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COTAS João, FIGUEIRINHA Artur, PEREIRA Leonel, BATISTA Teresa. The effect of salinity on Fucus ceranoides (Ochrophyta, Phaeophyceae) in the Mondego River (Portugal)[J]. HaiyangYuHuZhao, 2019, 37(3): 881-891

The effect of salinity on Fucus ceranoides (Ochrophyta, Phaeophyceae) in the Mondego River (Portugal)

COTAS João2, FIGUEIRINHA Artur3,4, PEREIRA Leonel1,2, BATISTA Teresa5
1 Department of Life Sciences, University of Coimbra, Coimbra 3001-456, Portugal;
2 MARE-Marine and Environmental Sciences Centre, Faculty of Science and Technology, University of Coimbra, Coimbra 3000-548, Portugal;
3 Laboratory of Pharmacognosy, Faculty of Pharmacy, University of Coimbra, Coimbra 3000-548, Portugal;
4 LAQV, REQUIMTE, Faculty of Pharmacy, University of Coimbra, Coimbra 3000-548, Portugal;
5 CIEPQPF, Department of Chemical Engineering, Faculty of Science and Technology, University of Coimbra, Coimbra 3030-790, Portugal
Algae (and their extracts) are increasingly important for pharmaceutical applications due to the diversity of useful compounds they contain. The genus Fucus contains one of the most studied species, Fucus vesiculosus. The species F. ceranoides differs from the others of the genus by presenting longitudinal air-vesicles and a capacity to survive at low salinities. It is an alga that inhabits the Mondego River estuary (Portugal), at the southern limit of its distribution, and can serve as a role model to understand the effect of a salt gradient on the production of bioactive compounds. We assessed the phenolic content and antioxidant activity of different F. ceranoides extracts (e.g. methanolic, aqueous and polysaccharide) prepared from samples harvested from two different zones to evaluate if the adaptation of F. ceranoides to different salinity levels influenced its chemical composition. The antioxidant activity of the extracts was determined using 1,2-diphenyl-picrylhydrazyl (DPPH) and 2.2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radicals. These assays demonstrated that the methanolic extract of lyophilized F. ceranoides that grew at low salinities was the most bioactive, i.e. DPPH (IC50=50.39 μg/mL) and ABTS (TEAC=2.42). The total phenolic content (Folin-Ciocalteu method) and the methanolic extract of the lyophilized F. ceranoides collected from a low salinity habitat exhibited the highest phenolic content (PGE=49.48 μg/mg of lyophilized extract) amongst those sampled. Thin layer chromatography (TLC) and Fourier transform infrared spectroscopy (FTIR) were used for the identification of compounds in the extracts. This characterization allowed confirmation that the various extracts contained almost the same compounds but with notable quantitative differences. Based on these results, we conclude that there were differences in the quantity of the compounds due to the effect of salinity. The drying methods used were also found to have influenced the quality of the extracted compounds.
Key words:    Fucus ceranoides|salinity gradient|sequential extractions|phenolic content|dependent bioactivities|spectroscopic analysis   
Received: 2018-04-27   Revised: 2018-06-20
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