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WANG Shuping, ZHANG Yuan, HE Jia, JIA Xiaobo, LIN Jianing, LI Meng, WANG Qinglin. Molecular analyses of bacterioplankton communities with highly abundant Vibrio clades: a case study in Bohai Sea coastal waters[J]. HaiyangYuHuZhao, 2019, 37(5): 1638-1648

Molecular analyses of bacterioplankton communities with highly abundant Vibrio clades: a case study in Bohai Sea coastal waters

WANG Shuping1, ZHANG Yuan1, HE Jia1, JIA Xiaobo1, LIN Jianing1, LI Meng2, WANG Qinglin3
1 State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China;
2 Institute for Advanced Study, Shenzhen University, Shenzhen 518000, China;
3 College of Animal Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao 066000, China
We investigated the bacterioplankton abundance, community composition and the associated Vibrio clades of natural seawater in Bohai Sea coastal waters. Seawater samples (10 L in triplicate) were collected at 0.5, 3, and 5 m depths near the coastal aquaculture zone of the Bohai Sea on May 12, 2016. Real-time PCR and 16S rRNA gene amplicon high-throughput sequencing methods were employed by which 485 operational taxonomic units (OTUs) at a 97% sequence similarity level were generated. Gammaproteobacteria, Alphaproteobacteria, and Bacteroidetes were the most abundant groups, accounting for 49.5%, 23.5%, and 18.9% of the total assemblage, respectively. Obvious variations in Pseudoalteromonas, Vibrio, and Octadecabacter, which were the most abundant genera, could be observed among different samples. Notably, the results of Vibrio-specific real-time PCR indicated that Vibrio had extremely high 16S rRNA gene copy numbers. The 16S rRNA gene sequencing results across all the samples also indicated that they occupied a large proportion of the total assemblage. Both the alpha diversity and major bacterioplankton group Pseudoalteromonas had significant correlations with the concentration of PO43-. Overall, studies on bacterioplankton communities with highly abundant Vibrio clades can provide interesting insight into the microbial function and health assessment of the Bohai Sea coastal ecosystem.
Key words:    Bohai Sea|bacterioplankton|Vibrio|16S rRNA|Illumina MiSeq   
Received: 2018-08-19   Revised: 2018-12-13
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