Cite this paper:
LIU Qi, LI Wenjun, LU Lina, LIU Bin, DU Zhenning, QIN Song. Phycocyanin attenuates X-ray-induced pulmonary inflammation via the TLR2-MyD88-NF-κB signaling pathway[J]. HaiyangYuHuZhao, 2019, 37(5): 1678-1685

Phycocyanin attenuates X-ray-induced pulmonary inflammation via the TLR2-MyD88-NF-κB signaling pathway

LIU Qi1,2, LI Wenjun1, LU Lina3, LIU Bin3, DU Zhenning2, QIN Song1
1 Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China;
2 School of Pharmacy, Yantai University, Yantai 264005, China;
3 School of Nuclear Science and Technology, Lanzhou University, Lanzhou 730000, China
Phycocyanin (PC), a natural algal protein, is reported for having anti-oxidant and antiinflammatory properties. We investigated its ability to attenuate lung inflammation in mice subjected to X-ray radiation. Male C57BL/6 mice were assigned to the control, total body irradiation, PC pretreatment, and PC treatment groups. Mice in the PC pretreatment group were gavaged with 200 mg/kg PC for 7 consecutive days before irradiation, and those in the PC treatment group were gavaged with 200 mg/kg PC for 7 consecutive days after irradiation. Lungs were collected on Day 7 after irradiation exposure. Hematoxylin and eosin staining of mouse lung sections showed considerable inflammation damage 7 days after irradiation compared with the control lung but a reduction in pathological injury in the PC treatment group. Pretreatment or treatment with PC significantly decreased levels of interleukin-6 and tumor necrosis factor-α in the lung, and also increased the relative mRNA expression of superoxide dismutase and glutathione. In vivo, PC significantly reduced the expression of Toll-like receptor TLR2, myeloid differentiation primary response Myd88, and nuclear factor NF-κB, at both the transcriptional and translation level. Taken together, these data indicated that PC attenuated lung inflammatory damage induced by radiation by blocking the TLR2-MyD88-NF-κB signaling pathway. Therefore, PC could be a protective agent against radiation-induced inflammatory damage in normal tissues.
Key words:    phycocyanin|inflammatory|TLR2-MyD88-NF-κB signaling pathway   
Received: 2018-08-15   Revised: 2018-11-10
PDF (1211 KB) Free
Print this page
Add to favorites
Email this article to others
Articles by LIU Qi
Articles by LI Wenjun
Articles by LU Lina
Articles by LIU Bin
Articles by DU Zhenning
Articles by QIN Song
Arrieta O, Gallardo-Rincón D, Villarreal-Garza C, Michel R M, Astorga-Ramos A M, Martínez-Barrera L, De La Garza J. 2009. High frequency of radiation pneumonitis in patients with locally advanced non-small cell lung cancer treated with concurrent radiotherapy and gemcitabine after induction with gemcitabine and carboplatin. J.Thorac. Oncol., 4(7):845-852,
Bhat V B, Madyastha K M. 2000. C-phycocyanin:a potent peroxyl radical scavenger in vivo and in vitro. Biochem.Biophys. Res. Commun., 275(1):20-25,
Carmody R J, Chen Y H. 2007. Nuclear factor-κB:activation and regulation during toll-like receptor signaling. Cell.Mol. Immunol., 4(1):31-41.
