Cite this paper:
Thilina U. JAYAWARDENA, Won Woo LEE, I. P. Shanura FERNANDO, K. K. Asanka SANJEEWA, Lei WANG, Tee Gee LEE, Young Jin PARK, Chang-ik KO, You-Jin JEON. Antiproliferative and apoptosis-inducing potential of 3β-hydroxy-Δ5-steroidal congeners purified from the soft coral Dendronephthya putteri[J]. HaiyangYuHuZhao, 2019, 37(4): 1382-1392

Antiproliferative and apoptosis-inducing potential of 3β-hydroxy-Δ5-steroidal congeners purified from the soft coral Dendronephthya putteri

Thilina U. JAYAWARDENA1, Won Woo LEE1, I. P. Shanura FERNANDO1, K. K. Asanka SANJEEWA1, Lei WANG1, Tee Gee LEE2, Young Jin PARK3, Chang-ik KO4, You-Jin JEON1
1 Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Republic of Korea;
2 Department of Hotel Cooking Nutrition, Jeonnam Provincial College, Jeollanam-do, Republic of Korea;
3 Department of Family Medicine, College of Medicine, Dong-A University, Busan 602-715, Republic of Korea;
4 Choung Ryong Fisheries Co. Ltd., 7825, Iljudong-ro, Namwon-eup, Seogwipo-si, Jeju-do, Republic of Korea
Abstract:
The exploration and identification of antiproliferative phytochemicals have received increased attention in medicinal chemistry. In particular, research focused on the toxicology of marine natural products has increased in recent years. Terpenoids, among many secondary metabolites, have been demonstrated to act as effective anticancer agents. Soft corals, a group of marine invertebrates, produce a variety of terpenoids with biofunctional properties. The current study presents the extraction, purification, and identification of sterol congeners from the soft coral Dendronephthya putteri. The method involves 50% chloroform-methanol extraction, polar column fractionation, and analysis through GC-MSn. Dose-dependent antiproliferative activity was observed within the sterol-rich fraction (DPCMH 2-4), which consisted of 3β-hydroxy-Δ5-steroidal congeners. This fraction inhibited the growth of HL-60 and MCF-7 cells with IC50 values of 25.27±1.43 and 22.81±0.15 μg/mL, respectively. Apoptotic body formation, DNA damage, cell cycle arrest, and apoptotic cell signaling pathway activation were also observed, reinforcing the dose-dependent antiproliferative and apoptosis-inducing activity of 3β-hydroxy-Δ5-steroidal congeners. To our knowledge, this is the first report of anticancer agent identification from the soft coral D. putteri. Based on the observations, these steroidal congeners are promising candidates for the development of anticancer drugs.
Key words:    Dendronephthya putteri|soft coral|antiproliferative agent|HL-60|MCF-7|apoptosis|steroidal congeners   
Received: 2018-04-20   Revised: 2018-09-13
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Articles by Thilina U. JAYAWARDENA
Articles by Won Woo LEE
Articles by I. P. Shanura FERNANDO
Articles by K. K. Asanka SANJEEWA
Articles by Lei WANG
Articles by Tee Gee LEE
Articles by Young Jin PARK
Articles by Chang-ik KO
Articles by You-Jin JEON
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