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SILVA J. Paulo, ALVES Celso, PINTEUS Susete, SILVA Joana, VALADO Ana, PEDROSA Rui, PEREIRA Leonel. Antioxidant and antitumor potential of wild and IMTA-cultivated Osmundea pinnatifida[J]. HaiyangYuHuZhao, 2019, 37(3): 825-835

Antioxidant and antitumor potential of wild and IMTA-cultivated Osmundea pinnatifida

SILVA J. Paulo1, ALVES Celso2, PINTEUS Susete2, SILVA Joana2, VALADO Ana3, PEDROSA Rui2,4, PEREIRA Leonel5
1 UCIBIO-REQUIMTE, Department of Chemistry, Faculty of Sciences and Technology, Universidade NOVA de Lisboa, Caparica 2829-516, Portugal;
2 MARE-Marine and Environmental Sciences Centre, School of Tourism and Maritime Technology, Polytechnic Institute of Leiria, Peniche 2520-641, Portugal;
3 Instituto Politécnico de Coimbra, ESTESC-Coimbra Health School, Biomedical Sciences Laboratory, R. 5 de Outubro, S. Martinho do Bispo, Ap. 7006, Coimbra 3046-854, Portugal;
4 Centre of Pharmacology and Chemical Biopathology, Faculty of Medicine, University of Porto, Porto 4200-319, Portugal;
5 MARE-Marine and Environmental Sciences Centre, Department of Life Sciences, Faculty of Sciences and Technology, University of Coimbra, Coimbra 3000-456, Portugal
Osmundea pinnatifida is a red edible seaweed known as pepper dulse. O. pinnatifida was cultivated in the farm of ALGAplus (Ílhavo, Portugal). This farm is integrated with a seabream and seabass commercial aquaculture and uses the nutrient-enriched water resultant from the fish production as its cultivation medium in the integrated multi-trophic aquaculture (IMTA) manner. Wild and IMTA-cultivated samples of O. pinnatifida were screened for antioxidant and antitumor activities. The antioxidant capacity of solvent extracts from wild and IMTA cultivated samples was assessed in two methods (2,2-diphenyl-1-picrylhydrazyl (DPPH) and oxygen radical absorbance capacity (ORAC)), and their total phenolic contents (TPC) were estimated. Antitumor activity was evaluated in three different tumor cell lines (HepG-2, MCF-7, and SH-SY5Y) through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Among the solvents used for extraction, dichloromethane was the most effective to extract phenolic compounds and presented higher ORAC. A significant correlation was found between TPC and ORAC, which was also sustained by the principal components analysis (PCA). Dichloromethane extracts induced a cytostatic effect on MCF-7 cells and showed weak cytotoxicity to SH-SY5Y cells and weak impact on cell proliferation. Overall, there were no statistically significant differences in the biological activities shown by the wild and IMTA-cultivated samples. Hence, O. pinnatifida can be obtained in an economical and environmentally sustainable way through IMTA, maintaining bioactive properties in a high potential for further nutraceutical purposes.
Key words:    edible seaweed|bioactive compounds|polyphenols|cytotoxic activity|antiproliferative activity   
Received: 2018-05-21   Revised: 2018-07-31
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Articles by SILVA J. Paulo
Articles by ALVES Celso
Articles by PINTEUS Susete
Articles by SILVA Joana
Articles by VALADO Ana
Articles by PEDROSA Rui
Articles by PEREIRA Leonel
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