Spatial heterogeneity in a deep artificial lake plankton community revealed by PCR-DGGE fingerprinting -- Journal of Oceanology and Limnology,2015,33(3):624-635

	Cite this paper:
	LI Qiang, ZHAO Yue, ZHANG Xu, WEI Yuquan, QIU Linlin, WEI Zimin, LI Fuheng. Spatial heterogeneity in a deep artificial lake plankton community revealed by PCR-DGGE fingerprinting[J]. Journal of Oceanology and Limnology, 2015, 33(3): 624-635

Spatial heterogeneity in a deep artificial lake plankton community revealed by PCR-DGGE fingerprinting

LI Qiang, ZHAO Yue, ZHANG Xu, WEI Yuquan, QIU Linlin, WEI Zimin, LI Fuheng

College of Life Science, Northeast Agricultural University, Harbin 150030, China

Abstract:

To explore the spatial heterogeneity of plankton communities in a deep artificial lake (Songhua Lake, China), samples were collected at seven sites. Samples were investigated by denaturing gradient gel electrophoresis (DGGE) analysis of the PCR-amplified 16S and 18S rRNA genes and specific bands were sequenced. Cluster analysis of the DGGE profiles revealed that all of the samples grouped into two distinct clusters, in accordance with sampling site; while in each cluster, the divergence of sub-clusters correlated with sampling depth. Sequence analysis of selected dominant DGGE bands revealed that most sequenced phylotypes (84%) exhibited ≥97% similarity to the closest sequences in GenBank, and were affiliated with ten common freshwater plankton phyla (Proteobacteria, Actinobacteria, Bacteroidetes, Cyanobacteria, Bacillariophyta, Pyrrophyta, Cryptophyta, Ciliophora, Stramenopiles, and Rotifera). Several of these groups are also found worldwide, indicating the cosmopolitan distribution of the phylotypes. The relationships between DGGE patterns and environmental factors were analyzed by redundancy analysis (RDA). The results suggested that, total nitrogen, nitrate, nitrite, ammonia, and COD_Mn concentrations, and water temperature were strongly correlated with the variation in plankton composition.

Key words: Songhua Lake|plankton community composition|denaturing gradient gel electrophoresis (DGGE)|artificial lake|redundancy analysis

Received: 2014-07-19 Revised: 2014-11-05

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Articles by ZHAO Yue

Articles by ZHANG Xu

Articles by WEI Yuquan

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Articles by WEI Zimin

Articles by LI Fuheng

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