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JIANG Xiaoli, JING Xiaohong, L� Yan, SHAN Changmin, LI Jiahua, YU Yan, WU Yuyong, SUN Yeying. Genome-wide identification and prediction of long noncoding RNAs in half-smooth tongue sole Cynoglossus semilaevis[J]. HaiyangYuHuZhao, 2020, 38(1): 226-235

Genome-wide identification and prediction of long noncoding RNAs in half-smooth tongue sole Cynoglossus semilaevis

JIANG Xiaoli1, JING Xiaohong1, L� Yan2, SHAN Changmin1, LI Jiahua1, YU Yan1, WU Yuyong1, SUN Yeying1
1 College of Pharmacy, Binzhou Medical University, Yantai 264003, China;
2 College of Life Science, Yantai University, Yantai 264005, China
At present, the function and profile of long noncoding RNAs (lncRNAs) in half-smooth tongue soles Cynoglossus semilaevis remain poorly understood. Therefore, we identified lncRNAs in the species using large-scale deep sequencing approaches. A total of 96 726 222 clean reads and 2497 lncRNAs were obtained from the compound samples. In total, 1 694 known lncRNAs and 803 novel lncRNAs were identified. Most of the novel lncRNAs distributed mainly in a range of 200-3 000 nt and contained 2-6 exons. Six novel lncRNAs were selected for expression analysis by quantitative reverse transcription-polymerase chain reaction, of which lnc_770 and lnc_150 were expressed mainly in the ovary. This study provides a valuable resource for lncRNA studies of half-smooth tongue sole and improves our understanding of the function of non-coding RNA in fish.
Key words:    Cynoglossus semilaevis|long noncoding RNAs (lncRNAs)|expression profiles   
Received: 2018-10-17   Revised:
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