Glutathione S-transferase (<i>GST</i>) gene expression profiles in two marine bivalves exposed to BDE-47 and their potential molecular mechanisms -- Journal of Oceanology and Limnology,2015,33(3):705-713

	Cite this paper:
	LI Fei, WU Huifeng, WANG Qing, LI Xuehua, ZHAO Jianmin. Glutathione S-transferase (GST) gene expression profiles in two marine bivalves exposed to BDE-47 and their potential molecular mechanisms[J]. Journal of Oceanology and Limnology, 2015, 33(3): 705-713

Glutathione S-transferase (GST) gene expression profiles in two marine bivalves exposed to BDE-47 and their potential molecular mechanisms

LI Fei¹, WU Huifeng¹, WANG Qing¹, LI Xuehua², ZHAO Jianmin¹

1 Key Laboratory of Coastal Zone Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences; Shandong Provincial Key Laboratory of Coastal Zone Environmental Processes, Yantai 264003, China;
2 Key Laboratory of Industrial Ecology and Environmental Engineering (MOE), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China

Abstract:

Glutathione S-transferases (GSTs) are phase II enzymes that facilitate the detoxification of xenobiotics and play important roles in antioxidant defense. We investigated the expression patterns of seven Venerupis philippinarum GSTs (VpGSTs) and four Mytilus galloprovincialis GSTs(MgGSTs) following exposure to BDE-47. Differential expressions of the seven VpGSTs and four MgGSTs transcripts were observed, with differences between the hepatopancreas and gills. Among these GSTs, the sigma classes (VpGSTS1, VpGSTS2, VpGSTS3, MgGST1, and MgGST3) were highly expressed in response to BDE-47 exposure, demonstrating their potential as molecular biomarkers for environmental biomonitoring studies. We obtained the three-dimensional crystal structures of VpGSTs and MgGSTs by homologous modeling. A model to elucidate the binding interactions between the ligands and receptors was defined by molecular docking. Hydrophobic and π were the most often observed interactions between BDE-47 and the GSTs.

