Growth inhibition and oxidative stress in two species of marine diatoms exposed to 1-phenylethanol -- Journal of Oceanology and Limnology,2019,37(4):1342-1352

	Cite this paper:
	DOU Xiang, MENG Fanping, DUAN Weiyan, LIU Qunqun, LI Hao, DU Shuhao, PENG Xiaoling. Growth inhibition and oxidative stress in two species of marine diatoms exposed to 1-phenylethanol[J]. Journal of Oceanology and Limnology, 2019, 37(4): 1342-1352

Growth inhibition and oxidative stress in two species of marine diatoms exposed to 1-phenylethanol

DOU Xiang¹, MENG Fanping^1,2, DUAN Weiyan¹, LIU Qunqun¹, LI Hao¹, DU Shuhao¹, PENG Xiaoling¹

1 Key Laboratory of Marine Environment and Ecology, Ministry of Education, Qingdao 266000, China;
2 College of Environmental Science and Engineering, Ocean University of China, Qingdao 266000, China

Abstract:

1-phenylethanol (1-PEA) is a flavor extensively used in the production of cosmetics, beverages, and food. The release of 1-PEA into coastal environments has aroused great concern. However, its potential effects on marine organisms are still unknown. In order to provide a better understanding of the ecological risks of 1-PEA in marine environments, this study determined the toxic effects of 1-PEA on two marine diatoms (Phaeodactylum tricornutum and Skeletonema costatum). The diatoms were grown in culture medium containing different concentrations of 1-PEA for 96 h. The contents of chlorophyll a, chlorophyll c, glutathione (GSH), malondialdehyde (MDA), and the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx), were measured at the end of the exposure period. 1-PEA was shown to significantly inhibit the growth of diatoms, with 96-h EC₅₀ values of 257.14 mg/Land 126.46 mg/L in P. tricornutum and S. costatum, respectively. In P. tricornutum, the levels of SOD, CAT, GPx, GSH, and MDA were stimulated only when 1-PEA concentrations were close to or greater than the 96-h EC₅₀ value. However, in S. costatum, the activities of SOD and CAT, and the syntheses of two chlorophylls were inhibited even at an exposure concentration below the 96-h EC₅₀ value. Taken together, these findings indicate a potential ecological risk by discharging 1-PEA into coastal areas and its species-specific toxic effects on marine organisms.

Key words: Phaeodactylum tricornutum|Skeletonema costatum|1-phenylethanol (1-PEA)|growth inhibition|oxidative stress

Received: 2018-08-15 Revised: 2018-09-06

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