Cite this paper:
LIU Qi, LI Wenjun, LU Lina, LIU Bin, DU Zhenning, QIN Song. Phycocyanin attenuates X-ray-induced pulmonary inflammation via the TLR2-MyD88-NF-κB signaling pathway[J]. HaiyangYuHuZhao, 2019, 37(5): 1678-1685

Phycocyanin attenuates X-ray-induced pulmonary inflammation via the TLR2-MyD88-NF-κB signaling pathway

LIU Qi1,2, LI Wenjun1, LU Lina3, LIU Bin3, DU Zhenning2, QIN Song1
1 Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China;
2 School of Pharmacy, Yantai University, Yantai 264005, China;
3 School of Nuclear Science and Technology, Lanzhou University, Lanzhou 730000, China
Abstract:
Phycocyanin (PC), a natural algal protein, is reported for having anti-oxidant and antiinflammatory properties. We investigated its ability to attenuate lung inflammation in mice subjected to X-ray radiation. Male C57BL/6 mice were assigned to the control, total body irradiation, PC pretreatment, and PC treatment groups. Mice in the PC pretreatment group were gavaged with 200 mg/kg PC for 7 consecutive days before irradiation, and those in the PC treatment group were gavaged with 200 mg/kg PC for 7 consecutive days after irradiation. Lungs were collected on Day 7 after irradiation exposure. Hematoxylin and eosin staining of mouse lung sections showed considerable inflammation damage 7 days after irradiation compared with the control lung but a reduction in pathological injury in the PC treatment group. Pretreatment or treatment with PC significantly decreased levels of interleukin-6 and tumor necrosis factor-α in the lung, and also increased the relative mRNA expression of superoxide dismutase and glutathione. In vivo, PC significantly reduced the expression of Toll-like receptor TLR2, myeloid differentiation primary response Myd88, and nuclear factor NF-κB, at both the transcriptional and translation level. Taken together, these data indicated that PC attenuated lung inflammatory damage induced by radiation by blocking the TLR2-MyD88-NF-κB signaling pathway. Therefore, PC could be a protective agent against radiation-induced inflammatory damage in normal tissues.
Key words:    phycocyanin|inflammatory|TLR2-MyD88-NF-κB signaling pathway   
Received: 2018-08-15   Revised: 2018-11-10
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Articles by LIU Qi
Articles by LI Wenjun
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