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HOU Dandan, YU Weiting, ZHANG Demeng, ZHAO Lili, LIU Xiudong, MA Xiaojun. Culture of yeast cells immobilized by alginate-chitosan microcapsules in aqueous-organic solvent biphasic system[J]. HaiyangYuHuZhao, 2019, 37(3): 863-870

Culture of yeast cells immobilized by alginate-chitosan microcapsules in aqueous-organic solvent biphasic system

HOU Dandan1, YU Weiting2, ZHANG Demeng3, ZHAO Lili3, LIU Xiudong1, MA Xiaojun4
1 College of Environment and Chemical Engineering, Dalian University, Dalian 116622, China;
2 Affiliated Zhongshan Hospital of Dalian University, Dalian 116001, China;
3 State Key Laboratory of Bioactive Seaweed Substances, Qingdao Brightmoon Seaweed Group Co. Ltd., Qingdao 266400, China;
4 Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China
Immobilization biocatalysis is a potential technology to improve the activity and stability of biocatalysts in nonaqueous systems for efficient industrial production. Alginate-chitosan (AC) microcapsules were prepared as immobilization carriers by emulsification-internal gelation and complexation reaction, and their contribution on facilitating the growth and metabolism of yeast cells were testified successfully in culture medium-solvent biphasic systems. The cell growth in AC microcapsules is superior to that in alginate beads, and the cells in both immobilization carriers maintain much higher activity than free cells, which demonstrates AC microcapsules can confer yeast cells the ability to resist the adverse effect of solvent. Moreover, the performance of AC microcapsules in biphasic systems could be improved by adjusting the formation of outer polyelectrolyte complex (PEC) membrane to promote the cell growth and metabolic ability under the balance of resisting solvent toxicity and permitting substrate diffusion. Therefore, these findings are quite valuable for applying AC microcapsules as novel immobilization carriers to realize the biotransformation of value-added products in aqueous-solvent biphasic systems.
Key words:    alginate-chitosan (AC) microcapsules|immobilization biocatalysis|aqueous-solvent biphasic system|cell growth   
Received: 2018-05-09   Revised: 2018-09-20
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