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ZHANG Wenjing, JIN Weihua, DUAN Delin, ZHANG Quanbin. Structural analysis and anti-complement activity of polysaccharides extracted from Grateloupia livida (Harv.) Yamada[J]. HaiyangYuHuZhao, 2019, 37(3): 806-814

Structural analysis and anti-complement activity of polysaccharides extracted from Grateloupia livida (Harv.) Yamada

ZHANG Wenjing1,2, JIN Weihua1,3, DUAN Delin1,4,5,6, ZHANG Quanbin1,4,6
1 Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;
2 School of Medicine, Zhejiang University, Hangzhou 310058, China;
3 College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, China;
4 Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China;
5 State Key Lab of Seaweed Bioactive Substances, Qingdao 266000, China;
6 Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China
Polysaccharides were extracted from Grateloupia livida (Harv.) Yamada using hot water (extracted product denoted WGW) and then degraded in dilute sulfuric acid (degraded product denoted WGWD). The degraded mixture was then separated into four fractions through anion exchange chromatography on 2-diethylaminoethanol (DEAE)-Bio-Gel Agarose FF gel. Electrospray ionization collision-induced dissociation tandem mass spectrometry (ESI-CID-MS/MS) was performed to elucidate the structural features of all fractions. In combination with nuclear magnetic resonance spectroscopy (NMR) and infrared spectroscopy (IR) data, the major polysaccharide structures were concluded to be μ-carrageenan and κ-carrageenan. μ-Carrageenan usually has a backbone of alternating 1,3-linked β-D-galactopyranose residues sulfated at C-4 and 1,4-linked α-D-galactopyranose residues sulfated at C-6, while κ-carrageenan consists of alternating 1,3-linked β-D-galactopyranose residues sulfated at C-4 and 1,4-linked α-D-3,6-anhydrogalactopyranose residues. Trace ν-carrageenan, composed of 1,3-linked β-D-galactopyranose residues sulfated at C-4 and 1,4-linked α-D-galactopyranose residues sulfated at C-2 and C-6, was also detected. Furthermore, the polysaccharide had a backbone comprising 1,3-linked β-D-galactopyranose and 1,4-linked α-L-galactopyranose sulfated at C-6, which is the agarose precursor. The hydroxy groups in the galactopyranose were partially substituted by methyl and pyruvic acid acetal (PA) groups. The anticomplementary activities of WGW and its derivatives against classical pathways were measured. The native polysaccharides in WGW had higher activities, while the derivatives had much weaker activities. This indicated that the molecular weight and sulfate content were important factors affecting the anti-complement activity.
Key words:    red alga|Grateloupia livida (Harv.) Yamada|mass spectrometry|structure   
Received: 2018-05-12   Revised: 2018-06-13
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