Cite this paper:
LI Miaoyu, XI Bingwen, QIN Ting, CHEN Kai, XIE Jun. Isolation and characterization of AHL-degrading bacteria from fish and pond sediment[J]. HaiyangYuHuZhao, 2019, 37(4): 1460-1467

Isolation and characterization of AHL-degrading bacteria from fish and pond sediment

LI Miaoyu1,2, XI Bingwen2, QIN Ting2, CHEN Kai2, XIE Jun1,2
1 Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China;
2 Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China
Quorum sensing (QS) disruption is considered as a potential alternative strategy to combat bacterial diseases in aquaculture. In this study, we isolated and identified bacteria degrading QS molecules from pond sediment and fish intestine. A total of 132 strains were obtained in the enrichment culture, of which two strains were identified as Enterobacter sp. f003 and Staphylococcus sp. sw120, being isolated from the fish intestine and pond sediment, respectively. We found that strains f003 and sw120 could degrade acyl-homoserine lactones (AHLs) and cause no hemolysis of sheep red blood cells. The AHL lactonase (aiiA) homologous gene in the two strains was detected in PCR amplification and the high-degrading activity to N-hexanoyl-L-homoserine lactone (C6-HSL) and AHLs secreted from pathogenic Aeromonas hydrophila was assessed. Meanwhile, the artificial infection of cyprinid Carassius auratus gibelio with intraperitoneal injection showed that the two strains were avirulent. Therefore, the obtained indigenous bacteria are candidate probiotics against pathogenic A. hydrophila in aquaculture.
Key words:    quorum sensing (QS)|Aeromonas hydrophila|probiotic|Enterobacter|Staphylococcus   
Received: 2018-05-11   Revised: 2018-09-03
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