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CARVALHO G. Loïc, SILVA Raquel, GONÇALVES J. Maria, BATISTA T. Maria, PEREIRA Leonel. Extracts of the seaweed Bifurcaria bifurcata display antifungal activity against human dermatophyte fungi[J]. HaiyangYuHuZhao, 2019, 37(3): 848-854

Extracts of the seaweed Bifurcaria bifurcata display antifungal activity against human dermatophyte fungi

CARVALHO G. Loïc1,2,3, SILVA Raquel3, GONÇALVES J. Maria4, BATISTA T. Maria4, PEREIRA Leonel3
1 Environmental and Biochemical Sciences Group, the James Hutton Institute, Dundee DD2 5DA, Scotland, UK;
2 Institute of Mechanical, Process and Energy Engineering, School of Engineering and Physical Sciences, Heriot Watt University, Edinburgh EH14 4AS, Scotland, UK;
3 MARE (Marine and Environmental Sciences Centre), Department of Life Sciences, Faculty of Sciences and Technology, University of Coimbra, Coimbra 3030-790, Portugal;
4 CIEPQPF, Department of Chemical Engineering, Faculty of Science and Technology, University of Coimbra, Coimbra 3030-790, Portugal
Bifurcaria bifurcata is a seaweed of the Order Fucales (Ochrophyta, Phaeophyceae) that can be found all year round along the Portuguese Atlantic shore. Although it is considered edible in some countries, its biotechnological potential has not yet been assessed in detail and here we report its antimicrobial potential against human dermatophyte fungi. Three samples were harvested from Baleal Island (Peniche, Portugal) harvested in winter (BbPe); from Aguda Beach (Arcozelo, Vila Nova de Gaia, Portugal), harvested in autumn (BbAg) and a sample obtained by culturing the Aguda sample under laboratory conditions (BbLC). A broth macrodilution assay was applied to determine the MIC (minimum inhibitory concentration) and MLC (minimum lethal concentration) of the B. bifurcata extracts. Results show that the BbPe MeOH extracts were the most effective and had higher antifungal activity against all the tested dermatophyte strains than the BbLC and BaAg extracts[Epidermophyton floccosum FF9 (100-200 μg/mL MIC; 200 μg/mL MLC), Microsporum canis FF1 (400 μg/mL MIC; ≥ 400 μg/mL MLC), Trichophyton mentagrophytes FF7 (100 μg/mL MIC; >800 μg/mL MLC), M. gypseum CECT 2908 (800 μg/mL MIC; ≥ 800 μg/mL MLC), T. mentagrophytes var. interdigitale CECT 2958 (800 μg/mL MIC; ≥ 800 μg/mL MLC), T. rubrum CECT 2794 (200 μg/mL MIC; ≥ 400 μg/mL MLC)]. In fact, only E. floccosum FF9 was sensitive to BbAg and BbLC MeOH extracts. To our knowledge, this is the first report of antifungal activity of B. bifurcata against human dermatophyte fungi.
Key words:    seaweed|Bifurcaria bifurcata|bioactivity|antifungal|dermatophytes|minimum inhibitory concentration|minimum lethal concentration   
Received: 2018-05-02   Revised: 2018-08-22
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