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DIN Siti Sabariah, CHEW Kit Wayne, CHANG Yu-Kaung, SHOW Pau Loke, PHANG Siew Moi, JUAN Joon Ching. Extraction of agar from Eucheuma cottonii and Gelidium amansii seaweeds with sonication pretreatment using autoclaving method[J]. HaiyangYuHuZhao, 2019, 37(3): 871-880

Extraction of agar from Eucheuma cottonii and Gelidium amansii seaweeds with sonication pretreatment using autoclaving method

DIN Siti Sabariah1,5, CHEW Kit Wayne1, CHANG Yu-Kaung2, SHOW Pau Loke1, PHANG Siew Moi3,4, JUAN Joon Ching5
1 Department of Chemical and Environmental Engineering, Faculty of Engineering, University of Nottingham Malaysia, Jalan Broga, Semenyih 43500, Selangor Darul Ehsan, Malaysia;
2 Department of Chemical Engineering, Ming Chi University of Technology, New Taipei, Taiwan 24301, China;
3 Institute of Ocean and Earth Sciences, University of Malaya, Kuala Lumpur 50603, Malaysia;
4 Institute of Biological Sciences, University of Malaya, Kuala Lumpur 50603, Malaysia;
5 Nanotechnology & Catalysis Research Centre, Institute of Postgraduate Studies, University of Malaya, Kuala Lumpur 50603, Malaysia
The effect of sonication pretreatment condition on Eucheuma cottonii and Gelidium amansii seaweed towards agar extraction wae studied. Four parameters were changed during sonication to investigate the effects on agar yield and quality. These parameters include the time interval, concentration ratio, frequency, and intensity. The highest amount of agar extracted from Eucheuma cottonii species could be obtained from the time interval of 30 min, seaweed weight to solvent volume ratio of 1:20, the frequency of 35 Hz, and the sonication power intensity of 30%. For Gelidium amansii species, the best agar yield also could be obtained from the time interval of 30 min, 1:20 of seaweed weight to water volume ratio, the frequency of 35 Hz, and power intensity of 30%. From the experiment, sonication pretreatment significantly influenced the yield and properties of extracted agar. The sonication with autoclaved seaweed produced agar containing less sulfate content, which is an excellent chemical property for gel electrophoresis applications. The gel strength of sonication with autoclaving for both seaweeds, Eucheuma and Gelidium species was the highest among those by sonication with direct heating, which proved that sonication pretreatment with autoclaving could enhance the physical properties of the agar.
Key words:    Gelidium amansii|Eucheuma cottonii|autoclaving|sonication pretreatment|water extraction   
Received: 2018-05-15   Revised: 2018-08-01
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