Received: 2014-05-26 Revised: 2014-09-19

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Articles by WU Huifeng

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Articles by LI Xuehua

Articles by ZHAO Jianmin

References:
Bi X H, Qu W Y, Sheng G Y, Zhang W B, Mai B X, Chen D J,Yu L, Fu J M. 2006. Polybrominated diphenyl ethers in South China maternal and fetal blood and breast milk.Environ. Pollut., 144 : 1 024-1 030.
Board P G, Coggan M, Chelvanayagam G, Easteal S, Jermiin L S, Schulte G K, Danley D E, Hoth L R, Griffor M C,Kamath A V, Rosner M H, Chrunyk B A, Perregaux D E,Gabel C A, Geoghegan K F, Pandit J. 2000. Identification, characterization, and crystal structure of the omega class glutathione transferases. J. Biol. Chem., 275 : 24 798-24 806.
Boutet I, Tanguy A, Moraga D. 2004. Characterisation and expression of four mRNA sequences encoding glutathione S-transferases pi, mu, omega and sigma classes in the Pacific oyster Crassostrea gigas exposed to hydrocarbons and pesticides. Mar. Biol., 146 : 53-64.
Chen L L, Mu C K, Zhao J M, Wang C L. 2011. Molecular cloning and characterization of two isoforms of cyclophilin A gene from Venerupis philippinarum. Fish Shellfish Immu., 31 : 1 218-1 223.
Conrad S, James O, Reinette S. 2014. An analysis of historical Mussel Watch Programme data from the west coast of the Cape Peninsula, Cape Town. Mar. pollut. Bull., 87 : 374-380.
Cunha I, Mangas-Ramirez E, Guilhermino L. 2007. Effects of copper and cadmium on cholinesterase and glutathione S-transferase activities of two marine gastropods (Monodonta lineata and Nucella lapillus). Comp.Biochem. Phys. C, 145 : 648-657.
Danielson U H, Mannervik B. 1985. Kinetic independence of the subunits of cytosolic glutathione transferase from the rat. Biochem. J., 231 : 263-267.
Darnerud P O. 2003. Toxic effects of brominated flame retardants in man and in wildlife. Environ. Int., 29 : 841-853. deGroot M J, Vermeulen N P E, Mullenders D L J, denKelder G M D O. 1996. A homology model for rat Mu class glutathione S-transferase 4-4. Chem. Res. Toxicol., 9 : 28-40.
Enayati A A, Ranson H, Hemingway J. 2005. Insect glutathione transferases and insecticide resistance. Insect Mol. Biol., 14 : 3-8.
Hayes J D, Flanagan J U, Jowsey I R. 2005. Glutathione transferases. Annu. Rev. Pharmacol., 45 : 51-88.
Hites R A. 2004. Polybrominated diphenyl ethers in the environment and in people: a meta-analysis of concentrations. Environ. Sci. Technol., 38 : 945-956.
Hoarau P, Damiens G, Romeo M, Gnassia-Barelli M, Bebianno M J. 2006. Cloning and expression of a GST-π gene in Mytilus galloprovincialis. Attempt to use the GST-pi transcript as a biomarker of pollution. Comp. Biochem.Phys. C, 143 : 196-203.
Jin J, Liu W Z, Wang Y, Tang X Y. 2008. Levels and distribution of polybrominated diphenyl ethers in plant, shellfish and sediment samples from Laizhou Bay in China.Chemosphere, 71 : 1 043-1 050.
Ketterer B, Coles B, Meyer D J. 1983. The role of glutathione in detoxication. Environ. Health Perspect., 49 : 59-69.
Kim J H, Dahms H U, Rhee J S, Lee Y M, Lee J, Han K N, Lee J S. 2010. Expression profiles of seven glutathione S-transferase (GST) genes in cadmium-exposed river pufferfish (Takifugu obscurus). Comp. Biochem. Phys. C, 151 : 99-106.
Lee K W, Raisuddin S, Rhee J S, Hwang D S, Yu I T, Lee Y M,Park H G, Lee J S. 2008. Expression of glutathione S-transferase (GST) genes in the marine copepod Tigriopus japonicus exposed to trace metals. Aquat. Toxicol., 89 : 158-166.
Lee Y M, Seo J S, Jung S O, Kim I C, Lee J S. 2006. Molecular cloning and characterization of theta-class glutathione S-transferase (GST-T) from the hermaphroditic fish Rivulus marmoratus and biochemical comparisons with alpha-class glutathione S-transferase (GST-A). Biochem.Bioph. Res. Co., 346 : 1 053-1 061.
Li F, Xie Q, Li X H, Li N, Chi P, Chen J W, Wang Z J, Hao C. 2010. Hormone activity of hydroxylated polybrominated diphenyl ethers on human thyroid receptor-beta: in vitro and in silico investigations. Environ. Health Perspect., 118 : 602-606.
Liang L N, He B, Jiang G B, Chen D Y, Yao Z W. 2004.Evaluation of mollusks as biomonitors to investigate heavy metal contaminations along the Chinese Bohai Sea.Sci. Total Environ., 324 : 105-113.
Liu X L, Zhang L B, You L P, Cong M, Zhao J M, Wu H F, Li C H, Liu D Y, Yu J B. 2011. Toxicological responses to acute mercury exposure for three species of Manila clam Ruditapes philippinarum by NMR-based metabolomics.Environ. Toxicol. Pharmacol., 31 : 323-332.
Martinez L, Polikarpov I, Skaf MS. 2008. Only subtle protein conformational adaptations are required for ligand binding to thyroid hormone receptors: simulations using a novel multipoint steered molecular dynamics approach. J.Phy. Chem. B, 112 : 10 741-10 751.
