Cite this paper:
YU Yuan, WANG Le, FU Xiaoting, WANG Lei, FU Xiaodan, YANG Min, HAN Zhenlian, MOU Haijin, JEON You-Jin. Anti-oxidant and anti-inflammatory activities of ultrasonic-assistant extracted polyphenol-rich compounds from Sargassum muticum[J]. HaiyangYuHuZhao, 2019, 37(3): 836-847

Anti-oxidant and anti-inflammatory activities of ultrasonic-assistant extracted polyphenol-rich compounds from Sargassum muticum

YU Yuan1, WANG Le1, FU Xiaoting1, WANG Lei2, FU Xiaodan1, YANG Min1, HAN Zhenlian1, MOU Haijin1, JEON You-Jin2
1 College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China;
2 Department of Marine Life Sciences, Jeju National University, Jeju 63243, Republic of Korea
Abstract:
Polypehnol is an important, potentially bioactive component of Sargassum muticum. In this study, ultrasonic assisted extraction of polyphenol-rich substances was performed using a 38% ethanol solution at a solid:liquid ratio of 1:30 at 68℃ for 32 min, determined by single-factor and response surface methodology (RSM) optimization. The content of polyphenol was 5.66 mg/g in the crude extract. Further extraction showed that the polyphenol mainly distributed in ethyl acetate (SKEE) and water phases (SKEW). The anti-oxidation test by electron spin resonance (ESR) spectrum showed that the SKEE had the strongest scavenging activity on DPPH (1,1-diphenyl-2-picrylhydrazyl) and alkyl radicals. SKEE was shown noncytotoxic but could inhibit the generation of cellular ROS, showing protective effects in H2O2 and AAPHinduced Vero cells and UV-B irradiated HaCaT cells. SKEE also significantly inhibited the release of NO of LPS-induced RAW 264.7 cells. Therefore, the polyphenol-rich extracts in ethanol and ethyl acetate showed excellent anti-oxidant and anti-inflammatory activities, which is beneficial to the development of high-value bio-substances.
Key words:    Sargassum muticum|polyphenol|extraction|ESR|anti-oxidant|anti-inflammatory   
Received: 2018-05-14   Revised: 2018-06-23
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