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GU Ying, LU Cuiyun, ZHANG Xiaofeng, LI Chao, YU Juhua, SUN Xiaowen. Genetic mapping and QTL analysis for body weight in Jian carp (Cyprinus carpio var. Jian) compared with mirror carp (Cyprinus carpio L.)[J]. HaiyangYuHuZhao, 2015, 33(3): 636-649

Genetic mapping and QTL analysis for body weight in Jian carp (Cyprinus carpio var. Jian) compared with mirror carp (Cyprinus carpio L.)

GU Ying1, LU Cuiyun1,2, ZHANG Xiaofeng1, LI Chao1, YU Juhua3, SUN Xiaowen1
1 Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China;
2 College of Fisheries and Life Sciences, Shanghai Ocean University, Shanghai 201306, China;
3 Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China
We report the genetic linkage map of Jian carp (Cyprinus carpio var. Jian). An F 1 population comprising 94 Jian carp individuals was mapped using 254 microsatellite markers. The genetic map spanned 1 381.592 cM and comprised 44 linkage groups, with an average marker distance of 6.58 cM. We identified eight quantitative trait loci (QTLs) for body weight (BW) in seven linkage groups, explaining 12.6% to 17.3% of the phenotypic variance. Comparative mapping was performed between Jian carp and mirror carp (Cyprinus carpio L.), which both have 50 chromosomes. One hundred and ninety-eight Jian carp marker loci were found in common with the mirror carp map, with 186 (93.94%) showing synteny. All 44 Jian carp linkage groups could be one-to-one aligned to the 44 mirror carp linkage groups, mostly sharing two or more common loci. Three QTLs for BW in Jian carp were conserved in mirror carp. QTL comparison suggested that the QTL confidence interval in mirror carp was more precise than the homologous interval in Jian carp, which was contained within the QTL interval in Jian carp. The syntenic relationship and consensus QTLs between the two varieties provide a foundation for genomic research and genetic breeding in common carp.
Key words:    Jian carp|mirror carp|body weight|linkage map|QTL|comparative mapping   
Received: 2014-08-18   Revised: 2014-10-21
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