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ZHANG Yue, SONG Xiuxian, YU Zhiming, ZHANG Peipei, CAO Xihua, YUAN Yongquan. Impact assessment of modified clay on embryo-larval stages of turbot Scophthalmus maximus L.[J]. HaiyangYuHuZhao, 2019, 37(3): 1051-1061

Impact assessment of modified clay on embryo-larval stages of turbot Scophthalmus maximus L.

ZHANG Yue1,2,4, SONG Xiuxian1,2,3,4, YU Zhiming1,2,3,4, ZHANG Peipei1,2,3,4, CAO Xihua1,2,4, YUAN Yongquan1,2,4
1 CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;
2 Laboratory of Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China;
3 University of Chinese Academy of Sciences, Beijing 100049, China;
4 Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China
Using modified clay is one of the most promising methods for the mitigation of harmful algal blooms (HABs). However, the environmental impact of modified clay has become a subject of global concern. In this study, turbot (Scophthalmus maximus L.) embryos were used as a model to assess the effect of modified clay on this sensitive stage of fish development. Results show that the 24 and 48h LC50 (median lethal concentrations) of the modified clay were 1.70 and 1.65 g/L, and the safe concentration was 0.47 g/L, which is much higher than the concentration used to treat HAB. The modified clay did not affect significantly the hatchability of turbot embryos but when the concentration exceeded 0.50 g/L, the deformity rate of newly hatched larvae increased significantly. The total length, specific growth rate (SGR) and yolk sac absorption rate of larvae reached their peaks at 0.50 g/L and then gradually decreased as the concentration of modified clay increased. Therefore, a moderate amount of modified clay does not harm the survival and hatching of turbot embryos, or the growth and development of newly hatched larvae.
Key words:    modified clay|turbot|embryo|larvae|survival|growth   
Received: 2018-03-05   Revised: 2018-07-05
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American Society for Testing and Materials. 1992. Standard guide for conducting the frog embryo teratogenesis assayXenopus (FETAX). In:American Society for Testing and Materials ed. Annual Book of ASTM Standards.Philadelphia:American Society for Testing and Materials.p.1 199-1 209.
Anderson D M, Glibert P M, Burkholder J M. 2002. Harmful algal blooms and eutrophication:nutrient sources, composition, and consequences. Estuaries, 25(4):704-726.
Anderson D M. 1997. Turning back the harmful red tide.Nature, 388(6642):513-514.
Archambault M C, Bricelj V M, Grant J, Anderson D M. 2004.Effects of suspended and sedimented clays on juvenile hard clams, Mercenaria mercenaria, within the context of harmful algal bloom mitigation. Marine Biology, 144(3):553-565.
Attramadal K J K, Tøndel B, Salvesen I, Øie G, Vadstein O, Olsen Y. 2012. Ceramic clay reduces the load of organic matter and bacteria in marine fish larval culture tanks.Aquacultural Engineering, 49:23-34.
Barcarolli I F, Martinez C B R. 2004. Effects of aluminum in acidic water on hematological and physiological parameters of the neotropical fish Leporinus macrocephalus (Anostomidae). Bulletin of Environmental Contamination and Toxicology, 72(3):639-646.
Bruno D W, Dear G, Seaton D D. 1989. Mortality associated with phytoplankton blooms among farmed Atlantic salmon, Salmo salar L., in Scotland. Aquaculture, 78(3-4):217-222.
Cao L, Huang W, Shan X X, Xiao Z Z, Wang Q Y, Dou S Z. 2009. Cadmium toxicity to embryonic-larval development and survival in red sea bream Pagrus major. Ecotoxicology and Environmental Safety, 72(7):1 966-1 974.
Chai S L, Robinson J, Chong Mei F C. 2014. A review on application of flocculants in wastewater treatment.Process Safety and Environmental Protection, 92(6):489-508.
Dambal V Y, Selvan K P, Lite C, Barathi S, Santosh W. 2017.Developmental toxicity and induction of vitellogenin in embryo-larval stages of zebrafish (Danio rerio) exposed to methyl Paraben. Ecotoxicology and Environmental Safety, 141:113-118.
Delegrange A, Vincent D, Duret M, Amara R. 2015. The use of mussels for mitigating the noxious effect of phytoplankton spring blooms on farmed fish. Aquacultural Engineering, 66:52-61.
Finney D J. 1971. Probit Analysis. 3rd edn. Cambridge University Press, Cambridge. p.197-199.
Fraysse B, Mons R, Garric J. 2006. Development of a zebrafish 4-day toxicity of embryo-larval bioassay to assess chemicals. Ecotoxicology and Environmental Safety, 63(2):253-267.
Gao Y H, Yu Z M, Song X X, Cao X X. 2007. Impact of modified clays on the infant oyster (Crassostrea gigas).Marine Science Bulletin, 26:53-60. (in Chinese with English abstract)
Gibbs M, Özkundakci D. 2011. Effects of a modified zeolite on P and N processes and fluxes across the lake sedimentwater interface using core incubations. Hydrobiologia, 661(1):21-35.
