Cite this paper:
GUO Li, LIANG Sijie, ZHANG Zhongyi, LIU Hang, WANG Songwen, YANG Guanpin. Domestication of marine microalga Nannochloropsis oceanica to freshwater medium and the physiological responses[J]. HaiyangYuHuZhao, 2019, 37(4): 1353-1362

Domestication of marine microalga Nannochloropsis oceanica to freshwater medium and the physiological responses

GUO Li1,2, LIANG Sijie1,2, ZHANG Zhongyi1,2, LIU Hang1,2, WANG Songwen4, YANG Guanpin1,2,3
1 College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China;
2 Key Laboratory of Marine Genetics and Breeding of Ministry of Education, Ocean University of China, Qingdao 266003, China;
3 Institutes of Evolution and Marine Biodiversity, Ocean University of China, Qingdao 266003, China;
4 College of Agriculture and Resources and Environment, Tianjin Agricultural University, Tianjin 300384, China
Marine microalga Nannochloropsis oceanica LAMB0001 were domesticated (~730 generations,~two days each) to adapt freshwater BG11 medium. A number of freshwater medium adapted colony-derived strains were obtained. The strains were verified phylogenetically to be N. oceanica LAMB0001 based on the 18S ribosomal RNA gene. Freshwater-medium adapted strain (FA1) grew faster in the BG11 medium prepared with freshwater than wild-type N. oceanica grew in f/2 medium prepared with seawater. We assumed that (1) the expression patterns of the genes that expressed differentially between FA1 and the wild-type N. oceanica exposing to the BG11 medium (WT-F) have been reprogrammed; (2) the physiological processes in which these genes involved have been modified; and (3) a Gene Ontology (GO) term or a KEGG pathway enriched by DEGs between FA1 and WT-F has been up- or down-regulated if it was enriched simultaneously by up- or down-regulated DEGs between FA1 and WT-F, respectively. Under these assumptions, we found that FA1 reprogrammed the expression patterns of a set of genes that involved in cell adhesion, membrane and membrane integrity, material transportation, cell movement, and cellular signaling network. These changes in cellular functions and metabolic pathways indicate that the microalga modified its gene expression pattern in a wide function range and at a high regulation rank in order to adapt to the freshwater medium. It is feasible to domesticate marine microalgae to a freshwater habitat, which may aid to modify their cultivation performances.
Key words:    Nannochloropsis oceanica|domestication|acclimation|adaptation|genetic variation   
Received: 2018-08-06   Revised: 2018-10-05
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