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ZHENG Li, TANG Wen-Qiao, ZHANG Ya, GUO Hongyi. Comparative proteomic analysis of olfactory rosettes in anadromous Coilia nasus and resident Coilia nasus[J]. HaiyangYuHuZhao, 2019, 37(4): 1324-1332

Comparative proteomic analysis of olfactory rosettes in anadromous Coilia nasus and resident Coilia nasus

ZHENG Li1,2, TANG Wen-Qiao1,2,3, ZHANG Ya1, GUO Hongyi1
1 Shanghai Universities Key Laboratory of Marine Animal Taxonomy and Evolution, Shanghai 201306, China;
2 National Demonstration Center for Experimental Fisheries Science Education, Shanghai 201306, China;
3 Key Laboratory of Freshwater Fishery Germplasm Resources, Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, China
The Japanese grenadier anchovy (Coilia nasus) undergoes upstream migration to spawning annually but can also be observed in freshwater resident populations. It has been hypothesized that anadromous adult C. nasus may utilize olfactory cues to locate spawning grounds. We firstly performed a comparative proteome analysis about olfactory rosettes in two populations to hunt for the proteomic changes. Among 5 408 identified proteins, 1 515 proteins (629 up-regulated and 886 down-regulated) were differentially expressed. Especially, several proteins and pathways associated with olfactory signaling were found to be significantly differential. Compared with resident C. nasus, the expressions of Golf protein and the sodium/calcium exchanger were significantly up-regulated in anadromous C. nasus. The expression of adenylate cyclase and regulator of G-protein signaling (RGS) were decreased. Our findings suggest a decrease in the expression of cGMP-dependent protein kinase (PKG) in anadromous C. nasus compared to resident C. nasus. The expression of Calmodulin (CaM) was increased and CaM-dependent protein kinase Ⅱ (CaMKⅡ) was decreased. In addition, KEGG pathway enrichment analysis of up-regulated proteins indicated statistically significant difference not only in olfactory transduction but also in the cGMP-PKG signal pathway. Furtherly, we sought out some proteins expressed in the same trend occurring in DEGs (differentially expressed genes) and DEPs (differentially expressed proteins) by doing the integrative analysis of proteome and transcriptome in olfactory rosettes of C. nasus.
Key words:    Coilia nasus|olfaction|spawning migration|proteome   
Received: 2018-05-31   Revised: 2018-08-08
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Bandoh H, Kida I, Ueda H. 2011. Olfactory responses to natal stream water in sockeye salmon by BOLD fMRI. PLoS One, 6(1):e16051.
Barbin G P, Parker S J, McCleave J D. 1998. Olfactory clues play a critical role in the estuarine migration of silverphase American eels. Environmental Biology of Fishes, 53(3):283-291.
Breer H, Boekhoff I, Tareilus E. 1990. Rapid kinetics of second messenger formation in olfactory transduction. Nature, 345(6270):65-68.
Dittman A H, Quinn T P, Nevitt G A, Hacker B, Storm D R. 1997. Sensitization of olfactory guanylyl cyclase to a specific imprinted odorant in coho salmon. Neuron, 19(2):381-389.
Døving K B, Westerberg H, Johnsen P B. 1985. Role of olfaction in the behavioral and neuronal responses of Atlantic salmon, Salmo salar, to hydrographic stratification. Canadian Journal of Fisheries and Aquatic Sciences, 42(10):1 658-1 667.
Hirotsu T, Saeki S, Yamamoto M, Iino Y. 2000. The RasMAPK pathway is important for olfaction in Caenorhabditis elegans. Nature, 404(6775):289-293.
Hu G J, Koh J, Yoo M J, Grupp K, Chen S X, Wendel J F. 2013.Proteomic profiling of developing cotton fibers from wild and domesticated Gossypium barbadense. New Phytologist, 200(2):570-582.
Jiang T, Yang J, Liu H B, Shen X Q. 2012. Life history of Coilia nasus from the Yellow Sea inferred from otolith Sr:Ca ratios. Environmental Biology of Fishes, 95(4):503-508.
Johnstone K A, Lubieniecki K P, Koop B F, Davidson W S. 2011. Expression of olfactory receptors in different life stages and life histories of wild Atlantic salmon (Salmo salar). Molecular Ecology, 20(19):4 059-4 069.
