Cite this paper:
YU Yuan, WANG Le, FU Xiaoting, WANG Lei, FU Xiaodan, YANG Min, HAN Zhenlian, MOU Haijin, JEON You-Jin. Anti-oxidant and anti-inflammatory activities of ultrasonic-assistant extracted polyphenol-rich compounds from Sargassum muticum[J]. HaiyangYuHuZhao, 2019, 37(3): 836-847

Anti-oxidant and anti-inflammatory activities of ultrasonic-assistant extracted polyphenol-rich compounds from Sargassum muticum

YU Yuan1, WANG Le1, FU Xiaoting1, WANG Lei2, FU Xiaodan1, YANG Min1, HAN Zhenlian1, MOU Haijin1, JEON You-Jin2
1 College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China;
2 Department of Marine Life Sciences, Jeju National University, Jeju 63243, Republic of Korea
Polypehnol is an important, potentially bioactive component of Sargassum muticum. In this study, ultrasonic assisted extraction of polyphenol-rich substances was performed using a 38% ethanol solution at a solid:liquid ratio of 1:30 at 68℃ for 32 min, determined by single-factor and response surface methodology (RSM) optimization. The content of polyphenol was 5.66 mg/g in the crude extract. Further extraction showed that the polyphenol mainly distributed in ethyl acetate (SKEE) and water phases (SKEW). The anti-oxidation test by electron spin resonance (ESR) spectrum showed that the SKEE had the strongest scavenging activity on DPPH (1,1-diphenyl-2-picrylhydrazyl) and alkyl radicals. SKEE was shown noncytotoxic but could inhibit the generation of cellular ROS, showing protective effects in H2O2 and AAPHinduced Vero cells and UV-B irradiated HaCaT cells. SKEE also significantly inhibited the release of NO of LPS-induced RAW 264.7 cells. Therefore, the polyphenol-rich extracts in ethanol and ethyl acetate showed excellent anti-oxidant and anti-inflammatory activities, which is beneficial to the development of high-value bio-substances.
Key words:    Sargassum muticum|polyphenol|extraction|ESR|anti-oxidant|anti-inflammatory   
Received: 2018-05-14   Revised: 2018-06-23
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