Chang C J, Yang Y H, Liang Y C, Chiu C J, Chu K H, Chou H N, Chiang B L. 2011. A novel phycobiliprotein alleviates allergic airway inflammation by modulating immune responses. Am. J. Respir. Crit. Care. Med., 183(1):15-25,
Demirel C, Kilciksiz S C, Gurgul S, Erdal N, Yigit S, Tamer L, Ayaz L. 2016. Inhibition of radiation-induced oxidative damage in the lung tissue:may acetylsalicylic acid have a positive role? Inflammation, 39(1):158-165,
Ding N H, Li J J, Sun L Q. 2013. Molecular mechanisms and treatment of radiation-induced lung fibrosis. Curr. Drug Targets, 14(11):1 347-1 356,
Doz E, Noulin N, Boichot E, Guenon I, Fick L, Le Bert M, Lagente V, Ryffel B, Schnyder B, Quesniaux V F J, Couillin I. 2008. Cigarette smoke-induced pulmonary inflammation is TLR4/MyD88 and IL-1R1/MyD88 signaling dependent. J. Immunol., 180(2):1 169-1 178,
Hosseinimehr S J. 2007. Trends in the development of radioprotective agents. Drug Discov. Today, 12(19-20):794-805,
Kim H S, Go H, Akira S, Chung D H. 2011. TLR2-mediated production of IL-27 and chemokines by respiratory epithelial cells promotes bleomycin-induced pulmonary fibrosis in mice. J. Immunol., 187(8):4 007-4 017,
Li C C, Yu Y, Li W J, Liu B, Jiao X D, Song X Y, Lv C J, Qin S. 2017. Phycocyanin attenuates pulmonary fibrosis via the TLR2-MyD88-NF-κB signaling pathway. Sci. Rep., 7(1):5 843,
Li J, Mu S, Mu L, Zhang X, Pang R, Gao S. 2015. Transforming growth factor-beta-1 is a serum biomarker of radiationinduced pneumonitis in esophageal cancer patients treated with thoracic radiotherapy:preliminary results of a prospective study. Onco. Targets Ther., 8:1 129-1 136.
Li Y J, Han Z, Ge L, Zhou C J, Zhao Y F, Wang D H, Ren J, Niu X X, Liang C G. 2016. C-phycocyanin protects against low fertility by inhibiting reactive oxygen species in aging mice. Oncotarget, 7(14):17 393-17 409,
Liu H Z, Yang H Z, Mi S, Cui B, Hua F, Hu Z W. 2010. Toll like receptor 2 mediates bleomycin-induced acute lung injury, inflammation and fibrosis in mice. Acta Pharm.Sinica, 45(8):976-986.
Medzhitov R. 2001. Toll-like receptors and innate immunity.Nature Reviews Immunology, 1:135-145.
Mehta V. 2005. Radiation pneumonitis and pulmonary fibrosis in non-small-cell lung cancer:pulmonary function, prediction, and prevention. Int. J. Radiat. Oncol. Biol. Phys., 63(1):5-24,
Ogawa Y S, Tasaka W, Yamada F, Saito N, Hasegawa T, Miyasho, Ishizaka A. 2007. Role of Toll-like receptor 4 in hyperoxia-induced lung inflammation in mice.Inflammation Research, 56:334-338.
Rong S, Zhao Y T, Bao W, Xiao X, Wang D, Nussler A K, Yan H, Yao P, Liu L G. 2012. Curcumin prevents chronic alcohol-induced liver disease involving decreasing ROS generation and enhancing antioxidative capacity.Phytomedicine, 19(6):545-550,
Roses R E, Xu M, Koski G K, Czerniecki B J. 2008. Radiation therapy and Toll-like receptor signaling:implications for the treatment of cancer. Oncogene, 27:200-207.
Shen W B, Zhu S C, Gao H M, Li Y M, Liu Z K, Li J, Su J W, Wan J. 2013. Low dose volume histogram analysis of the lungs in prediction of acute radiation pneumonitis in patients with esophageal cancer treated with threedimensional conformal radiotherapy. Chin. J. Oncol., 35(1):45-49.
Verma S, Kalita B, Bajaj S, Prakash H, Singh A K, Gupta M L. 2017. A combination of podophyllotoxin and rutin alleviates radiation-induced pneumonitis and fibrosis through modulation of lung inflammation in mice. Front.Immunol., 8:658,
Williams M A, Withington S, Newland A C, Kelsey S M. 1998.Monocyte anergy in septic shock is associated with a predilection to apoptosis and is reversed by granulocytemacrophage colony-stimulating factor ex vivo. J. Infect.Dis., 178(5):1 421-1 433,
Wu Q H, Liu L, Miron A, Klímová B, Wan D, Kuča K. 2016.The antioxidant, immunomodulatory, and antiinflammatory activities of Spirulina:an overview. Arch.Toxicol., 90(8):1 817-1 840,
Zhang X C, Shan P Y, Qureshi S, Homer R, Medzhitov R, Noble P W, Lee P J. 2005. Cutting edge:TLR4 deficiency confers susceptibility to lethal oxidant lung injury. J.Immunol., 175(8):4 834-4 838,