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REN Shiying, LI Xiangqian, YIN Xiulian, LUO Chuping, LIU Fei. Characteristics of intracellular polyphosphate granules and phosphorus-absorption of a marine polyphosphateaccumulating bacterium, Halomonas sp. YSR-3[J]. Journal of Oceanology and Limnology, 2020, 38(1): 195-203

Characteristics of intracellular polyphosphate granules and phosphorus-absorption of a marine polyphosphateaccumulating bacterium, Halomonas sp. YSR-3

REN Shiying1,3, LI Xiangqian1,3, YIN Xiulian1,3, LUO Chuping1,3, LIU Fei2
1 Jiangsu Provincial Key Construction Laboratory of Probiotics Preparation, Huaiyin Institute of Technology, Huai'an 223003, China;
2 Jiangsu Provincial Engineering Laboratory for Advanced Materials of Salt Chemical Industry, Huaiyin Institute of Technology, Huai'an 223003, China;
3 School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai'an 223003, China
Halomonas sp. YSR-3 was isolated from the Yellow Sea and identified as a polyphosphateaccumulating bacterium and the characteristics of its intracellular polyphosphate (polyP) granules and phosphorus absorption were studied. Most YSR-3 cells stored one or two polyP granules in regular appearance and high-density. The diameter of the granules was about 400 nm measuring by a transmission electron microscope (TEM). After stained with 4,6-diamidino-2-phenylindole (DAPI) and visualized by a fluorescence microscope, the cells turned blue and the granules were bright yellow. The composition of granules includes P (major ingredient), Mg, S, K, and Ca as detected by an energy dispersive X-ray spectrometer (EDS). When inorganic phosphorus (PO43-) and ferric ion (Fe3+) were added into media, the biomass increased and the cells formed intracellular polyP granules owing to the phosphorus assimilation from media. The YSR-3 obtained higher biomass by adding 0.02 g/L FePO4 than 0.005 g/L and 0.01 g/L FePO4; however, the phosphorus absorption was higher with 0.01 g/L FePO4 than 0.005 g/L and 0.02 g/L FePO4. The optical density at wavelength 480 nm (OD480 nm) was 0.79 and 100% cells could form intracellular polyP granules. These results show that strain YSR-3 is able to acquire higher biomass and absorb more inorganic phosphorus when 0.01 g/L FePO4 is added. The characteristics of absorbing and storing phosphorus as intracellular inorganic polyP granules have a potential for application in high-efficiency phosphorus removal in wastewater treatment.
Key words:    polyphosphate-accumulating bacterium|polyphosphate granule|Halomonas|enhanced biological phosphorus removal   
Received: 2018-11-06   Revised:
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