Kaupp U B. 2010. Olfactory signalling in vertebrates and insects:differences and commonalities. Nature Reviews Neuroscience, 11(3):188-200.
Mashukova A, Spehr M, Hatt H, Neuhaus E M. 2006. β-arrestin2-mediated internalization of mammalian odorant receptors. The Journal of Neuroscience, 26(39):9 902-9 912.
Mombaerts P. 1999. Molecular biology of odorant receptors in vertebrates. Annual Review of Neuroscience, 22:487-509.
Moon C, Jaberi P, Otto-Bruc A, Baehr W, Palczewski K, Ronnett G V. 1998. Calcium-sensitive particulate guanylyl cyclase as a modulator of cAMP in olfactory receptor neurons. The Journal of Neuroscience, 18(9):3 195-3 205.
Muers M. 2011. Gene expression:transcriptome to proteome and back to genome. Nature Reviews Genetics, 12(8):518.
Nakamura T, Gold G H. 1987. A cyclic nucleotide-gated conductance in olfactory receptor cilia. Nature, 325(6103):442-444.
Nickell W T, Kleene N K, Kleene S J. 2007. Mechanisms of neuronal chloride accumulation in intact mouse olfactory epithelium. The Journal of Physiology, 583:1 005-1 020.
Noé J, Tareilus E, Boekhoff I, Breer H. 1997. Sodium/calcium exchanger in rat olfactory neurons. Neurochemistry International, 30(6):523-531.
Pace U, Hanski E, Salomon Y, Lancet D. 1985. Odorantsensitive adenylate cyclase may mediate olfactory reception. Nature, 316(6025):255-258.
Peppel K, Boekhoff I, McDonald P, Breer H, Caron M G, Lefkowitz R J. 1997. G protein-coupled receptor kinase 3(GRK3) gene disruption leads to loss of odorant receptor desensitization. The Journal of Biological Chemistry, 272(41):25 425-25 428.
Reisert J, Lai J, Yau K W, Bradley J. 2005. Mechanism of the excitatory Cl- response in mouse olfactory receptor neurons. Neuron, 45(4):553-561.
Reisert J, Matthews H R. 1998. Na+-dependent Ca2+ extrusion governs response recovery in frog olfactory receptor cells.The Journal of General Physiology, 112(5):529-535.
Schwanhäusser B, Busse D, Li N, Dittmar G, Schuchhardt J, Wolf J, Chen W, Selbach M. 2011. Global quantification of mammalian gene expression control. Nature, 473(7347):337-342.
Sinnarajah S, Dessauer C W, Srikumar D, Chen J, Yuen J, Yilma S, Dennis J C, Morrison E E, Vodyanoy V, Kehrl J H. 2001. RGS2 regulates signal transduction in olfactory neurons by attenuating activation of adenylyl cyclase Ⅲ.Nature, 409(6823):1 051-1 055.
Vogel C, Marcotte E M. 2012. Insights into the regulation of protein abundance from proteomic and transcriptomic analyses. Nature Reviews Genetics, 13(4):227-232.
Watt W C, Storm D R. 2001. Odorants stimulate the ERK/mitogen-activated protein kinase pathway and activate cAMP-response element-mediated transcription in olfactory sensory neurons. The Journal of Biological Chemistry, 276(3):2 047-2 052.
Yamamoto Y, Hino H, Ueda H. 2010. Olfactory imprinting of amino acids in lacustrine sockeye salmon. PLoS One, 5(1):e8633,
Yano K, Nakamura A. 1992. Observations on the effect of visual and olfactory ablation on the swimming behavior of migrating adult chum salmon, Oncorhynchus keta.Japanese Journal of Ichthyology, 39(1):67-83.
Zhu G L, Tang W Q, Wang L J, Wang C, Wang X M. 2016.Identification of a uniquely expanded V1R (ORA) gene family in the Japanese grenadier anchovy (Coilia nasus).Marine Biology, 163:126.
Zhu G L, Wang L J, Tang W Q, Liu D, Yang J Q. 2014. De novo transcriptomes of olfactory epithelium reveal the genes and pathways for spawning migration in Japanese grenadier anchovy (Coilia nasus). PLoS One, 9(8):e103832.