Cite this paper:
LU Zhen, SHAN Xiujuan, JI Chenglong, ZHAO Jianmin, WU Huifeng. Proteomic responses induced by metal pollutions in oysters Crassostrea sikamea[J]. HaiyangYuHuZhao, 2019, 37(2): 685-693

Proteomic responses induced by metal pollutions in oysters Crassostrea sikamea

LU Zhen1,3, SHAN Xiujuan2,4, JI Chenglong1,2, ZHAO Jianmin1, WU Huifeng1,2
1 Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research(YIC), Chinese Academy of Sciences(CAS);Shandong Provincial Key Laboratory of Coastal Zone Environmental Processes, YICCAS, Yantai 264003, China;
2 Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China;
3 University of Chinese Academy of Sciences, Beijing 100049, China;
4 Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture;Shandong Provincial Key Laboratory of Fishery Resources and Ecological Environment, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China
There exist severe metal pollutions along the Jiulongjiang estuary in South China. In order to unravel the biological effects caused by metal pollutions, proteomic responses were investigated by two-dimensional electrophoresis-based proteomics in oysters Crassostrea sikamea from metal pollution sites, Jinshan (JS) and Baijiao (BJ), and a relatively clean site, Jiuzhen (JZ), along the Jiulongjiang estuary. Results indicated that metal pollutions mainly induced cellular injuries, oxidative and immune stresses, and disturbed ion homeostasis in oysters C. sikamea from both JS and BJ sites via differential pathways. Furthermore, metal pollution enhanced transcriptional initiation in oysters from JS site. In addition, the Cu and Fe pollution might be indicated by the 78 kDa glucose regulated protein and ferritin GF1 in oysters C. sikamea, respectively. The study confirms that proteomics is a promising approach to characterize the underlying mechanisms of responses to metal pollution in oysters.
Key words:    metal pollution|Crassostrea sikamea|biological effect|proteomics   
Received: 2018-03-20   Revised: 2018-05-02
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