Mazdai A, Dodder N G, Abernathy M P, Hites R A, Bigsby R M. 2003. Polybrominated diphenyl ethers in maternal and fetal blood samples. Environ. Health Perspect., 111 : 1 249-1 252.
Nair P M G, Choi J. 2011. Identification, characterization and expression profiles of Chironomus riparius glutathione S-transferase (GST) genes in response to cadmium and silver nanoparticles exposure. Aquat. Toxicol., 101 : 550-560.
Pan X H, Tang J H, Li J, Guo Z G, Zhang G. 2010. Levels and distributions of PBDEs and PCBs in sediments of the Bohai Sea, North China. J. Environ. Monit., 12 : 1 234-1 241.
Ramos J D A, Nge C, Wah L B, Yan C K. 2001. cDNA cloning and expression of Blo t 11, the Blomia tropicalis allergen homologous to paramyosin. Int. Arch. Allergy Immunol., 126 : 286-293.
Rhee J S, Lee Y M, Hwang D S, Lee K W, Kim I C, Shin K H,Raisuddin S, Lee J S. 2007. Molecular cloning and characterization of omega class glutathione S-transferase (GST-O) from the polychaete Neanthes succinea : biochemical comparison with theta class glutathione S-transferase (GST-T). Comp. Biochem. Phys. C, 146 : 471-477.
Schecter A, Johnson-Welch S, Tung K C, Harris T R, Papke O,Rosen R. 2007. Polybrominated diphenyl ether (PBDE) levels in livers of US human fetuses and newborns. J.Toxicol. Env. Heal. A, 70 : 1-6.
Shallaja M S, D'Silva C. 2003. Evaluation of impact of PAH on a tropical fish, Oreochromis mossambicus using multiple biomarkers. Chemosphere, 53 : 835-841.
Singh S P, Coronella J A, Benes H, Cochrane B J, Zimniak P. 2001. Catalytic function of Drosophila melanogaster glutathione S-transferase DmGSTS1-1 (GST-2) in conjugation of lipid peroxidation end products. Eur. J.Biochem., 268 : 2 912-2 923.
Sippl W. 2002. Development of biologically active compounds by combining 3D QSAR and structure-based design methods. J. Comput. Aid. Mol. Des., 16 : 825-830.
Srivastava S K, Hu X, Xia H, Awasthi S, Amin S, Singh S V. 1999. Metabolic fate of glutathione conjugate of benzo[a] pyrene-(7R,8S)-diol (9S,10R)-epoxide in human liver.Arch. Biochem. Biophys., 371 : 340-344.
Stapleton H M, Kelly S M, Pei R, Letcher R J, Gunsch C. 2009. Metabolism of polybrominated diphenyl ethers (PBDEs) by human hepatocytes in vitro. Environ. Health Perspect., 117 : 197-202.
Tang A H, Tu C P D. 1994. Biochemical-characterization of drosophila glutathione S-transferases D1 and D21. J. Biol.Chem., 269 : 27 876-27 884.
Tian S Y, Zhu L Y, Liu M. 2010. Bioaccumulation and distribution of polybrominated diphenyl ethers in marine species from Bohai Bay, China. Environ. Toxicol. Chem., 29 : 2 278-2 285.
Wan Q, Whang I, Lee J S, Lee J. 2009. Novel omega glutathione S-transferases in disk abalone: characterization and protective roles against environmental stress. Comp.Biochem. Phys. C, 150 : 558-568.
Wan Q, Whang I, Lee J. 2008. Molecular cloning and characterization of three sigma glutathione S-transferases from disk abalone (Haliotis discus). Comp. Biochem.Phys. B, 151 : 257-267.
Wan Y, Hu J Y, Zhang K, An L H. 2008. Trophodynamics of polybrominated diphenyl ethers in the marine food web of Bohai Bay, North China. Environ. Sci. Technol., 42 : 1 078-1 083.
Won E J, Kim R O, Rhee J S, Park G S, Lee J, Shin K H, Lee Y M, Lee J S. 2011. Response of glutathione S-transferase (GST) genes to cadmium exposure in the marine pollution indicator worm, Perinereis nuntia. Comp. Biochem. Phys.C, 154 : 82-92.
Wu G S, Robertson D H, Brooks C L, Vieth M. 2003. Detailed analysis of grid-based molecular docking: a case study of CDOCKER-A CHARMm-based MD docking algorithm.J. Comput. Chem., 24 : 1 549-1 562.
Xue W L, Warshawsky D. 2005. Metabolic activation of polycyclic and heterocyclic aromatic hydrocarbons and DNA damage: a review. Toxicol. App. Pharm., 206 : 73-93.
Zhang L B, Liu X L, Chen L L, You L P, Pei D, Cong M, Zhao J M, Li C H, Liu D Y, Yu J B, Wu H F. 2011b.Transcriptional regulation of selenium-dependent glutathione peroxidase from Venerupis philippinarum in response to pathogen and contaminants challenge. Fish Shellfish Imm., 31 : 831-837.
Zhang L B, Qiu L H, Wu H F, Liu X L, You L P, Pei D, Chen L L, Wang Q, Zhao J M. 2012. Expression profiles of seven glutathione S-transferase (GST) genes from Venerupis philippinarum exposed to heavy metals and benzo[a] pyrene. Comp. Biochem. Phys. C, 155 : 517-527.
Zhang L, Liu X L, You L P, Zhou D, Wang Q, Li F, Cong M, Li L Z, Zhao J M, Liu D Y, Yu J B, Wu H F. 2011a. Benzo(a) pyrene-induced metabolic responses in Manila clam Ruditapes philippinarum by proton nuclear magnetic resonance (1 H NMR) based metabolomics. Environ.Toxicol. Pharm., 32 : 218-225.