Greig S M, Sear D A, Smallman D, Carling P A. 2005. Impact of clay particles on the cutaneous exchange of oxygen across the chorion of Atlantic salmon eggs. Journal of Fish Biology, 66(6):1 681-1 691.
Hamm J T, Hinton D E. 2000. The role of development and duration of exposure to the embryotoxicity of diazinon.Aquatic Toxicology, 48(4):403-418.
Harvard University. 2007. A summary of error propagation.Physical Sciences 2.
Hugh A. Poston. 1991. Effects of dietary aluminum on growth and composition of young Atlantic salmon. North American Journal of Aquaculture, 53(1):7-10.
James R, Sampath K. 1999. Effect of zeolite on the reduction of cadmium toxicity in water and a freshwater fish, Oreochromis mossambicus. Bulletin of Environmental Contamination and Toxicology, 62(2):222-229.
Jezierska B, Ługowska K, Witeska M. 2009. The effects of heavy metals on embryonic development of fish (a review). Fish Physiology and Biochemistry, 35(4):625-640.
Jia Y D, Meng Z, Liu X F, Lei J L. 2014. Biochemical composition and quality of turbot (Scophthalmus maximus) eggs throughout the reproductive season. Fish Physiology and Biochemistry, 40(4):1 093-1 104.
Jin D, Thunberg E, Hoagland P. 2008. Economic impact of the 2005 red tide event on commercial shellfish fisheries in New England. Ocean & Coastal Management, 51(5):420-429.
Kent M L, Whyte J N C, Latrace C. 1995. Gill lesions and mortality in seawater pen-reared Atlantic salmon Salmo salar associated with a dense bloom of Skeletonema costatum and Thalassiosira species. Diseases of Aquatic Organisms, 22(1):77-81.
Kim W S, Oh M J, Jung S J, Kim Y J, Kitamura S I. 2005.Characterization of an iridovirus detected from cultured turbot Scophthalmus maximus in Korea. Diseases of Aquatic Organisms, 64(2):175-180.
Landsberg J H. 2002. The effects of harmful algal blooms on aquatic organisms. Reviews in Fisheries Science, 10(2):113-390.
Larkin S L, Adams C M. 2007. Harmful algal blooms and coastal business:economic consequences in Florida.Society & Natural Resource, 20(9):849-859.
Larraza I, Peinado C, Abrusci C, Catalina F, Corrales T. 2011.Hyperbranched polymers as clay surface modifiers for UV-cured nanocomposites with antimicrobial activity.Journal of Photochemistry and Photobiology A:Chemistry, 224(1):46-54.
Lee C K, Kim W S, Park Y T, Jo Q T. 2013a. Effect of yellow clay on the oxygen consumption rate of Korean rockfish, Sebastes schlegelii. Journal of the Korean Society of Marine Environment & Safety, 19(3):241-247.
Lee Y C, Jin E S, Jung S W, Kim Y M, Chang K S, Yang J W, Kim S W, Kim Y O, Shin H J. 2013b. Utilizing the algicidal activity of aminoclay as a practical treatment for toxic red tides. Science Reports, 3:1 292.
Lee Y J, Choi J K, Kim E K, Youn S H, Yang E J. 2008. Field experiments on mitigation of harmful algal blooms using a Sophorolipid-Yellow clay mixture and effects on marine plankton. Harmful Algae, 7(2):154-162.
Lewis M A, Dantin D D, Walker C C, Kurtz J C, Greene R M. 2003. Toxicity of clay flocculation of the toxic dinoflagellate, Karenia brevis, to estuarine invertebrates and fish. Harmful Algae, 2(4):235-246.
Lind U T, Chrzanowski T H, Dávalos-Lind L. 1997. Clay turbidity and the relative production of bacterioplankton and phytoplankton. Hydrobiologia, 353(1-3):1-18.
Liu Y, Cao X H, Yu Z M, Song X X, Qiu L X. 2016. Flocculation of harmful algal cells using modified clay:effects of the properties of the clay suspension. Journal of Applied Phycology, 28(3):1 623-1 633.
Lugowska K. 2007. The effect of cadmium and cadmium/copper mixture during the embryonic development on deformation common carp larvae. Electronic Journal of Ichthyology, 2:46-60.
Malakul P, Srinivasan K R, Wang H Y. 1998. Metal adsorption and desorption characteristics of surfactant-modified clay complexes. Industrial & Engineering Chemistry Research, 37(11):4 296-4 301.
Marty G D, Núñez J, Lauren D J, Hinton D E. 1990. Agedependent changes in toxicity of N-nitroso compounds to Japanese medaka (Oryzias latipes) embryos. Aquatic Toxicology, 17(1):45-62.
