Cite this paper:
WANG Shuping, ZHANG Yuan, HE Jia, JIA Xiaobo, LIN Jianing, LI Meng, WANG Qinglin. Molecular analyses of bacterioplankton communities with highly abundant Vibrio clades: a case study in Bohai Sea coastal waters[J]. HaiyangYuHuZhao, 2019, 37(5): 1638-1648

Molecular analyses of bacterioplankton communities with highly abundant Vibrio clades: a case study in Bohai Sea coastal waters

WANG Shuping1, ZHANG Yuan1, HE Jia1, JIA Xiaobo1, LIN Jianing1, LI Meng2, WANG Qinglin3
1 State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China;
2 Institute for Advanced Study, Shenzhen University, Shenzhen 518000, China;
3 College of Animal Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao 066000, China
Abstract:
We investigated the bacterioplankton abundance, community composition and the associated Vibrio clades of natural seawater in Bohai Sea coastal waters. Seawater samples (10 L in triplicate) were collected at 0.5, 3, and 5 m depths near the coastal aquaculture zone of the Bohai Sea on May 12, 2016. Real-time PCR and 16S rRNA gene amplicon high-throughput sequencing methods were employed by which 485 operational taxonomic units (OTUs) at a 97% sequence similarity level were generated. Gammaproteobacteria, Alphaproteobacteria, and Bacteroidetes were the most abundant groups, accounting for 49.5%, 23.5%, and 18.9% of the total assemblage, respectively. Obvious variations in Pseudoalteromonas, Vibrio, and Octadecabacter, which were the most abundant genera, could be observed among different samples. Notably, the results of Vibrio-specific real-time PCR indicated that Vibrio had extremely high 16S rRNA gene copy numbers. The 16S rRNA gene sequencing results across all the samples also indicated that they occupied a large proportion of the total assemblage. Both the alpha diversity and major bacterioplankton group Pseudoalteromonas had significant correlations with the concentration of PO43-. Overall, studies on bacterioplankton communities with highly abundant Vibrio clades can provide interesting insight into the microbial function and health assessment of the Bohai Sea coastal ecosystem.
Key words:    Bohai Sea|bacterioplankton|Vibrio|16S rRNA|Illumina MiSeq   
Received: 2018-08-19   Revised: 2018-12-13
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