Cite this paper:
YAO Jianting, SHUAI Li, LI Shengyao, XU Caolu, WANG Xiuliang. Genetic analysis of selected Sargassum fusiforme (Harvey) Setchell (Sargassaceae, Phaeophyta) strains with RAPD and ISSR markers[J]. HaiyangYuHuZhao, 2019, 37(3): 783-789

Genetic analysis of selected Sargassum fusiforme (Harvey) Setchell (Sargassaceae, Phaeophyta) strains with RAPD and ISSR markers

YAO Jianting1,2,3, SHUAI Li4, LI Shengyao5, XU Caolu5, WANG Xiuliang1,2,3
1 Key Lab of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;
2 Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China;
3 Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China;
4 School of Environmental Science and Engineering, Qingdao University, Qingdao 266071, China;
5 Dongtou Yangqicai Research Institute, Wenzhou 325700, China
Abstract:
Since the 1980s, Sargassum fusiforme has been cultivated in Zhejiang, South China, and nowadays it becomes one of the important commercial seaweeds in China. With traditions of eating habits in the East Asian countries, this brown alga is used as food, because it contained functional oligo/polysaccharides and chemical components, and was regarded playing roles in antioxidant activities and regulating immunology. Through over 15 years' selection, breeding and cultivation, we obtained three strains with good traits and testified their characters during the production, which included the cultivars with high yield and other two good characters, either all the selected strains were applied in the Sargassum production. To avoid confusion during the selection and nursery, it was preferred to establish one fingerprint for distinguishing the Sargassum cultivars from different strains. Random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) methods were adopted to analyze the genetic diversities of the selected S. fusiforme strains. With that, one fingerprint with RAPD markers was constructed, and one sequence characterized amplified region (SCAR) marker to S. fusiforme was obtained. It is indicated that the applied fingerprint could be valid in S. fusiforme genetic and germplasm justification, and will be positive to molecular marker assistance in its selection and cultivation.
Key words:    Sargassum fusiforme|random amplified polymorphic DNA (RAPD)|inter-simple sequence repeat (ISSR)|sequence characterized amplified region (SCAR)|genetic analysis   
Received: 2018-05-05   Revised: 2018-08-02
Tools
PDF (774 KB) Free
Print this page
Add to favorites
Email this article to others
Authors
Articles by YAO Jianting
Articles by SHUAI Li
Articles by LI Shengyao
Articles by XU Caolu
Articles by WANG Xiuliang
References:
Choi E Y, Hwang H J, Kim I H, Nam T J. 2009. Protective effects of a polysaccharide from Hizikia fusiformis against ethanol toxicity in rats. Food and Chemical Toxicology, 47(1):134-139, https://doi.org/10.1016/j.fct.2008.10.026.
Choi E Y, Hwang H J, Nam T J. 2010.Protective effect of a polysaccharide from Hizikia fusiformis against ethanolinduced cytotoxicity in IEC-6 cells. Toxicology in Vitro, 24(1):79-84, https://doi.org/10.1016/j.tiv.2009.08.031.
Haraguchi H, Sekida S. 2008. Recent changes in the distribution of Sargassum species in Kochi, Japan. Kuroshio Science, 2:41-46.
Hu Z M, Zhang J, Lopez-Bautista J, Duan D L. 2013.Asymmetric genetic exchange in the brown seaweed Sargassum fusiforme (Phaeophyceae) driven by oceanic currents. Marine Biology, 160(6):1 407-1 414, https://doi.org/10.1007/s00227-013-2192-x.
Ji Y B, Gao S Y, Zhang X J. 2004. Studies on anti-tumor activities of Hizikia fusiformis polysaccharide (SFPS) and its mechanism. Chinese Journal of Marine Drugs, 4:7-10.(in Chinese with English abstract)
Karawita R, Siriwardhana N, Lee K W, Heo M S, Yeo I K, Lee Y D, Jeon Y J. 2004. Reactive oxygen species scavenging, metal chelation, reducing power and lipid peroxidation inhibition properties of different solvent fractions from Hizikia fusiformis. European Food Research and Technology, 220(3-4):363-371, https://doi.org/10.1007/s00217-004-1044-9.
