Cite this paper:
LI Yuanxiang, CAI Xuehua, GU Wenhui, WANG Guangce. Transcriptome analysis of carotenoid biosynthesis in Dunaliella salina under red and blue light[J]. HaiyangYuHuZhao, 2020, 38(1): 177-185

Transcriptome analysis of carotenoid biosynthesis in Dunaliella salina under red and blue light

LI Yuanxiang1,2,3, CAI Xuehua1,2,3, GU Wenhui1,2, WANG Guangce1,2
1 Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China;
2 Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China;
3 College of Earth Sciences, University of Chinese Academy of Sciences, Beijing 100049, China
The quality of light is an important abiotic factor that affects the growth and development of photosynthetic organisms. In this study, we exposed the unicellular green alga Dunaliella salina to red (660 nm) and blue (450 nm) light and analyzed the cell growth, total carotenoid content, and transcriptomes. The growth of D. salina was enhanced by illumination with red light, whereas blue light was not able to promote the algal growth. In contrast, the total carotenoid content increased under both red and blue light. The RNA of D. salina was sequenced and the transcriptomic response of algal cells to red and blue light was investigated. Six transcripts encoding for the blue light receptor cryptochrome were identified, and transcripts involved in the carotenoid metabolism were up-regulated under both red and blue light. Transcripts encoding for photoprotective enzymes related to the scavenging of reactive oxygen species were up-regulated under blue light. The present transcriptomic study provides a more comprehensive understanding of carotenoid biosynthesis in D. salina under different wavelengths of light.
Key words:    blue light|carotenoid|Dunaliella|red light|transcriptome   
Received: 2019-03-11   Revised:
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