Monette M Y, Björnsson B T, McCormick S D. 2008. Effects of short-term acid and aluminum exposure on the parrsmolt transformation in Atlantic salmon (Salmo salar):disruption of seawater tolerance and endocrine status.General and Comparative Endocrinology, 158(1):122-130.
Orizar I S, Rivera P P L, Azanza R V. 2013. Harmful algal bloom (HAB) mitigation using ball clay:effect on nontarget organisms. Journal of Environmental Science and Management, 5(2):36-43.
Pan G, Chen J, Anderson D M. 2011. Modified local sands for the mitigation of harmful algal blooms. Harmful Algae, 10(4):381-387.
Park T G, Lim W A, Park Y T, Lee C K, Jeong H J. 2013.Economic impact, management and mitigation of red tides in Korea. Harmful Algae, 30(S1):S131-S143.
Parkyn S M, Hickey C W, Clearwater S J. 2011. Measuring sub-lethal effects on freshwater crayfish (Paranephrops planifrons) behaviour and physiology:laboratory and in situ exposure to modified zeolite. Hydrobiologia, 661(1):37-53.
Paulsen H, Poulsen N E, Iglesias J, Olmedo M, Korsgaard B, Lavens P, Burkhardt-Holm P. 1998. Indicators of nutritional status of turbot Scophthalmus maximus (L., 1758) larvae. Boletin-Instituto Espanol de Oceanografia, 14(1):5-18.
Priede I G, Tytler P. 1977. Heart rate as a measure of metabolic rate in teleost fishes; Salmo gairdneri, Salmo trutta and Gadus morhua. Journal of Fish Biology, 10(3):231-242.
Rybicka E H, Calmano W, Breeger A. 1995. Heavy metals sorption/desorption on competing clay minerals; an experimental study. Applied Clay Science, 9(5):369-381.
Sengco M R, Anderson D M. 2004. Controlling harmful algal blooms through clay flocculation. The Journal of Eukaryotic Microbiology, 51(2):169-172.
Sfakianakis D G, Renieri E, Kentouri M, Tsatsakis A M. 2015.Effect of heavy metals on fish larvae deformities:a review.Environmental Research, 137:246-255.
Song X X, Yu Z M, Gao Y H. 2003. Removal of different species of red tide organisms with an effective claycomplex system. Chinese Journal of Applied Ecology, 14(7):1 165-1 168. (in Chinese)
Stuart K, Rotman F, Drawbridge M. 2016. Methods of microbial control in marine fish larval rearing:clay-based turbidity and passive larval transfer. Aquaculture Research, 47(8):2 470-2 480.
Sun X X, Zhang B. 2000. Toxicity study of anti red tide agents to Penaeus chinensis. Marine Environmental Science, 19(4):5-8. (in Chinese with English abstract)
Thangaraja M, Al-Aisry A, Al-Kharusi L. 2007. Harmful algal blooms and their impacts in the middle and outer ROPME sea area. International Journal of Oceans & Oceanography, 2(1):85-98.
Urban E R Jr, Kirchman D L. 1992. Effect of kaolinite clay on the feeding activity of the eastern oyster Crassostrea virginica (Gmelin). Journal of Experimental Marine Biology and Ecology, 160(1):47-60.
Wang D S, Sun W, Xu Y, Tang H X, Gregory J. 2004. Speciation stability of inorganic polymer flocculant-PACl. Colloids and Surfaces A:Physicochemical and Engineering Aspects, 243(1-3):1-10.
Wang X Q. 2012. Effects of dimethylhydantoin and carbohydrate on growth and immunity of Litopenaeus vannamei. Advanced Materials Research, 356-360:146-151.
Wang Z B, Zhang H G, Pan G. 2016. Ecotoxicological assessment of flocculant modified soil for lake restoration using an integrated biotic toxicity index. Water Research, 97:133-141.
Wang Z F, Yu Z M, Song X X, Cao X H, Liu K. 2014b. Impact of modified clay on the growth of the infant Apostichopus japonicas selenka in habs controling. Oceanologia et Limnologia Sinica, 45(2):233-238. (in Chinese with English abstract)
Wang Z F, Yu Z M, Song X X, Cao X H. 2014a. Effects of modified clay on the infant of Patinopecten yessoensis for HABs control. Marine Environmental Science, 33(6):817-821. (in Chinese with English abstract)
Yıldırım Ö, Türker A, Şenel B. 2009. Effects of natural zeolite(clinoptilolite) levels in fish diet on water quality, growth performance and nutrient utilization of tilapia (Tilapia zillii) Fry. Fresenius Environmental Bulletin, 18(9):1 567-1 571.
Yu Z M, Song X X, Cao X H, Liu Y. 2017. Mitigation of harmful algal blooms using modified clays:theory, mechanisms, and applications. Harmful Algae, 69:48-64.
Yu Z M, Zou J Z, Ma X N. 1994. A new method to improve the capability of clays for removing red tide organisms.Oceanologia et Limnologia Sinica, 25(2):226-232. (in Chinese with English abstract)