008. CV-ANOVA for significance testing of PLS and OPLS® models. Journal Chemometrics, 22(11-12):594-600.
Fan T W M. 1996. Metabolite profiling by one- and twodimensional NMR analysis of complex mixtures. Progress in Nuclear Magnetic Resonance Spectroscopy, 28(2):161-219.
Hammer K M, Pedersen S A, Størseth T R. 2012. Elevated seawater levels of CO2 change the metabolic fingerprint of tissues and hemolymph from the green shore crab Carcinus maenas. Comparative Biochemistry and Physiology Part D:Genomics and Proteomics, 7(3):292-302.
Hao G J, Lin F, Mu C K, Li R H, Yao J Y, Yuan X M, Pan X Y, Shen J Y, Wang C L. 2015. SNP E4-205 C/T in C-type lectin of Portunus trituberculatus is association with susceptibility/resistance to Vibrio alginolyticus challenge.Aquaculture, 442:125-131.
Haond C, Bonnal L, Sandeaux R, Charmantier G, Trilles J P. 1999. Ontogeny of intracellular isosmotic regulation in the European lobster Homarus gammarus (L.).Physiological and Biochemical Zoology, 72(5):534-544.
Hird F J R. 1986. The importance of arginine in evolution.Comparative Biochemistry and Physiology Part B:Comparative Biochemistry, 85(2):285-288.
Holme M H, Zeng C S, Southgate P C. 2009. A review of recent progress toward development of a formulated microbound diet for mud crab, Scylla serrata, larvae and their nutritional requirements. Aquaculture, 286(3-4):164-175.
Hu S, Wang J, Han T, Li X, Jiang Y, Wang C. 2016. Effects of dietary DHA/EPA ratios on growth performance, survival and fatty acid composition of juvenile swimming crab(Portunus trituberculatus). Aquaculture Research, 48(3):1 291-1 301.
Ikeda T, Smith G, McKinnon A D, Hall M. 2011. Metabolism and chemical composition of phyllosoma larvae, with special reference to the tropical rock lobster Panulirus ornatus (Decapoda; Palinuridae). Journal of Experimental Marine Biology and Ecology, 405(1-2):80-86.
Jin M, Wang M Q, Huo Y W, Huang W W, Mai K S, Zhou Q C. 2015. Dietary lysine requirement of juvenile swimming crab, Portunus trituberculatus. Aquaculture, 448:1-7.
Jin M, Zhou Q C, Wang M Q, Huo Y W, Huang W W, Mai K S. 2016. Dietary arginine requirement of juvenile swimming crab, Portunus trituberculatus. Aquaculture Nutrition, 22(6):1 174-1 184.
Kean J C, Castell J D, Boghen A G, D'Abramo L R, Conklin D E. 1985. A re-evaluation of the lecitihin and cholesterol requirements of juvenile lobster (Homarus americanus)using crab protein-based diets. Aquaculture, 47(2-3):143-149.
Lim B K, Hirayama K. 1991. Growth and elemental composition (C,N,P) during larval developmental stages of mass-cultured swimming crab Portunus trituberculatus.Marine Ecology Progress Series, 78:131-137.
Mazzarelli C C M, Santos M R, Amorim R V, Augusto A. 2015. Effect of salinity on the metabolism and osmoregulation of selected ontogenetic stages of an amazon population of Macrobrachium amazonicum shrimp (Decapoda, Palaemonidae). Brazilian Journal of Biology, 75(2):372-379.
Millamena O M, Quinitio E. 2000. The effects of diets on reproductive performance of eyestalk ablated and intact mud crab Scylla serrata. Aquaculture, 181(1-2):81-90.
Minagawa M, Chiu J R, Murano M. 1993. Developmental changes in body weight and elemental composition of laboratory-reared larvae of the red frog crab, Ranina ranina (Decapoda:Brachyura). Marine Biology, 116(3):399-406.
Morioka Y, Kitajima C, Hayashida G. 1988. Oxygen consumption, growth and calculated food requirement of the swimming crab Protunus trituberculatus in its early developmental stage. Nippon Suisan Gakkaishi, 54(7):1 137-1 141.
Peñaflorida V D. 2004. Amino acid profiles in the midgut, ovary, developing eggs and zoea of the mud crab, Scylla serrata.Israeli Journal of Aquaculture-Bamidgeh, 56(2):111-123.
Ponat A, Adelung D. 1983. Studies to establish an optimal diet for Carcinus maenas:3. Vitamin and quantitative lipid requirements. Marine Biology, 74(3):275-279.
Preston R L. 1993. Transport of amino acids by marine invertebrates. Journal of Experimental Zoology, 265(4):410-421.
Sheen S S, Wu S W. 1999. The effects of dietary lipid levels on the growth response of juvenile mud crab Scylla serrata.Aquaculture, 175(1-2):143-153.
Sheen S S. 2000. Dietary cholesterol requirement of juvenile mud crab Scylla serrata. Aquaculture, 189(3-4):277-285.
Sun Y M, Yan Y, Sun J J. 1984. The larval development of Portunus trituberculatus. Journal of Fisheries of China, 8(3):219-226. (in Chinese with English abstract)
Suprayudi M A, Takeuchi T, Hamasaki K. 2004. Essential fatty acids for larval mud crab Scylla serrata:implications of lack of the ability to bioconvert C18 unsaturated fatty acids to highly unsaturated fatty acids. Aquaculture, 231(1-4):403-416.
Trygg J, Holmes E, Lundstedt T. 2007. Chemometrics in metabonomics. Journal of Proteome Research, 6(2):469-479.
Trygg J, Wold S. 2002. Orthogonal projections to latent structures (O-PLS). Journal of Chemometrics, 16(3):119-128.
Upmeier B, Gross W, Köster S, Barz W. 1988. Purification and properties of S-adenosyl-L-methionine:Nicotinic acid-Nmethyltransferase from cell suspension cultures of Glycine max L. Archives of Biochemistry and Biophysics, 262(2):445-454.
Van Den Berg R A, Hoefsloot H C J, Westerhuis J A, Smilde A K, Van Der Werf M J. 2006. Centering, scaling, and transformations:improving the biological information content of metabolomics data. BMC Genomics, 7:142.
Wu X G, Zeng C S, Southgate P C. 2014. Ontogenetic patterns of growth and lipid composition changes of blue swimmer crab larvae:insights into larval biology and lipid nutrition.Marine and Freshwater Research, 65(3):228-243.
Xiao C N, Hao F H, Qin X R, Wang Y L, Tang H R. 2009. An optimized buffer system for NMR-based urinary metabonomics with effective pH control, chemical shift consistency and dilution minimization. Analyst, 134(5):916-925.
Xie Z M, Liu H J, Feng L. 2002. Breeding of swimming crab, Portunus trituberculatus. In:Xie Z M ed. Hatchery Technology of Marine Economic Crabs. China Agriculture Press, Beijing, China. p.1-304. (in Chinese)
Yancey P H, Blake W R, Conley J. 2002. Unusual organic osmolytes in deep-sea animals:adaptations to hydrostatic pressure and other perturbants. Comparative Biochemistry and Physiology Part A:Molecular & Integrative Physiology, 133(3):667-676.
Yancey P H. 2005. Organic osmolytes as compatible, metabolic and counteracting cytoprotectants in high osmolarity and other stresses. Journal of Experimental Biology, 208(15):2 819-2 830.
Ye Y F, An Y P, Li R H, Mu C K, Wang C L. 2014. Strategy of metabolic phenotype modulation in Portunus trituberculatus exposed to low salinity. Journal of Agricultural and Food Chemistry, 62(15):3 496-3 503.