Li S Y, Xu C L, Li J P, Yao J T, Liu J D, Duan D L. 2010.Selection, artificial breeding and cultivation of Hizikia "Lufeng No.1". Progress in Fishery Sciences, 31(2):88-94. (in Chinese with English abstract)
Miller M P. 1997. Tools for population genetic analyses(TFPGA) 1.3:a Windows program for the analysis of allozyme and molecular population genetic data.Computer software distributed by author.
Nan C R, Zhang P, Lin S Z, Wu C Y, Zhang L N. 2015. ISSR analysis of genetic variation to Sargassum fusiformis wild and breeding strains in Wenzhou. Acta Agriculturae Zhejiangensis, 27(2):234-239. (in Chinese with English abstract)
Nei M. 1978. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics, 89:583-590.
Paran I, Michelmore R W. 1993. Development of reliable PCR-based markers linked to downy mildew resistance genes in lettuce. Theoretical and Applied Genetics, 85(8):985-993, https://doi.org/10.1007/BF00215038.
Park J W, Cho Y C, Nam B H, Jin H J, Sohn C H, Hong Y K. 1998. RAPD identification of genetic variation in seaweed Hizikia fusiformis (Fucales, Phaeophyta). Journal of Marine Biotechnology, 6(1):62-64.
Shan T F, Li S Y, Sun J Z, Pang S J. 2009. Morphological comparison of different cultivated strains and AFLP analyses of a typical cultivated population of Hizikia fusiformis at Dongtou, Zhejiang. Journal of Fishery Sciences of China, 16(1):61-68. (in Chinese with English abstract)
Wang W D. 2003. Investigation and study on Sargassum and Hizikia in Zhejiang Province. Journal of Shanghai Fisheries University, 12(3):227-232. (in Chinese with English abstract)
Xu J L, Luo Q J, Yan X J. 2014. Analysis of cultivated characteristics and ISSR of different strains of Sargassum fusiforme (Harv.) Setch. in Dongtou, Zhejang. Journal of Marine Sciences, 32(2):74-79. (in Chinese with English abstract)
Yeh F C, Yang R C, Boyle T, Ye Z H, Mao J X. 1997. Popgene, the user friendly shareware for population genetic analysis. Edmonton, Canada:University of Alberta.
Yu S H, Deng Y Y, Yao J T, Li S Y, Xin X, Duan D L. 2012.Population genetics of wild Hizikia fusiformis(Sargassaceae, Phaeophyta) along China's coast. Journal of Applied Phycology, 24(5):1 287-1 294, https://doi.org/10.1007/s10811-011-9778-9.
Zhang Z, Liu J G, Liu J D. 2002. Study review of Hizikia fusiformis. Marine Fisheries Research, 23(3):67-74. (in Chinese with English abstract)
Zhao F J, Liu F L, Liu J D, Ang P O Jr, Duan D L. 2008.Genetic structure analysis of natural Sargassum muticum(Fucales, Phaeophyta) populations using RAPD and ISSR markers. Journal of Applied Phycology, 20(2):191-198, https://doi.org/10.1007/s10811-007-9207-2.
Zhao F J, Wang X L, Liu J D, Duan D L. 2007. Population genetic structure of Sargassum thunbergii (Fucales, Phaeophyta) detected by RAPD and ISSR markers.Journal of Applied Phycology, 19(5):409-416, https://doi.org/10.1007/s10811-006-9147-2.
Zhu T, Heo H J, Row K H. 2010.Optimization of crude polysaccharides extraction from Hizikia fusiformis using response surface methodology. Carbohydrate Polymers, 82(1):106-110, https://doi.org/10.1016/j.carbpol.2010.04.029.